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The map label for this gene is rlmN

Identifier: 167040388

GI number: 167040388

Start: 1754655

End: 1755683

Strand: Reverse

Name: rlmN

Synonym: Teth514_1751

Alternate gene names: 167040388

Gene position: 1755683-1754655 (Counterclockwise)

Preceding gene: 167040389

Following gene: 167040387

Centisome position: 71.45

GC content: 33.14

Gene sequence:

>1029_bases
TTGTACAATTTAAAAGACATGACATTAGAAGAAATGGAAGAATTCTTTGTAAATATTGGAGAGAGTAGATATAGAGCTAA
GCAGATTTACAAGTGGATTTATGGCAAAAAAGTGACAGATTTTGATCAGATGACAGATATCTCTAAGAATTTGCGCTCAA
AATTAAAAGAAATTGCTTATGTTTCCCAATTAAAAATTGAAGAGAGAAGGGTTTCTGAGATAGATGACACAGTAAAGTAC
CTTTTTTTACTAGAAGACGGTAATATCATCGAAGGAGTTGCCATAAAATACAGATTTGGAAATACTGCTTGTGTTTCTAC
TCAAGTAGGATGCAATATGAGATGTAGCTTTTGCGCTTCTGCTATTGGAGGTAAAGTTAGAGATTTAAAAGCCTCAGAGA
TGGTAGACCAAGTTATGGCTATAGACAGCGACTATGGCAAGATTTCTAATATTGTTTTGATGGGAAGTGGGGAACCTTTT
GACAATTATGATGAAGTGATGAAATTTATAAAAATAGTGAATAACCCCCACGGTTTAGGAATAGGCAGCAGACATATAAC
TATATCTACTTGTGGAATAGTTCCTAAAATTTATCAATTTGCAGATGAAAAACTACAGGTGAATTTATCTATTTCATTAC
ATGCTCCTAATGATGAATTGAGAACTCAACTAATGCCAATAAATAAAGCTTATCCATTAGAGGAATTGATGAAGGCTTGT
AAATATTATGTAGATAAAACAAGGCGCAGGATAACCTTTGAGTATTCGTTGATTGAAGGAGTAAATGATAAAAAAGAACA
TGCGTATCAATTGGTTGATTTATTAAAAGGTATGTTGTGTCATATTAATTTAATACCTATTAATTACGTAAGAGAAATAG
GTTTTAAAAAGGCAAACAATGAAAAGGTGATGATGTTCAAAAGAATTATTGAAGATGCAGGGATATCTTGTACGGTGAGA
AGGGAATTGGGTAGCGATATTGAAGCTGCCTGTGGACAGCTGAGGAGAAAGTATTTAAAGGAAAGGTAG

Upstream 100 bases:

>100_bases
TAGGGGAGTTAGTCTGTGCTAAATGTCAGCTAAATAAGTTACCTACTGATTCTTGACAGGGACAAATCTTAATCTTAAAT
GAACACGGAGGTAATTTGAG

Downstream 100 bases:

>100_bases
GGTTTTTATGATTGCAGCTGCCCTAACTGATATTGGCAATGTAAGAGAAAAAAATGAAGATTATTATTATGCTTCGGAAG
AAAAAGATTTGCCACTGTTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MYNLKDMTLEEMEEFFVNIGESRYRAKQIYKWIYGKKVTDFDQMTDISKNLRSKLKEIAYVSQLKIEERRVSEIDDTVKY
LFLLEDGNIIEGVAIKYRFGNTACVSTQVGCNMRCSFCASAIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPF
DNYDEVMKFIKIVNNPHGLGIGSRHITISTCGIVPKIYQFADEKLQVNLSISLHAPNDELRTQLMPINKAYPLEELMKAC
KYYVDKTRRRITFEYSLIEGVNDKKEHAYQLVDLLKGMLCHINLIPINYVREIGFKKANNEKVMMFKRIIEDAGISCTVR
RELGSDIEAACGQLRRKYLKER

Sequences:

>Translated_342_residues
MYNLKDMTLEEMEEFFVNIGESRYRAKQIYKWIYGKKVTDFDQMTDISKNLRSKLKEIAYVSQLKIEERRVSEIDDTVKY
LFLLEDGNIIEGVAIKYRFGNTACVSTQVGCNMRCSFCASAIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPF
DNYDEVMKFIKIVNNPHGLGIGSRHITISTCGIVPKIYQFADEKLQVNLSISLHAPNDELRTQLMPINKAYPLEELMKAC
KYYVDKTRRRITFEYSLIEGVNDKKEHAYQLVDLLKGMLCHINLIPINYVREIGFKKANNEKVMMFKRIIEDAGISCTVR
RELGSDIEAACGQLRRKYLKER
>Mature_342_residues
MYNLKDMTLEEMEEFFVNIGESRYRAKQIYKWIYGKKVTDFDQMTDISKNLRSKLKEIAYVSQLKIEERRVSEIDDTVKY
LFLLEDGNIIEGVAIKYRFGNTACVSTQVGCNMRCSFCASAIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPF
DNYDEVMKFIKIVNNPHGLGIGSRHITISTCGIVPKIYQFADEKLQVNLSISLHAPNDELRTQLMPINKAYPLEELMKAC
KYYVDKTRRRITFEYSLIEGVNDKKEHAYQLVDLLKGMLCHINLIPINYVREIGFKKANNEKVMMFKRIIEDAGISCTVR
RELGSDIEAACGQLRRKYLKER

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=40.1162790697674, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_THEP3 (B0KA06)

Other databases:

- EMBL:   CP000924
- RefSeq:   YP_001665305.1
- ProteinModelPortal:   B0KA06
- GeneID:   5875089
- GenomeReviews:   CP000924_GR
- KEGG:   tpd:Teth39_1315
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14454
- BioCyc:   TPSE340099:TETH39_1315-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39340; Mature: 39340

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLKDMTLEEMEEFFVNIGESRYRAKQIYKWIYGKKVTDFDQMTDISKNLRSKLKEIAY
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VSQLKIEERRVSEIDDTVKYLFLLEDGNIIEGVAIKYRFGNTACVSTQVGCNMRCSFCAS
HHHHHHHHHHHHHHHHHEEEEEEECCCCEEECEEEEEECCCCEEEEECCCCCCHHHHHHH
AIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPFDNYDEVMKFIKIVNNPHGLG
HHCCHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
IGSRHITISTCGIVPKIYQFADEKLQVNLSISLHAPNDELRTQLMPINKAYPLEELMKAC
CCCCEEEEEECCCHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHH
KYYVDKTRRRITFEYSLIEGVNDKKEHAYQLVDLLKGMLCHINLIPINYVREIGFKKANN
HHHHHHHHHEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHCCCCCCC
EKVMMFKRIIEDAGISCTVRRELGSDIEAACGQLRRKYLKER
CHHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYNLKDMTLEEMEEFFVNIGESRYRAKQIYKWIYGKKVTDFDQMTDISKNLRSKLKEIAY
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VSQLKIEERRVSEIDDTVKYLFLLEDGNIIEGVAIKYRFGNTACVSTQVGCNMRCSFCAS
HHHHHHHHHHHHHHHHHEEEEEEECCCCEEECEEEEEECCCCEEEEECCCCCCHHHHHHH
AIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPFDNYDEVMKFIKIVNNPHGLG
HHCCHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
IGSRHITISTCGIVPKIYQFADEKLQVNLSISLHAPNDELRTQLMPINKAYPLEELMKAC
CCCCEEEEEECCCHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHH
KYYVDKTRRRITFEYSLIEGVNDKKEHAYQLVDLLKGMLCHINLIPINYVREIGFKKANN
HHHHHHHHHEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHCCCCCCC
EKVMMFKRIIEDAGISCTVRRELGSDIEAACGQLRRKYLKER
CHHHHHHHHHHHCCCEEEEHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA