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The map label for this gene is yjbJ [H]

Identifier: 167040777

GI number: 167040777

Start: 2157909

End: 2158514

Strand: Reverse

Name: yjbJ [H]

Synonym: Teth514_2154

Alternate gene names: 167040777

Gene position: 2158514-2157909 (Counterclockwise)

Preceding gene: 167040778

Following gene: 167040776

Centisome position: 87.84

GC content: 30.03

Gene sequence:

>606_bases
TTGGGGAAGATTTGCAACATAAATCATGATATCGGAGGAGTTACTTTGAGAAAAAAAGTTGCGGTAATATTTTTGATACT
ACTAGGTCTTTTGTTTACTTACGAGTTAAATACTTATTATTTTTTAAAAAAAATTTATCCTCTTAAATACCAGAATTATG
TAGTTTATTACGCAAAAGAGTATGGAGTAGATCCCTATCTTGTTTTTGCCGTAATAAAAGTAGAAAGTAATTTTAAAAGT
AATGCAATTTCCAGTAAAAATGCTATAGGATTAATGCAAATTTTACCAGAGACTGGAGAGTGGATTGCTAAAAAAATAGG
GATAAAAAACTACAGTAATAATATGCTTTTTGAACCTAAATACAATATTCAAATGGGGACATGGTATTTGAGTTATCTTC
TTAAAAATTTCAATGGGAATATGCAGTTGGCTATAGCAGCTTATAACGGAGGTAGTGGAAATGTAGATGCATGGCTTAAA
GACAAAAAATTTTCGAAGGATGGGAAGCAGCTACATGCTGTTCCTTTTCCTGAGACAAATAGATATATAAAAAAGGTGTT
AGCAGTTTATCAGATGTATAAGTTCATATATGAAACTAAAAATTGA

Upstream 100 bases:

>100_bases
TAAAAAGTCAAATGCCTCTGGATGAAAAAATAAGATACGCAGATTTTATAATAAACAATAGTAAGGATTTTAAAGCTATT
GAAAAACAAGTTACACTATT

Downstream 100 bases:

>100_bases
TATGAAAATTAGTATATACAAAAAAAGCTGAAAAATTGGTATAATAGTATCGTTAAAAAAGAAAATAAAAAGGGAGGACA
CAAATGAAACAATTAAAACA

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MGKICNINHDIGGVTLRKKVAVIFLILLGLLFTYELNTYYFLKKIYPLKYQNYVVYYAKEYGVDPYLVFAVIKVESNFKS
NAISSKNAIGLMQILPETGEWIAKKIGIKNYSNNMLFEPKYNIQMGTWYLSYLLKNFNGNMQLAIAAYNGGSGNVDAWLK
DKKFSKDGKQLHAVPFPETNRYIKKVLAVYQMYKFIYETKN

Sequences:

>Translated_201_residues
MGKICNINHDIGGVTLRKKVAVIFLILLGLLFTYELNTYYFLKKIYPLKYQNYVVYYAKEYGVDPYLVFAVIKVESNFKS
NAISSKNAIGLMQILPETGEWIAKKIGIKNYSNNMLFEPKYNIQMGTWYLSYLLKNFNGNMQLAIAAYNGGSGNVDAWLK
DKKFSKDGKQLHAVPFPETNRYIKKVLAVYQMYKFIYETKN
>Mature_200_residues
GKICNINHDIGGVTLRKKVAVIFLILLGLLFTYELNTYYFLKKIYPLKYQNYVVYYAKEYGVDPYLVFAVIKVESNFKSN
AISSKNAIGLMQILPETGEWIAKKIGIKNYSNNMLFEPKYNIQMGTWYLSYLLKNFNGNMQLAIAAYNGGSGNVDAWLKD
KKFSKDGKQLHAVPFPETNRYIKKVLAVYQMYKFIYETKN

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=161, Percent_Identity=32.9192546583851, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082191, Length=172, Percent_Identity=30.8139534883721, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23248; Mature: 23116

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKICNINHDIGGVTLRKKVAVIFLILLGLLFTYELNTYYFLKKIYPLKYQNYVVYYAKE
CCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCEEECCEEEEEEHH
YGVDPYLVFAVIKVESNFKSNAISSKNAIGLMQILPETGEWIAKKIGIKNYSNNMLFEPK
HCCCCEEEEEEEEECCCCCCCCCCCCCCEEHEECCCCCHHHHHHHHCCCCCCCCEEECCC
YNIQMGTWYLSYLLKNFNGNMQLAIAAYNGGSGNVDAWLKDKKFSKDGKQLHAVPFPETN
CCEEEHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCCEEEECCCCCHH
RYIKKVLAVYQMYKFIYETKN
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKICNINHDIGGVTLRKKVAVIFLILLGLLFTYELNTYYFLKKIYPLKYQNYVVYYAKE
CCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCEEECCEEEEEEHH
YGVDPYLVFAVIKVESNFKSNAISSKNAIGLMQILPETGEWIAKKIGIKNYSNNMLFEPK
HCCCCEEEEEEEEECCCCCCCCCCCCCCEEHEECCCCCHHHHHHHHCCCCCCCCEEECCC
YNIQMGTWYLSYLLKNFNGNMQLAIAAYNGGSGNVDAWLKDKKFSKDGKQLHAVPFPETN
CCEEEHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCCEEEECCCCCHH
RYIKKVLAVYQMYKFIYETKN
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]