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The map label for this gene is rimO [H]

Identifier: 167040267

GI number: 167040267

Start: 1647268

End: 1648578

Strand: Reverse

Name: rimO [H]

Synonym: Teth514_1630

Alternate gene names: 167040267

Gene position: 1648578-1647268 (Counterclockwise)

Preceding gene: 167040268

Following gene: 167040266

Centisome position: 67.09

GC content: 31.73

Gene sequence:

>1311_bases
ATGAAAAATGTAGGAATTATATCTCTTGGATGTCACAAAAACACAATTGATTCAGAAAAAATGTTAGCAATTTTAAAAGA
AAAAGGTTATAACATAGTAAATGATGAAAATAAAGCTGACATACTCATTATAAATACTTGTGGCTTTATAGAAGATGCAA
AAAGAGAGTCGATAGAATGTATCATTGAGATGGGAAAACTCAAAAAACACAGGCTAAAATACCTTATTGCAACTGGCTGT
TTGTCTGAAAGATATAATAAAGAATTATTAAAGGAACTTCCTGAATTAGATGCAGTAATTGGCACTGGAGATTTCCACAA
AATTGCAGAAGTAATAGAAAAAATAGAAAAAGGAAAAACTGTTTTAGAATATGGACACGCTAATTTACTAAATGATGAAG
GAATACAGCGCATTTTAACTACGCCAAATTATTACGCCTATTTAAAAATTGCAGAAGGTTGCAGCAATGCTTGTTCTTTT
TGCATAATTCCAAGGCTTAGAGGAAGATACAGAAGTCGCAAAATGGAGGATATACTCAGAGAAGCCGAGGAACTCGTCAA
AAAAGGTGTAAAAGAACTTATACTCATTGCTCAAGACACTACAAAATATGGAGTAGACGTTTATAAAAAATTTATGCTGC
CCCAACTTTTAAAAGAAATTTCTAAGATTGATGGTTTAAAGTGGATTAGGTTACTTTATGCATATCCTGATAGTGTAACA
GAAGAACTTGTTGAAGAGATAAAAAACAATGAAAAAATAGTAAAATATATTGATATTCCGCTACAACATTCTCATGATGA
AGTCCTAAAAAGAATGAATAGAAATACTAATAAACAAAAAATAGAAGAGGTTATAAGTAGATTGCGTAGCATTCCATACA
TGGTAATACGCACTACTTTTATGGTAGGCTTCCCTGGAGAGACAGAAGAAGAATTTGAGGATTTAAAACAATTTGTAAAA
GAAAAAAGATTTGAGAGAGTAGGAGTTTTCACATATTCTCGAGAAGAGGGAACTAAATCGTATTATATGAAGCCCCAAAT
TAAAAAGAGTGTCAAAATTAAAAGACAACAAGAGTTGATGGAGATACAAAAAGAAATTTCTTATCAGCATAATCTTTCAA
AAGTGGGAAAGCAGTTAGAAGTACTAATTGAAGGTTTTGAAGATGGAATATATTATGGCAGAAGTTATATGGATGCCCCT
GAGATTGATGGTGTTGTTTATGTAAAAAGCGACAAAAAACTCAAAGCTGGAGATTTTGTGGTAGCAACTATAACAGATGC
TTATGAGTACGATTTGGTGGGGGAATATTAA

Upstream 100 bases:

>100_bases
TATAAGCGGTTATGATGGTTCTAAGCCAAGGCAAATTCTTGTATCAAAAGAAGAAATAAAAAAAATATTAGAAGAATCTT
AAAATAGAAAGGAAATGCAG

Downstream 100 bases:

>100_bases
TGAATTTAGCAAACAAACTTACAATATTAAGACTTTTTTTAATACCTATTTTTATGTTTTTTATGCTTGTCAGAGGTCCC
TACAACGATTTTATAGCTGC

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKNVGIISLGCHKNTIDSEKMLAILKEKGYNIVNDENKADILIINTCGFIEDAKRESIECIIEMGKLKKHRLKYLIATGC
LSERYNKELLKELPELDAVIGTGDFHKIAEVIEKIEKGKTVLEYGHANLLNDEGIQRILTTPNYYAYLKIAEGCSNACSF
CIIPRLRGRYRSRKMEDILREAEELVKKGVKELILIAQDTTKYGVDVYKKFMLPQLLKEISKIDGLKWIRLLYAYPDSVT
EELVEEIKNNEKIVKYIDIPLQHSHDEVLKRMNRNTNKQKIEEVISRLRSIPYMVIRTTFMVGFPGETEEEFEDLKQFVK
EKRFERVGVFTYSREEGTKSYYMKPQIKKSVKIKRQQELMEIQKEISYQHNLSKVGKQLEVLIEGFEDGIYYGRSYMDAP
EIDGVVYVKSDKKLKAGDFVVATITDAYEYDLVGEY

Sequences:

>Translated_436_residues
MKNVGIISLGCHKNTIDSEKMLAILKEKGYNIVNDENKADILIINTCGFIEDAKRESIECIIEMGKLKKHRLKYLIATGC
LSERYNKELLKELPELDAVIGTGDFHKIAEVIEKIEKGKTVLEYGHANLLNDEGIQRILTTPNYYAYLKIAEGCSNACSF
CIIPRLRGRYRSRKMEDILREAEELVKKGVKELILIAQDTTKYGVDVYKKFMLPQLLKEISKIDGLKWIRLLYAYPDSVT
EELVEEIKNNEKIVKYIDIPLQHSHDEVLKRMNRNTNKQKIEEVISRLRSIPYMVIRTTFMVGFPGETEEEFEDLKQFVK
EKRFERVGVFTYSREEGTKSYYMKPQIKKSVKIKRQQELMEIQKEISYQHNLSKVGKQLEVLIEGFEDGIYYGRSYMDAP
EIDGVVYVKSDKKLKAGDFVVATITDAYEYDLVGEY
>Mature_436_residues
MKNVGIISLGCHKNTIDSEKMLAILKEKGYNIVNDENKADILIINTCGFIEDAKRESIECIIEMGKLKKHRLKYLIATGC
LSERYNKELLKELPELDAVIGTGDFHKIAEVIEKIEKGKTVLEYGHANLLNDEGIQRILTTPNYYAYLKIAEGCSNACSF
CIIPRLRGRYRSRKMEDILREAEELVKKGVKELILIAQDTTKYGVDVYKKFMLPQLLKEISKIDGLKWIRLLYAYPDSVT
EELVEEIKNNEKIVKYIDIPLQHSHDEVLKRMNRNTNKQKIEEVISRLRSIPYMVIRTTFMVGFPGETEEEFEDLKQFVK
EKRFERVGVFTYSREEGTKSYYMKPQIKKSVKIKRQQELMEIQKEISYQHNLSKVGKQLEVLIEGFEDGIYYGRSYMDAP
EIDGVVYVKSDKKLKAGDFVVATITDAYEYDLVGEY

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=471, Percent_Identity=26.5392781316348, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI28872784, Length=471, Percent_Identity=26.5392781316348, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI93277076, Length=411, Percent_Identity=27.9805352798054, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=38.9261744966443, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=26.7415730337079, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=23.0277185501066, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71996771, Length=280, Percent_Identity=28.2142857142857, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356207, Length=473, Percent_Identity=26.4270613107822, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=27.5330396475771, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50562; Mature: 50562

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVGIISLGCHKNTIDSEKMLAILKEKGYNIVNDENKADILIINTCGFIEDAKRESIEC
CCCCCEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHH
IIEMGKLKKHRLKYLIATGCLSERYNKELLKELPELDAVIGTGDFHKIAEVIEKIEKGKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCHHHHHHHHHHHHCCCC
VLEYGHANLLNDEGIQRILTTPNYYAYLKIAEGCSNACSFCIIPRLRGRYRSRKMEDILR
EEHHCCCCCCCHHHHHHHHCCCCEEEEEEEHHCCCCHHCEEEEHHHHHHHHHHHHHHHHH
EAEELVKKGVKELILIAQDTTKYGVDVYKKFMLPQLLKEISKIDGLKWIRLLYAYPDSVT
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHH
EELVEEIKNNEKIVKYIDIPLQHSHDEVLKRMNRNTNKQKIEEVISRLRSIPYMVIRTTF
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
MVGFPGETEEEFEDLKQFVKEKRFERVGVFTYSREEGTKSYYMKPQIKKSVKIKRQQELM
EEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEECHHHHHHHHHHHHHHHH
EIQKEISYQHNLSKVGKQLEVLIEGFEDGIYYGRSYMDAPEIDGVVYVKSDKKLKAGDFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEE
VATITDAYEYDLVGEY
EEEEECCHHCCCCCCC
>Mature Secondary Structure
MKNVGIISLGCHKNTIDSEKMLAILKEKGYNIVNDENKADILIINTCGFIEDAKRESIEC
CCCCCEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHH
IIEMGKLKKHRLKYLIATGCLSERYNKELLKELPELDAVIGTGDFHKIAEVIEKIEKGKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECCCCHHHHHHHHHHHHCCCC
VLEYGHANLLNDEGIQRILTTPNYYAYLKIAEGCSNACSFCIIPRLRGRYRSRKMEDILR
EEHHCCCCCCCHHHHHHHHCCCCEEEEEEEHHCCCCHHCEEEEHHHHHHHHHHHHHHHHH
EAEELVKKGVKELILIAQDTTKYGVDVYKKFMLPQLLKEISKIDGLKWIRLLYAYPDSVT
HHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHH
EELVEEIKNNEKIVKYIDIPLQHSHDEVLKRMNRNTNKQKIEEVISRLRSIPYMVIRTTF
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
MVGFPGETEEEFEDLKQFVKEKRFERVGVFTYSREEGTKSYYMKPQIKKSVKIKRQQELM
EEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEECHHHHHHHHHHHHHHHH
EIQKEISYQHNLSKVGKQLEVLIEGFEDGIYYGRSYMDAPEIDGVVYVKSDKKLKAGDFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCEE
VATITDAYEYDLVGEY
EEEEECCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA