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The map label for this gene is sleB [H]

Identifier: 167040813

GI number: 167040813

Start: 2188382

End: 2189077

Strand: Reverse

Name: sleB [H]

Synonym: Teth514_2190

Alternate gene names: 167040813

Gene position: 2189077-2188382 (Counterclockwise)

Preceding gene: 167040814

Following gene: 167040812

Centisome position: 89.09

GC content: 35.63

Gene sequence:

>696_bases
ATGACAATATTGATAGAAGAGTTTATAATTGTAATCGAATTAACTCCTGCTATTGCGGTTAACGGAATAGAAAGGAGGAA
ACTTATGATGAAGAATAAAATTTTGAAAATTGCTGTAGTATATTTAATAATTTTTATAATGATATTTGGAGGAATTACTT
CGGCATACGCTTACGACAAAATCCACGTTGTTTCTAAAGGTGAATCTTTGTATTTAATAGCGAAATGGTATGGTAGTGAT
GTAAATAGCATAAAACAGGCAAATGGGAAATGGAGCGATTTGATATATCCTGGTGAAAAATTGGTTGTGCCAGTAAATGA
AAATAGTGATTATTACAATTATCTTGTTGACAGATATTTAATCGCAAAAATGATTTATGCTGAAGCTCGTGGAGAAAGCT
TTGAGGGTCAGGTCGCTGTAGGGGCGGTAATTTTGAATAGAGTAAAAAGCGGTATTTTCCCAAATACTGTAGCAGGAGTT
ATATATCAGCCAGATGCTTTTGAACCTGTAACGAATGGTGAATTTTTCAATCATGAACCTGACCTTACAGCTTTTAAAGC
AGCAGATGCAGCTTTAGCTGGATGGGACCCAACAGGAGGAGCACTATACTTTTTCAATCCAGCTACTTCAACTTCTTGGT
GGATATGGACAAGGACAATTACAAATGTGATAGGACACCACTGGTTCGGAATATAG

Upstream 100 bases:

>100_bases
GGGAATCTTCTATAGATTAGTATATCTAAGAATATATCACTATAATATATCATTCTAGAGATATTTTTAATAAATGGAGG
ATTATCGGGAAATACGAAGG

Downstream 100 bases:

>100_bases
GAAGGCATCATGCCTTCCTATATTTCATATAATTGGCATTATAAAATTTAAGTTTAGGAAAAGAATAAAAATAGTACTCA
AAAATGGGAGCTGGTTATAT

Product: cell wall hydrolase SleB

Products: NA

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTILIEEFIIVIELTPAIAVNGIERRKLMMKNKILKIAVVYLIIFIMIFGGITSAYAYDKIHVVSKGESLYLIAKWYGSD
VNSIKQANGKWSDLIYPGEKLVVPVNENSDYYNYLVDRYLIAKMIYAEARGESFEGQVAVGAVILNRVKSGIFPNTVAGV
IYQPDAFEPVTNGEFFNHEPDLTAFKAADAALAGWDPTGGALYFFNPATSTSWWIWTRTITNVIGHHWFGI

Sequences:

>Translated_231_residues
MTILIEEFIIVIELTPAIAVNGIERRKLMMKNKILKIAVVYLIIFIMIFGGITSAYAYDKIHVVSKGESLYLIAKWYGSD
VNSIKQANGKWSDLIYPGEKLVVPVNENSDYYNYLVDRYLIAKMIYAEARGESFEGQVAVGAVILNRVKSGIFPNTVAGV
IYQPDAFEPVTNGEFFNHEPDLTAFKAADAALAGWDPTGGALYFFNPATSTSWWIWTRTITNVIGHHWFGI
>Mature_230_residues
TILIEEFIIVIELTPAIAVNGIERRKLMMKNKILKIAVVYLIIFIMIFGGITSAYAYDKIHVVSKGESLYLIAKWYGSDV
NSIKQANGKWSDLIYPGEKLVVPVNENSDYYNYLVDRYLIAKMIYAEARGESFEGQVAVGAVILNRVKSGIFPNTVAGVI
YQPDAFEPVTNGEFFNHEPDLTAFKAADAALAGWDPTGGALYFFNPATSTSWWIWTRTITNVIGHHWFGI

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: NA

Molecular weight: Translated: 25871; Mature: 25740

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILIEEFIIVIELTPAIAVNGIERRKLMMKNKILKIAVVYLIIFIMIFGGITSAYAYDK
CEEEEEEEEEEEEECCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCE
IHVVSKGESLYLIAKWYGSDVNSIKQANGKWSDLIYPGEKLVVPVNENSDYYNYLVDRYL
EEEEECCCEEEEEEEECCCCHHHHHHCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHH
IAKMIYAEARGESFEGQVAVGAVILNRVKSGIFPNTVAGVIYQPDAFEPVTNGEFFNHEP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
DLTAFKAADAALAGWDPTGGALYFFNPATSTSWWIWTRTITNVIGHHWFGI
CCHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TILIEEFIIVIELTPAIAVNGIERRKLMMKNKILKIAVVYLIIFIMIFGGITSAYAYDK
EEEEEEEEEEEEECCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCE
IHVVSKGESLYLIAKWYGSDVNSIKQANGKWSDLIYPGEKLVVPVNENSDYYNYLVDRYL
EEEEECCCEEEEEEEECCCCHHHHHHCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHH
IAKMIYAEARGESFEGQVAVGAVILNRVKSGIFPNTVAGVIYQPDAFEPVTNGEFFNHEP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
DLTAFKAADAALAGWDPTGGALYFFNPATSTSWWIWTRTITNVIGHHWFGI
CCHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]