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The map label for this gene is asnB [H]

Identifier: 167040729

GI number: 167040729

Start: 2114609

End: 2116120

Strand: Reverse

Name: asnB [H]

Synonym: Teth514_2106

Alternate gene names: 167040729

Gene position: 2116120-2114609 (Counterclockwise)

Preceding gene: 167040730

Following gene: 167040727

Centisome position: 86.12

GC content: 32.74

Gene sequence:

>1512_bases
ATGTGCGGATTGGCAGGAATTATAGGAACTGCTGATAAGAGCAAAATACAAAGGATGCTTGAAAAAATACAACATCGTGG
CCCGGATGAGTCAGGTATTTTTGCAGATGAAAATATTACATTAGGCCATAATAGGTTGACGATAATTGACCTTTATCATG
GACGCCAACCTATAAAAAACGAAGATGGCAGGTATTGGCTTATTTATAATGGAGAAATTTATAATTACCAACTATTGCGT
AAAGAGTTAAAAAACCATACTTTTTCCACAGATACTGATAGCGAAGTTATAATTCATTTGTATGAAGAACTAGGTAAAAA
TTGTATTAACTATATTGATGGAATGTTTGCTTTAGCTATTTATGACTCTAAAAAAAAGACCATATTTATTGCTCGCGATC
CGTTAGGAATAAAGCCATTGTATTATGGTAAAACAAAAGAAGGTTATTTTGTCTTTGCTTCGGAGATTAAAGCACTTCAG
GAAGTAACTGATGATATAAATGAATTTCCTAATGGATATATTTATACCAGTGAAAAGGGGTTTGAAAAATATTATTCAAT
TCCCCAGGACCCGATGCATTTTGGAGAGGTTGATGACATTATTAATGGATTAAGGCTTAGATTAGAGGATTCAGTAAGAA
AAAGGTTGATTGCTGATGTGCCTGTTGGAGTATTTTTAAGTGGCGGATTGGATAGCAGCCTTATAGCAGCAATAGCAGCA
AAATACAAAAATCCCCTTCACTCATTTGCAGTAGGTGTCGAAGGAAGTAATGACTTAAAGAATGCAAGAGTTGTTGCTGA
TTATGTAGGTACGATTCACCATGAATTTATTTATACAGAGGAGGACATAAAAAAAATTTTACCCGAAGTTATATATCATC
TTGAATCCTGTGATCCAGCCTTAGTCAGAAGTGCTGTTGCAACATATTTTGTATCAAAACTTGCAAGCAATTACGTAAAG
GTTATTCTTTCAGGAGAAGGTGCTGATGAATTGTTTAGCGGATACCATTACCTTAAAAATTATACCAATTCTTGGAAATT
ACAATCCGAATTAAAATATATAACTCGAAATTTGCATAATACAAATCTTCAAAGAGTTGACAGAATGACAATGGCACATT
CCATTGAGGGACGTGTACCGTTTTTAGATGTAGAAGTGTTACGGTATGCATTTAAAATAACACCGTCATTTAAAATTAAC
GGCCGAGAAAAAATCGAAAAGTGGATACTTAGAAAACTTGCAGAAAATTATTTACCAGAATCAATTGTATGGAGAAGAAA
AGAAAAATTTGCAATAGGAACTGGTACATCTGAAATATTGAATAAGATAGCCGAATCAGAAATTAGCGATGCAGAGTATA
TAAAAAATAGATTCCTACCTAATGGTTTTGAAATAAAGTCTAAAGAAGAGTTGTATTATTTTAAAATTTTAAAAAGATTT
TTTAATGTTGATTCTTTTATAAAGGACATGGGAAGAAGTAGAAGCCTCAATGACAACCAAATATATGCATAA

Upstream 100 bases:

>100_bases
ATCAGCTATAGAAGTATAAAAACTTAGGAACGTAAGATCTTTTCTTACGTTCCTTATTCAATTTCTTTAAATATATCTGA
AGGGGAGGAATGATAGTCAA

Downstream 100 bases:

>100_bases
AAAGAGGGCATTCTGCCCTCTTTTTAAATCAATATTTTGAGAAGATATATTTTTTTCTATTTCTAATTTTCCGTCTTCTC
CAAAGGATAAAAGCTATTCC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MCGLAGIIGTADKSKIQRMLEKIQHRGPDESGIFADENITLGHNRLTIIDLYHGRQPIKNEDGRYWLIYNGEIYNYQLLR
KELKNHTFSTDTDSEVIIHLYEELGKNCINYIDGMFALAIYDSKKKTIFIARDPLGIKPLYYGKTKEGYFVFASEIKALQ
EVTDDINEFPNGYIYTSEKGFEKYYSIPQDPMHFGEVDDIINGLRLRLEDSVRKRLIADVPVGVFLSGGLDSSLIAAIAA
KYKNPLHSFAVGVEGSNDLKNARVVADYVGTIHHEFIYTEEDIKKILPEVIYHLESCDPALVRSAVATYFVSKLASNYVK
VILSGEGADELFSGYHYLKNYTNSWKLQSELKYITRNLHNTNLQRVDRMTMAHSIEGRVPFLDVEVLRYAFKITPSFKIN
GREKIEKWILRKLAENYLPESIVWRRKEKFAIGTGTSEILNKIAESEISDAEYIKNRFLPNGFEIKSKEELYYFKILKRF
FNVDSFIKDMGRSRSLNDNQIYA

Sequences:

>Translated_503_residues
MCGLAGIIGTADKSKIQRMLEKIQHRGPDESGIFADENITLGHNRLTIIDLYHGRQPIKNEDGRYWLIYNGEIYNYQLLR
KELKNHTFSTDTDSEVIIHLYEELGKNCINYIDGMFALAIYDSKKKTIFIARDPLGIKPLYYGKTKEGYFVFASEIKALQ
EVTDDINEFPNGYIYTSEKGFEKYYSIPQDPMHFGEVDDIINGLRLRLEDSVRKRLIADVPVGVFLSGGLDSSLIAAIAA
KYKNPLHSFAVGVEGSNDLKNARVVADYVGTIHHEFIYTEEDIKKILPEVIYHLESCDPALVRSAVATYFVSKLASNYVK
VILSGEGADELFSGYHYLKNYTNSWKLQSELKYITRNLHNTNLQRVDRMTMAHSIEGRVPFLDVEVLRYAFKITPSFKIN
GREKIEKWILRKLAENYLPESIVWRRKEKFAIGTGTSEILNKIAESEISDAEYIKNRFLPNGFEIKSKEELYYFKILKRF
FNVDSFIKDMGRSRSLNDNQIYA
>Mature_503_residues
MCGLAGIIGTADKSKIQRMLEKIQHRGPDESGIFADENITLGHNRLTIIDLYHGRQPIKNEDGRYWLIYNGEIYNYQLLR
KELKNHTFSTDTDSEVIIHLYEELGKNCINYIDGMFALAIYDSKKKTIFIARDPLGIKPLYYGKTKEGYFVFASEIKALQ
EVTDDINEFPNGYIYTSEKGFEKYYSIPQDPMHFGEVDDIINGLRLRLEDSVRKRLIADVPVGVFLSGGLDSSLIAAIAA
KYKNPLHSFAVGVEGSNDLKNARVVADYVGTIHHEFIYTEEDIKKILPEVIYHLESCDPALVRSAVATYFVSKLASNYVK
VILSGEGADELFSGYHYLKNYTNSWKLQSELKYITRNLHNTNLQRVDRMTMAHSIEGRVPFLDVEVLRYAFKITPSFKIN
GREKIEKWILRKLAENYLPESIVWRRKEKFAIGTGTSEILNKIAESEISDAEYIKNRFLPNGFEIKSKEELYYFKILKRF
FNVDSFIKDMGRSRSLNDNQIYA

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=531, Percent_Identity=34.0866290018832, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI168229252, Length=531, Percent_Identity=34.0866290018832, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI168229250, Length=531, Percent_Identity=34.0866290018832, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI296010848, Length=509, Percent_Identity=34.3811394891945, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI296010852, Length=439, Percent_Identity=33.9407744874715, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI296010850, Length=439, Percent_Identity=33.9407744874715, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI1786889, Length=512, Percent_Identity=40.234375, Blast_Score=350, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI25147557, Length=517, Percent_Identity=38.4912959381045, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI71993933, Length=517, Percent_Identity=38.4912959381045, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17560178, Length=515, Percent_Identity=35.3398058252427, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI25147560, Length=305, Percent_Identity=40, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321563, Length=536, Percent_Identity=38.8059701492537, Blast_Score=332, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6325403, Length=537, Percent_Identity=37.4301675977654, Blast_Score=323, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6323542, Length=479, Percent_Identity=25.2609603340292, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45553209, Length=489, Percent_Identity=36.1963190184049, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 57898; Mature: 57898

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLAGIIGTADKSKIQRMLEKIQHRGPDESGIFADENITLGHNRLTIIDLYHGRQPIKN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCEEECCCCEEEEEEECCCCCCCC
EDGRYWLIYNGEIYNYQLLRKELKNHTFSTDTDSEVIIHLYEELGKNCINYIDGMFALAI
CCCCEEEEECCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
YDSKKKTIFIARDPLGIKPLYYGKTKEGYFVFASEIKALQEVTDDINEFPNGYIYTSEKG
EECCCCEEEEEECCCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCC
FEKYYSIPQDPMHFGEVDDIINGLRLRLEDSVRKRLIADVPVGVFLSGGLDSSLIAAIAA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHH
KYKNPLHSFAVGVEGSNDLKNARVVADYVGTIHHEFIYTEEDIKKILPEVIYHLESCDPA
HHCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHCCCHH
LVRSAVATYFVSKLASNYVKVILSGEGADELFSGYHYLKNYTNSWKLQSELKYITRNLHN
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
TNLQRVDRMTMAHSIEGRVPFLDVEVLRYAFKITPSFKINGREKIEKWILRKLAENYLPE
CCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCH
SIVWRRKEKFAIGTGTSEILNKIAESEISDAEYIKNRFLPNGFEIKSKEELYYFKILKRF
HHHHHHHHCEEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
FNVDSFIKDMGRSRSLNDNQIYA
HCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MCGLAGIIGTADKSKIQRMLEKIQHRGPDESGIFADENITLGHNRLTIIDLYHGRQPIKN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCEEECCCCEEEEEEECCCCCCCC
EDGRYWLIYNGEIYNYQLLRKELKNHTFSTDTDSEVIIHLYEELGKNCINYIDGMFALAI
CCCCEEEEECCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
YDSKKKTIFIARDPLGIKPLYYGKTKEGYFVFASEIKALQEVTDDINEFPNGYIYTSEKG
EECCCCEEEEEECCCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCC
FEKYYSIPQDPMHFGEVDDIINGLRLRLEDSVRKRLIADVPVGVFLSGGLDSSLIAAIAA
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHH
KYKNPLHSFAVGVEGSNDLKNARVVADYVGTIHHEFIYTEEDIKKILPEVIYHLESCDPA
HHCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHCCCHH
LVRSAVATYFVSKLASNYVKVILSGEGADELFSGYHYLKNYTNSWKLQSELKYITRNLHN
HHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
TNLQRVDRMTMAHSIEGRVPFLDVEVLRYAFKITPSFKINGREKIEKWILRKLAENYLPE
CCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCH
SIVWRRKEKFAIGTGTSEILNKIAESEISDAEYIKNRFLPNGFEIKSKEELYYFKILKRF
HHHHHHHHCEEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
FNVDSFIKDMGRSRSLNDNQIYA
HCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]