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The map label for this gene is pnp [H]

Identifier: 167040276

GI number: 167040276

Start: 1655922

End: 1658024

Strand: Reverse

Name: pnp [H]

Synonym: Teth514_1639

Alternate gene names: 167040276

Gene position: 1658024-1655922 (Counterclockwise)

Preceding gene: 167040277

Following gene: 167040275

Centisome position: 67.47

GC content: 37.33

Gene sequence:

>2103_bases
ATGGAAGAACGAACTTTTGAAATGGAACTAGCCGGGAGAAAGTTGTTAGTCCAGATAGGAAAAGTAGCTCAACAAGCAAA
TGGAGCAGCTTGGGTAAAATACGGGGATACTGTCGTTCTAGTTACTGCCTGTGCTTCCAAAGAACCCCGAGAAGGAATTG
ACTTTTTTCCTTTAACTGTTGAGTACGAAGAAAGATTGTACTCTGTCGGTAAAATTCCTGGTGGTTTTATAAAAAGAGAA
GGAAAACCTAGTGAAAAGGCAATTCTCTCTGCTCGGTTAATTGACAGACCAATTAGGCCTTTATTTCCCCATGGTTATAG
AAATGACGTACAAGTTATAGCTACAGTTTTATCTGTTGATCCTGATGTACAACCAGAAATAGTCGCAATGATTGGTTCTT
CGGTTGCTCTTTCAATTTCTGATATTCCTTTTGATGGTCCTACAGGTGCGGTAGCAGTAGGATTAGTCGATGGACAATTT
ATAATTAATCCTAACCATGAGCAAAGAGAAAAAAGCTTAATGCATTTAGTGGTTTCAGGAACGAAAGATGCTATTGTAAT
GGTGGAAGCAGGAGCAAAAGAGGTTCCTGAAGAAACTATGCTAGATGCTATAATGTATGCTCACGAATATATCAAACAGA
TTGTAGAGTTTATAGAAGGAATAGTTAAAGAAGTAGGTGTTCCGAAAAGCGAAGTAATTTTACATGAAATAGACAAAGAA
CTAGAAGAAAAAGTAAGAGCCTATGCAACAGAAAAAATATACAATGCGTTAAGAACAGCAGAAAAGAAAGAGAGAAACGA
CAACTTAGATAAAGTTGAACAAGAAGTTTTAGAGCACTTTAAAGAAGAATATCCTGATAATCTTGCTGATATAGATGAAG
TACTTTACAATATAATGAAAGAACAAATGCGAAAAATGATAACAGAGGAAAGAATAAGAGTAGATGGCAGGGGCCTTGAC
GACATAAGACCAATATGGTGCGAAGTAGGAGTGCTACCCAGAACTCACGGTTCTGCGATTTTCACAAGAGGACAAACTCA
AGTGCTTACAGTAGCAACATTAGGTGCATTAGGAGATATTCAGATTTTAGATGGCATAGGAGATGAAGAAGCAAAAAGAT
ATATGCATCATTACAATTTTCCACCCTACAGTGTTGGAGAAGTAAGGCCTCTAAGAGGTCCTGGTAGGAGGGAAATTGGT
CATGGAGCTTTAGCAGAAAGGGCTTTAGAACCGGTGATTCCTTCTGAAGAGGAATTTCCCTATACTATAAGATTAGTGTC
AGAAGTTTTAAGTTCCAATGGTTCTACTTCTCAAGCTAGTGTTTGTGGCAGTACTTTAGCTTTAATGGATGCAGGAGTTC
CAATAAAAGCTCCAGTCGCAGGAGTTGCTATGGGGCTTATCAAAGAAGGAGATGTAGTTTCTGTACTCACTGATATACAA
GGAATTGAAGACTTTTTAGGGGATATGGACTTCAAAGTAGCAGGCACAGAAAAAGGAATCACAGCGATACAAATGGATAT
AAAAATTCCTGGAATTGACAAAGAAATTTTAAAAATGGCTTTAGAAAAGGCCCGAAGGGGTAGACTATACATTCTCAGCA
AAATGTTAGAAGTTATAAAAGAACCGAGAAAACAGTTATCAGTATACGCACCTCGTGTTATAAGAATGGTAGTTGACCCT
GAAAAAATTAGAGAAATAATCGGACCTGGCGGCAAAACCATAAGTAAAATTATTGCAGAAACGGGAGTAAAAATTGATAT
TGAAGAAGACGGCAGGTTATACATTACTGCTTCTGATTTGAGATCTGGCGAAAGAGCTAAGCAAATGATTGAGGCTATAA
CAAAAGATATAGCAGTAGGAGAAATTTATTTAGGAAAAGTGCTTAGAATAACACCCTTTGGAGCTTTTGTAGAAATTGCT
CCTGGTAAAGAAGGACTTGTCCATATTTCTAAGCTATCGAAAAAACGGGTACAAAAAGTAGAAGATGTAGTAAAAGTTGG
CGATGACATATTGGTAAAAGTCACAGATATAGATAAACTAGGAAGAATAAGTCTTTCAAGAAAAGACGCATTGCCTGATG
AAGAGGAAGAAGAAAGAAACTAA

Upstream 100 bases:

>100_bases
TATGGAAAGGTATCGTGCCATTATCGAAAAATTAGATTTAAGAAAATAGAGCGAGATACCTCGCTCTATTTTCTATAAAA
TTTTACGAAAGGAGGATAAC

Downstream 100 bases:

>100_bases
AGGCATAAACCGACCCACGGTTTATGTTTTTTATTTTTTAGTAATATATTCGCCGCCTGTTTGAATAAAATTAAAGTGAG
AATATCAACGGGTGGTGTTA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MEERTFEMELAGRKLLVQIGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTVEYEERLYSVGKIPGGFIKRE
GKPSEKAILSARLIDRPIRPLFPHGYRNDVQVIATVLSVDPDVQPEIVAMIGSSVALSISDIPFDGPTGAVAVGLVDGQF
IINPNHEQREKSLMHLVVSGTKDAIVMVEAGAKEVPEETMLDAIMYAHEYIKQIVEFIEGIVKEVGVPKSEVILHEIDKE
LEEKVRAYATEKIYNALRTAEKKERNDNLDKVEQEVLEHFKEEYPDNLADIDEVLYNIMKEQMRKMITEERIRVDGRGLD
DIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGALGDIQILDGIGDEEAKRYMHHYNFPPYSVGEVRPLRGPGRREIG
HGALAERALEPVIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGVAMGLIKEGDVVSVLTDIQ
GIEDFLGDMDFKVAGTEKGITAIQMDIKIPGIDKEILKMALEKARRGRLYILSKMLEVIKEPRKQLSVYAPRVIRMVVDP
EKIREIIGPGGKTISKIIAETGVKIDIEEDGRLYITASDLRSGERAKQMIEAITKDIAVGEIYLGKVLRITPFGAFVEIA
PGKEGLVHISKLSKKRVQKVEDVVKVGDDILVKVTDIDKLGRISLSRKDALPDEEEEERN

Sequences:

>Translated_700_residues
MEERTFEMELAGRKLLVQIGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTVEYEERLYSVGKIPGGFIKRE
GKPSEKAILSARLIDRPIRPLFPHGYRNDVQVIATVLSVDPDVQPEIVAMIGSSVALSISDIPFDGPTGAVAVGLVDGQF
IINPNHEQREKSLMHLVVSGTKDAIVMVEAGAKEVPEETMLDAIMYAHEYIKQIVEFIEGIVKEVGVPKSEVILHEIDKE
LEEKVRAYATEKIYNALRTAEKKERNDNLDKVEQEVLEHFKEEYPDNLADIDEVLYNIMKEQMRKMITEERIRVDGRGLD
DIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGALGDIQILDGIGDEEAKRYMHHYNFPPYSVGEVRPLRGPGRREIG
HGALAERALEPVIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGVAMGLIKEGDVVSVLTDIQ
GIEDFLGDMDFKVAGTEKGITAIQMDIKIPGIDKEILKMALEKARRGRLYILSKMLEVIKEPRKQLSVYAPRVIRMVVDP
EKIREIIGPGGKTISKIIAETGVKIDIEEDGRLYITASDLRSGERAKQMIEAITKDIAVGEIYLGKVLRITPFGAFVEIA
PGKEGLVHISKLSKKRVQKVEDVVKVGDDILVKVTDIDKLGRISLSRKDALPDEEEEERN
>Mature_700_residues
MEERTFEMELAGRKLLVQIGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTVEYEERLYSVGKIPGGFIKRE
GKPSEKAILSARLIDRPIRPLFPHGYRNDVQVIATVLSVDPDVQPEIVAMIGSSVALSISDIPFDGPTGAVAVGLVDGQF
IINPNHEQREKSLMHLVVSGTKDAIVMVEAGAKEVPEETMLDAIMYAHEYIKQIVEFIEGIVKEVGVPKSEVILHEIDKE
LEEKVRAYATEKIYNALRTAEKKERNDNLDKVEQEVLEHFKEEYPDNLADIDEVLYNIMKEQMRKMITEERIRVDGRGLD
DIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGALGDIQILDGIGDEEAKRYMHHYNFPPYSVGEVRPLRGPGRREIG
HGALAERALEPVIPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGVAMGLIKEGDVVSVLTDIQ
GIEDFLGDMDFKVAGTEKGITAIQMDIKIPGIDKEILKMALEKARRGRLYILSKMLEVIKEPRKQLSVYAPRVIRMVVDP
EKIREIIGPGGKTISKIIAETGVKIDIEEDGRLYITASDLRSGERAKQMIEAITKDIAVGEIYLGKVLRITPFGAFVEIA
PGKEGLVHISKLSKKRVQKVEDVVKVGDDILVKVTDIDKLGRISLSRKDALPDEEEEERN

