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The map label for this gene is purB [H]

Identifier: 167040257

GI number: 167040257

Start: 1637315

End: 1638736

Strand: Reverse

Name: purB [H]

Synonym: Teth514_1620

Alternate gene names: 167040257

Gene position: 1638736-1637315 (Counterclockwise)

Preceding gene: 167040258

Following gene: 167040256

Centisome position: 66.69

GC content: 33.76

Gene sequence:

>1422_bases
ATGCGAGATACTTATCAGAATCCGTTAATAACAAGATATGCTAGCAAAGAAATGGCCCAAATCTTCTCTGACGACGAAAA
ATTTAAAACTTGGCGAAGATTATGGGTAGCTTTGGCAAAAAGCGAAAAAGAATTAGGGCTCAATATTACAGATGAGCAGA
TTAAAGAAATGGAAGAAAATATAGACAACATAAACTATGAAGATGCGGAAAAATTCGAAAGAGAATTTCGACATGATGTT
ATGGCTCACATACATGCCTATGGTCTTTTGTGCCCCAAAGCAAAACCTATAATCCATTTAGGAGCTACCTCTGCTTTTGT
AGATGACAATACTGATATAATTCTTATGGATAGAGCTCTTGAACTAATTGAAAAGAAACTTCTCAAAGTAATAAAAATTT
TATCCGAATTTGCACTAACTTATAAGGATTTACCCACTTTAGGTTATACTCACTTTCAACCAGCTCAACTTACTACAGTT
GGTAAAAGAGCATGCCTTTGGATACAAGATTTAATTCTCGATTTACAAGATTTAAGATATAGAAGAGAAAACATAATGTT
AAGAGGAGTAAAAGGCACCACTGGTACACAAGCAAGTTTTATGGAACTTTTTGGTGGTGATGAAGAAAAAGTAAAAGAGC
TAGACAGATTAGTAATAGAAAAAATGGGATATAAAAAGTCCTTCCCTGTGACAGGACAAACTTATACGAGAAAATATGAC
TTTATGCTCTTGTCTGTTTTAAGCGGTATTGCTCAAAGTGCTCACAAATTCAGCAACGACCTAAGGCTTTTGCAACATTT
AAGAGAAATTGAAGAGCCCTTTGAAGAAAAACAAATAGGCTCCTCTGCAATGGCTTATAAAAGAAATCCTATGAGAAGCG
AGAGAATGGCAGCTTTAAGTAGGTATGTTATTGTCACACTTTTAAATGCATCTATTACTTCTTCTGCTCAATGGTTTGAA
AGAACTTTAGATGATTCAGCTAACAGGCGAATTGTAATACCCGAAATGTTCCTTGCCACAGATGCTATATTAAATCTTTA
TATAAACATTGCGTCTGGTTTAAAAGTAAACAAAAAAGTAATTGAAAAAAATGTGATGAAGGAATTGCCTTTTATGGCTA
CAGAAAAGATTTTAATGGAAGCAGTACTTAAAGGTGGGGACAGACAAGAGTTACATGAAGCTATAAGAGTATACTCCTTA
AAAGCGGCAGAAGAAGTTAAAAATGGAGAAGAAAATAGGTTGCTTGATTATATACAAAATGACCAAAGATTTAAACTTTC
CCAAGAAGAAATTAAAGAATTAGTTGACCCTATAAAATTTACCGGAAGAGCTGTAAGCCAGACTGAAGAATACATTAAGG
AAATTGTAAACCCTATTTTAGCAGATTTTAAAGAAGATGTAGAAGCAAAAGTAGAAGTGTAA

Upstream 100 bases:

>100_bases
AATTACATTAGGATTTCTTATGCTACTTCCATTGAAAATATTGAAAAAGGCTTAAATAGAATCGAAGAATTTTTAAATAA
AGCATAATGAGGAGGTAAAT

Downstream 100 bases:

>100_bases
AATTTGTTCACTTTCCACATCCCAATATCATAGAATAACAATAAAATAAAAATGGTATTGGGATGTGGTGGTAGATGGGA
ATATGGCCAGTTTTTTTTGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MRDTYQNPLITRYASKEMAQIFSDDEKFKTWRRLWVALAKSEKELGLNITDEQIKEMEENIDNINYEDAEKFEREFRHDV
MAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRALELIEKKLLKVIKILSEFALTYKDLPTLGYTHFQPAQLTTV
GKRACLWIQDLILDLQDLRYRRENIMLRGVKGTTGTQASFMELFGGDEEKVKELDRLVIEKMGYKKSFPVTGQTYTRKYD
FMLLSVLSGIAQSAHKFSNDLRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALSRYVIVTLLNASITSSAQWFE
RTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNKKVIEKNVMKELPFMATEKILMEAVLKGGDRQELHEAIRVYSL
KAAEEVKNGEENRLLDYIQNDQRFKLSQEEIKELVDPIKFTGRAVSQTEEYIKEIVNPILADFKEDVEAKVEV

Sequences:

>Translated_473_residues
MRDTYQNPLITRYASKEMAQIFSDDEKFKTWRRLWVALAKSEKELGLNITDEQIKEMEENIDNINYEDAEKFEREFRHDV
MAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRALELIEKKLLKVIKILSEFALTYKDLPTLGYTHFQPAQLTTV
GKRACLWIQDLILDLQDLRYRRENIMLRGVKGTTGTQASFMELFGGDEEKVKELDRLVIEKMGYKKSFPVTGQTYTRKYD
FMLLSVLSGIAQSAHKFSNDLRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALSRYVIVTLLNASITSSAQWFE
RTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNKKVIEKNVMKELPFMATEKILMEAVLKGGDRQELHEAIRVYSL
KAAEEVKNGEENRLLDYIQNDQRFKLSQEEIKELVDPIKFTGRAVSQTEEYIKEIVNPILADFKEDVEAKVEV
>Mature_473_residues
MRDTYQNPLITRYASKEMAQIFSDDEKFKTWRRLWVALAKSEKELGLNITDEQIKEMEENIDNINYEDAEKFEREFRHDV
MAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRALELIEKKLLKVIKILSEFALTYKDLPTLGYTHFQPAQLTTV
GKRACLWIQDLILDLQDLRYRRENIMLRGVKGTTGTQASFMELFGGDEEKVKELDRLVIEKMGYKKSFPVTGQTYTRKYD
FMLLSVLSGIAQSAHKFSNDLRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALSRYVIVTLLNASITSSAQWFE
RTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNKKVIEKNVMKELPFMATEKILMEAVLKGGDRQELHEAIRVYSL
KAAEEVKNGEENRLLDYIQNDQRFKLSQEEIKELVDPIKFTGRAVSQTEEYIKEIVNPILADFKEDVEAKVEV

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=472, Percent_Identity=55.0847457627119, Blast_Score=552, Evalue=1e-157,
Organism=Homo sapiens, GI183227688, Length=387, Percent_Identity=58.9147286821705, Blast_Score=496, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17508577, Length=476, Percent_Identity=43.2773109243698, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32564234, Length=345, Percent_Identity=47.2463768115942, Blast_Score=318, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6323391, Length=467, Percent_Identity=53.5331905781585, Blast_Score=499, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24647570, Length=462, Percent_Identity=55.8441558441558, Blast_Score=518, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54684; Mature: 54684

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDTYQNPLITRYASKEMAQIFSDDEKFKTWRRLWVALAKSEKELGLNITDEQIKEMEEN
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
IDNINYEDAEKFEREFRHDVMAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRAL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCCEEEHHHHH
ELIEKKLLKVIKILSEFALTYKDLPTLGYTHFQPAQLTTVGKRACLWIQDLILDLQDLRY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRENIMLRGVKGTTGTQASFMELFGGDEEKVKELDRLVIEKMGYKKSFPVTGQTYTRKYD
HHHHHEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
FMLLSVLSGIAQSAHKFSNDLRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCHHHHHHHHH
RYVIVTLLNASITSSAQWFERTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNKKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHH
IEKNVMKELPFMATEKILMEAVLKGGDRQELHEAIRVYSLKAAEEVKNGEENRLLDYIQN
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
DQRFKLSQEEIKELVDPIKFTGRAVSQTEEYIKEIVNPILADFKEDVEAKVEV
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRDTYQNPLITRYASKEMAQIFSDDEKFKTWRRLWVALAKSEKELGLNITDEQIKEMEEN
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
IDNINYEDAEKFEREFRHDVMAHIHAYGLLCPKAKPIIHLGATSAFVDDNTDIILMDRAL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCCEEEHHHHH
ELIEKKLLKVIKILSEFALTYKDLPTLGYTHFQPAQLTTVGKRACLWIQDLILDLQDLRY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RRENIMLRGVKGTTGTQASFMELFGGDEEKVKELDRLVIEKMGYKKSFPVTGQTYTRKYD
HHHHHEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
FMLLSVLSGIAQSAHKFSNDLRLLQHLREIEEPFEEKQIGSSAMAYKRNPMRSERMAALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCHHHHHHHHH
RYVIVTLLNASITSSAQWFERTLDDSANRRIVIPEMFLATDAILNLYINIASGLKVNKKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCHHHHHH
IEKNVMKELPFMATEKILMEAVLKGGDRQELHEAIRVYSLKAAEEVKNGEENRLLDYIQN
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
DQRFKLSQEEIKELVDPIKFTGRAVSQTEEYIKEIVNPILADFKEDVEAKVEV
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]