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The map label for this gene is gltX [H]

Identifier: 167040725

GI number: 167040725

Start: 2110499

End: 2111938

Strand: Reverse

Name: gltX [H]

Synonym: Teth514_2102

Alternate gene names: 167040725

Gene position: 2111938-2110499 (Counterclockwise)

Preceding gene: 167040727

Following gene: 167040724

Centisome position: 85.95

GC content: 34.86

Gene sequence:

>1440_bases
ATGAGTGAAGTGAGAGTAAGATTTGCCCCAAGTCCTACAGGGAGTTTACATATAGGTGGTGCCAGGACTGCTCTTTTTAA
TTGGCTTTTTGCAAGGCACAATGGAGGAAAATTTATTTTAAGGGTGGATGACACTGACCTACAAAGGTCGACAGAAGAAT
CTATGAAAGGGATACTTGAAGGACTGAAGTGGTTAGGTATTGATTGGGATGAAGGACCTATTTACCAATCGCAAAGGTTA
GAAGAATATAGAAAATTCGCAAATAAATTATTAAAGGAAGGAAAGGCTTACTACTGTTTTTGCACAAAAGAAGAGTTAGA
GGAAATGAGAAAACAGGCAGAAAAAGAAGGTAGGCCTCCAATGTATACGGGAAAATGTAGAAATTTGACCAAAGAGCAAA
TAGAGGAGTATTTAAGGCAAGGGAAAAAACCTGTAATAAGATTAAAAGTGCCTCAACAAGGGAAGACAGTGGTTCACGAC
ATAATAAGAGGGGATGTAGAGTTTGATAATTCCACCTTTGATGACTTTATAATTATGAAATCAGACAATATGCCTACTTA
TAATTTTGCTACTGTGGTAGATGATTACCAAATGGGGATAACACATGTTATACGGGCAGAGGAACATTTGTCAAATACCC
CAAAGCAAATACTAATTTTTGAAGCTTTAGGCTTAGAAATTCCTCAATTTGCCCATGTGTCTATGGTTTTAGCCCCTGAT
AGAAGTAAACTTAGTAAAAGACATGGAGCGACTTCTGTGCAGGAGTTTAGAGACCAAGGATATCTTCCTGAAGCAATTGT
AAATTATATAACATTGCTGGGATGGATTCCAAAAGATGGGGAAGAAATTTTTGATGTGTCAAAAAGCATAAAAGAATTTA
CTTTAGAAAGGGTATCAAAAAATCCAGCTATTTACGATGTACAAAAGCTGACGTGGATAAACGGCCATTACATAAGGAAT
TATGATTTAGATAAATTAACTGAAGTTGTCATTCCATTTTTGAAAGCTAAAAACTTTATTGGGGAAGATTTCGATTATGA
TTATATCAAAAAAATAGTAAGTGTAGTAAGAGAAAGGGAAAAAACTCTTGTGGATGTAGCTGATGCCATGAGCTATTATT
TTACAGAAGTTAACGAATATGAGGAAAAAGGAGTTAAAAAGTATTTTACTAAAGAAAAAGTTGTGGATATCCTAAAAAAA
GCAGTAGTGACTTTAAAAGAAGTCGAGCCTTTTAATAAATTTACAACTGAGGAAACGTATCGCAAACTTGTGGAGGAATT
ACAAATATCCAGTGGTGAACTTTTCCATCCCACGAGATTAGCTATTTCAGGTAGAACTTTTGGACCAGGACTATTTGACA
TAATGGAACTTTTGGGAAAAGAAAGAACTATAAAGAGAATTGAGAAAGCCATAGATTTTATTCAAAAGATGAGGAAATAA

Upstream 100 bases:

>100_bases
ATGATTATACCACAAATTTTTGATGAAATACTTCTTTGTTGAAAAAACACTTCAATTAAAGTATAATCAAAATAGTTTAA
ACTTATGAAGAGGTGATAAG

Downstream 100 bases:

>100_bases
CAAAAATATTGACATAGTGTATAATTTTTACTACTATGATAATAGTATTACCCTTATAAGGGTGATACTATTATTTTAAC
CCATGAAAGGGTGATACCAC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2 [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSEVRVRFAPSPTGSLHIGGARTALFNWLFARHNGGKFILRVDDTDLQRSTEESMKGILEGLKWLGIDWDEGPIYQSQRL
EEYRKFANKLLKEGKAYYCFCTKEELEEMRKQAEKEGRPPMYTGKCRNLTKEQIEEYLRQGKKPVIRLKVPQQGKTVVHD
IIRGDVEFDNSTFDDFIIMKSDNMPTYNFATVVDDYQMGITHVIRAEEHLSNTPKQILIFEALGLEIPQFAHVSMVLAPD
RSKLSKRHGATSVQEFRDQGYLPEAIVNYITLLGWIPKDGEEIFDVSKSIKEFTLERVSKNPAIYDVQKLTWINGHYIRN
YDLDKLTEVVIPFLKAKNFIGEDFDYDYIKKIVSVVREREKTLVDVADAMSYYFTEVNEYEEKGVKKYFTKEKVVDILKK
AVVTLKEVEPFNKFTTEETYRKLVEELQISSGELFHPTRLAISGRTFGPGLFDIMELLGKERTIKRIEKAIDFIQKMRK

Sequences:

>Translated_479_residues
MSEVRVRFAPSPTGSLHIGGARTALFNWLFARHNGGKFILRVDDTDLQRSTEESMKGILEGLKWLGIDWDEGPIYQSQRL
EEYRKFANKLLKEGKAYYCFCTKEELEEMRKQAEKEGRPPMYTGKCRNLTKEQIEEYLRQGKKPVIRLKVPQQGKTVVHD
IIRGDVEFDNSTFDDFIIMKSDNMPTYNFATVVDDYQMGITHVIRAEEHLSNTPKQILIFEALGLEIPQFAHVSMVLAPD
RSKLSKRHGATSVQEFRDQGYLPEAIVNYITLLGWIPKDGEEIFDVSKSIKEFTLERVSKNPAIYDVQKLTWINGHYIRN
YDLDKLTEVVIPFLKAKNFIGEDFDYDYIKKIVSVVREREKTLVDVADAMSYYFTEVNEYEEKGVKKYFTKEKVVDILKK
AVVTLKEVEPFNKFTTEETYRKLVEELQISSGELFHPTRLAISGRTFGPGLFDIMELLGKERTIKRIEKAIDFIQKMRK
>Mature_478_residues
SEVRVRFAPSPTGSLHIGGARTALFNWLFARHNGGKFILRVDDTDLQRSTEESMKGILEGLKWLGIDWDEGPIYQSQRLE
EYRKFANKLLKEGKAYYCFCTKEELEEMRKQAEKEGRPPMYTGKCRNLTKEQIEEYLRQGKKPVIRLKVPQQGKTVVHDI
IRGDVEFDNSTFDDFIIMKSDNMPTYNFATVVDDYQMGITHVIRAEEHLSNTPKQILIFEALGLEIPQFAHVSMVLAPDR
SKLSKRHGATSVQEFRDQGYLPEAIVNYITLLGWIPKDGEEIFDVSKSIKEFTLERVSKNPAIYDVQKLTWINGHYIRNY
DLDKLTEVVIPFLKAKNFIGEDFDYDYIKKIVSVVREREKTLVDVADAMSYYFTEVNEYEEKGVKKYFTKEKVVDILKKA
VVTLKEVEPFNKFTTEETYRKLVEELQISSGELFHPTRLAISGRTFGPGLFDIMELLGKERTIKRIEKAIDFIQKMRK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=33.469387755102, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI62241042, Length=253, Percent_Identity=31.2252964426877, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI4826960, Length=296, Percent_Identity=26.6891891891892, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788743, Length=477, Percent_Identity=40.461215932914, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI226510925, Length=248, Percent_Identity=32.6612903225806, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1786895, Length=335, Percent_Identity=23.5820895522388, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=34.0248962655602, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17511007, Length=282, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=233, Percent_Identity=26.6094420600858, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=509, Percent_Identity=34.7740667976424, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321192, Length=253, Percent_Identity=31.6205533596838, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324742, Length=310, Percent_Identity=28.7096774193548, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=34.0080971659919, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI17864488, Length=308, Percent_Identity=26.9480519480519, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24649466, Length=262, Percent_Identity=27.0992366412214, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55662; Mature: 55530

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00050 RIBOSOMAL_L23 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVRVRFAPSPTGSLHIGGARTALFNWLFARHNGGKFILRVDDTDLQRSTEESMKGILE
CCCCEEEECCCCCCCEEECHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHH
GLKWLGIDWDEGPIYQSQRLEEYRKFANKLLKEGKAYYCFCTKEELEEMRKQAEKEGRPP
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCC
MYTGKCRNLTKEQIEEYLRQGKKPVIRLKVPQQGKTVVHDIIRGDVEFDNSTFDDFIIMK
CCCCHHHCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEEE
SDNMPTYNFATVVDDYQMGITHVIRAEEHLSNTPKQILIFEALGLEIPQFAHVSMVLAPD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEHHHCCCCCCHHCEEEEECCC
RSKLSKRHGATSVQEFRDQGYLPEAIVNYITLLGWIPKDGEEIFDVSKSIKEFTLERVSK
HHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
NPAIYDVQKLTWINGHYIRNYDLDKLTEVVIPFLKAKNFIGEDFDYDYIKKIVSVVRERE
CCCEEEHHHEEEECCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KTLVDVADAMSYYFTEVNEYEEKGVKKYFTKEKVVDILKKAVVTLKEVEPFNKFTTEETY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHH
RKLVEELQISSGELFHPTRLAISGRTFGPGLFDIMELLGKERTIKRIEKAIDFIQKMRK
HHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEVRVRFAPSPTGSLHIGGARTALFNWLFARHNGGKFILRVDDTDLQRSTEESMKGILE
CCCEEEECCCCCCCEEECHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHH
GLKWLGIDWDEGPIYQSQRLEEYRKFANKLLKEGKAYYCFCTKEELEEMRKQAEKEGRPP
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCC
MYTGKCRNLTKEQIEEYLRQGKKPVIRLKVPQQGKTVVHDIIRGDVEFDNSTFDDFIIMK
CCCCHHHCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEEEE
SDNMPTYNFATVVDDYQMGITHVIRAEEHLSNTPKQILIFEALGLEIPQFAHVSMVLAPD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEHHHCCCCCCHHCEEEEECCC
RSKLSKRHGATSVQEFRDQGYLPEAIVNYITLLGWIPKDGEEIFDVSKSIKEFTLERVSK
HHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
NPAIYDVQKLTWINGHYIRNYDLDKLTEVVIPFLKAKNFIGEDFDYDYIKKIVSVVRERE
CCCEEEHHHEEEECCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KTLVDVADAMSYYFTEVNEYEEKGVKKYFTKEKVVDILKKAVVTLKEVEPFNKFTTEETY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHH
RKLVEELQISSGELFHPTRLAISGRTFGPGLFDIMELLGKERTIKRIEKAIDFIQKMRK
HHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA