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The map label for this gene is ispG [H]

Identifier: 167040289

GI number: 167040289

Start: 1670754

End: 1671815

Strand: Reverse

Name: ispG [H]

Synonym: Teth514_1652

Alternate gene names: 167040289

Gene position: 1671815-1670754 (Counterclockwise)

Preceding gene: 167040290

Following gene: 167040288

Centisome position: 68.04

GC content: 33.43

Gene sequence:

>1062_bases
ATGAGAAAAATTACTCGCGAAGTCAAAATAGGCAATAAAAAAATTGGAGGAAATAATCCAATTTTAGTTCAGTCTATGAC
TAACACGGATACCCACGATATTGAAAAAACAATAGAGCAGATAAAAAGACTAGAAGCTGAAGGCTGTGATATTATAAGAG
TTGCTGTTCCAGATATGGAAGCAGCTGAAGCAATAAAAGAAATAAAGAAAAATATAAATATTCCTTTGGTAGCAGATATT
CATTTTGATTATCGATTAGCTATAAAATCAATTGAAAATGGAGCAGACAAGATAAGAATTAATCCCGGAAACATAGGAAG
AGAAGAAAATATCAAAAAAGTGGTAGAAATAGCAAAAGAAAAAGGTATTCCTATTAGAATAGGTGTAAATTCTGGTTCTT
TAGAAAAAGAAATATTACATAAATACAAGGGAGTAACTGCCGAAGCAGTTGTGGAAAGCGCTTTAAAAAATGTTTTAATT
TTAGAAAAGTTAGGCTTTTATGATATAGTTATTTCGCTTAAAACTACAAATGTACCCCTTACTATAGAGGCTTATAAACT
CGCTTCTTCAAAAGTGGATTATCCATTGCACGTGGGTATAACGGAAGCGGGTACTATTGAAGCCGGTACTATAAAATCTG
CTATAGGAATAGGAACATTACTTTATTTAGGAATAGGAGACACAATACGAGTTTCTTTAACGGGTGATCCAGTCCATGAG
GTGAGAGTAGGAAGACAGATTTTACGTTCTTTAGGTCTTTTAAAAGAAGGAGTCGAAATCATTTCCTGCCCTACCTGCGG
CAGAACAAAAATTGACTTGATAAGGTTAGCAGAAGAAGTAGAAAAAAGGACGAGGAATATTAAAAAGCCCTTAAAAGTTG
CTGTTATGGGATGTGCTGTAAATGGACCAGGGGAAGCAAAAGAAGCAGATATAGGAATAGCAGGAGGAGATGGAGAAGGA
GTTATTTTTAAAAAAGGAAAAGTTTATAAAAAAGTTAAAGAAGAAGAGTTAGTAGAAGAATTGATGAAAGAAATTGAAAA
ATTACTTGAGGAGGATTCTTAA

Upstream 100 bases:

>100_bases
AGAAGGCTATATTCATTATTTAGGTTTTTTACTGTTGATTGCTTTGTTGATTTTTGCAACCTATAGGGATATTCTACGCA
TTTTTTAAAAGGTGATAAAA

Downstream 100 bases:

>100_bases
TGATTTCTGTTGTAATCCCAGCTTTTAATGAAGGAAAAAATATTGGGAGAGTTTTGTCAGTCTTAGAAACAATAGACCTG
ATTGATGAAATCATAGTTGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MRKITREVKIGNKKIGGNNPILVQSMTNTDTHDIEKTIEQIKRLEAEGCDIIRVAVPDMEAAEAIKEIKKNINIPLVADI
HFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEIAKEKGIPIRIGVNSGSLEKEILHKYKGVTAEAVVESALKNVLI
LEKLGFYDIVISLKTTNVPLTIEAYKLASSKVDYPLHVGITEAGTIEAGTIKSAIGIGTLLYLGIGDTIRVSLTGDPVHE
VRVGRQILRSLGLLKEGVEIISCPTCGRTKIDLIRLAEEVEKRTRNIKKPLKVAVMGCAVNGPGEAKEADIGIAGGDGEG
VIFKKGKVYKKVKEEELVEELMKEIEKLLEEDS

Sequences:

>Translated_353_residues
MRKITREVKIGNKKIGGNNPILVQSMTNTDTHDIEKTIEQIKRLEAEGCDIIRVAVPDMEAAEAIKEIKKNINIPLVADI
HFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEIAKEKGIPIRIGVNSGSLEKEILHKYKGVTAEAVVESALKNVLI
LEKLGFYDIVISLKTTNVPLTIEAYKLASSKVDYPLHVGITEAGTIEAGTIKSAIGIGTLLYLGIGDTIRVSLTGDPVHE
VRVGRQILRSLGLLKEGVEIISCPTCGRTKIDLIRLAEEVEKRTRNIKKPLKVAVMGCAVNGPGEAKEADIGIAGGDGEG
VIFKKGKVYKKVKEEELVEELMKEIEKLLEEDS
>Mature_353_residues
MRKITREVKIGNKKIGGNNPILVQSMTNTDTHDIEKTIEQIKRLEAEGCDIIRVAVPDMEAAEAIKEIKKNINIPLVADI
HFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEIAKEKGIPIRIGVNSGSLEKEILHKYKGVTAEAVVESALKNVLI
LEKLGFYDIVISLKTTNVPLTIEAYKLASSKVDYPLHVGITEAGTIEAGTIKSAIGIGTLLYLGIGDTIRVSLTGDPVHE
VRVGRQILRSLGLLKEGVEIISCPTCGRTKIDLIRLAEEVEKRTRNIKKPLKVAVMGCAVNGPGEAKEADIGIAGGDGEG
VIFKKGKVYKKVKEEELVEELMKEIEKLLEEDS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=344, Percent_Identity=49.7093023255814, Blast_Score=350, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 38612; Mature: 38612

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50290 PI3_4_KINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKITREVKIGNKKIGGNNPILVQSMTNTDTHDIEKTIEQIKRLEAEGCDIIRVAVPDME
CCCCHHHHCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
AAEAIKEIKKNINIPLVADIHFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEIAKE
HHHHHHHHHHCCCCCEEEEECCCCEEEEHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHH
KGIPIRIGVNSGSLEKEILHKYKGVTAEAVVESALKNVLILEKLGFYDIVISLKTTNVPL
CCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCE
TIEAYKLASSKVDYPLHVGITEAGTIEAGTIKSAIGIGTLLYLGIGDTIRVSLTGDPVHE
EEEEEEHHCCCCCCCEEECCCCCCCEECCHHHHHHHHHHHEEEECCCEEEEEECCCCHHH
VRVGRQILRSLGLLKEGVEIISCPTCGRTKIDLIRLAEEVEKRTRNIKKPLKVAVMGCAV
HHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
NGPGEAKEADIGIAGGDGEGVIFKKGKVYKKVKEEELVEELMKEIEKLLEEDS
CCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKITREVKIGNKKIGGNNPILVQSMTNTDTHDIEKTIEQIKRLEAEGCDIIRVAVPDME
CCCCHHHHCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
AAEAIKEIKKNINIPLVADIHFDYRLAIKSIENGADKIRINPGNIGREENIKKVVEIAKE
HHHHHHHHHHCCCCCEEEEECCCCEEEEHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHH
KGIPIRIGVNSGSLEKEILHKYKGVTAEAVVESALKNVLILEKLGFYDIVISLKTTNVPL
CCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCE
TIEAYKLASSKVDYPLHVGITEAGTIEAGTIKSAIGIGTLLYLGIGDTIRVSLTGDPVHE
EEEEEEHHCCCCCCCEEECCCCCCCEECCHHHHHHHHHHHEEEECCCEEEEEECCCCHHH
VRVGRQILRSLGLLKEGVEIISCPTCGRTKIDLIRLAEEVEKRTRNIKKPLKVAVMGCAV
HHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
NGPGEAKEADIGIAGGDGEGVIFKKGKVYKKVKEEELVEELMKEIEKLLEEDS
CCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]