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=40.0280898876404, Blast_Score=479, Evalue=1e-135,
Organism=Escherichia coli, GI145693187, Length=675, Percent_Identity=49.7777777777778, Blast_Score=663, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=717, Percent_Identity=34.1701534170153, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI281362905, Length=703, Percent_Identity=40.1137980085349, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24651641, Length=703, Percent_Identity=40.1137980085349, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24651643, Length=703, Percent_Identity=40.1137980085349, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI161079377, Length=648, Percent_Identity=40.1234567901235, Blast_Score=436, Evalue=1e-122,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77499; Mature: 77499

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERTFEMELAGRKLLVQIGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTV
CCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCCCCCEEEEEE
EYEERLYSVGKIPGGFIKREGKPSEKAILSARLIDRPIRPLFPHGYRNDVQVIATVLSVD
EHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDVQPEIVAMIGSSVALSISDIPFDGPTGAVAVGLVDGQFIINPNHEQREKSLMHLVVSG
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHCCC
TKDAIVMVEAGAKEVPEETMLDAIMYAHEYIKQIVEFIEGIVKEVGVPKSEVILHEIDKE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LEEKVRAYATEKIYNALRTAEKKERNDNLDKVEQEVLEHFKEEYPDNLADIDEVLYNIMK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQMRKMITEERIRVDGRGLDDIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGALGDI
HHHHHHHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCEEEEEEHHCCCCCE
QILDGIGDEEAKRYMHHYNFPPYSVGEVRPLRGPGRREIGHGALAERALEPVIPSEEEFP
EEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
YTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGVAMGLIKEGDVVSVLTDIQ
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
GIEDFLGDMDFKVAGTEKGITAIQMDIKIPGIDKEILKMALEKARRGRLYILSKMLEVIK
HHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
EPRKQLSVYAPRVIRMVVDPEKIREIIGPGGKTISKIIAETGVKIDIEEDGRLYITASDL
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEEHHH
RSGERAKQMIEAITKDIAVGEIYLGKVLRITPFGAFVEIAPGKEGLVHISKLSKKRVQKV
CCCHHHHHHHHHHHHHHHHCHHHHCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHH
EDVVKVGDDILVKVTDIDKLGRISLSRKDALPDEEEEERN
HHHHHCCCCEEEEEECCCCCCCCCCCHHCCCCCCHHHCCC
>Mature Secondary Structure
MEERTFEMELAGRKLLVQIGKVAQQANGAAWVKYGDTVVLVTACASKEPREGIDFFPLTV
CCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCCCCCCEEEEEE
EYEERLYSVGKIPGGFIKREGKPSEKAILSARLIDRPIRPLFPHGYRNDVQVIATVLSVD
EHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
PDVQPEIVAMIGSSVALSISDIPFDGPTGAVAVGLVDGQFIINPNHEQREKSLMHLVVSG
CCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHCCC
TKDAIVMVEAGAKEVPEETMLDAIMYAHEYIKQIVEFIEGIVKEVGVPKSEVILHEIDKE
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LEEKVRAYATEKIYNALRTAEKKERNDNLDKVEQEVLEHFKEEYPDNLADIDEVLYNIMK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EQMRKMITEERIRVDGRGLDDIRPIWCEVGVLPRTHGSAIFTRGQTQVLTVATLGALGDI
HHHHHHHHHHHHEECCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCEEEEEEHHCCCCCE
QILDGIGDEEAKRYMHHYNFPPYSVGEVRPLRGPGRREIGHGALAERALEPVIPSEEEFP
EEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
YTIRLVSEVLSSNGSTSQASVCGSTLALMDAGVPIKAPVAGVAMGLIKEGDVVSVLTDIQ
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
GIEDFLGDMDFKVAGTEKGITAIQMDIKIPGIDKEILKMALEKARRGRLYILSKMLEVIK
HHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
EPRKQLSVYAPRVIRMVVDPEKIREIIGPGGKTISKIIAETGVKIDIEEDGRLYITASDL
HHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEEHHH
RSGERAKQMIEAITKDIAVGEIYLGKVLRITPFGAFVEIAPGKEGLVHISKLSKKRVQKV
CCCHHHHHHHHHHHHHHHHCHHHHCCEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHH
EDVVKVGDDILVKVTDIDKLGRISLSRKDALPDEEEEERN
HHHHHCCCCEEEEEECCCCCCCCCCCHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]