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The map label for this gene is kipI [H]

Identifier: 167040233

GI number: 167040233

Start: 1614198

End: 1614929

Strand: Direct

Name: kipI [H]

Synonym: Teth514_1595

Alternate gene names: 167040233

Gene position: 1614198-1614929 (Clockwise)

Preceding gene: 167040232

Following gene: 167040234

Centisome position: 65.69

GC content: 32.65

Gene sequence:

>732_bases
ATGTATGAAAAACCTAAAATTCTCTTATGCGGGGACAAGGCTGTTATAGTTGAATTTGGAAATGAAATAAGTGAGGAATG
CAATAAAAAAGTAGTAAATCTTTATAGGACGTTAGAAAAAGAGAAGAGTGCAGGAATTATTTCTATGATTCCAACTTATA
GATCTTTGCTTATTAAATATGATCCTTTAAAAATTGCTTACGATGAACTATTAAAACTAATTGAATCAAGCAATAAAGAT
AAGAATGAAAGTACAGAAGAATTTAGGTCTAAAATATATGAAATTCCGGTAGCTTATGGAGGAGAATTTGGCCCAGATTT
AGATTTTGTAGCTAAATACCATAATATGACTGCTGAAGAGATAATAAGTATTCATACACAACCTTTTTATAGAATTTATA
TGGTTGGTTTCACAATGGGATTTGCGTATTTAGGAGGTATGTCTGAAAAAATTGCTACTCCACGATTAGAAAATCCGAGA
ACTGAGATAAAGGCAGGGTCAGTAGGCATTGCAGGTAACCAAACAGGTATCTATCCGCTTTCTAGTCCTGGTGGCTGGAG
GATAATAGGGAGGACTCCTGTAAGATTATATGATCCAGAAAGAGAGAGGCCTATTCTTTTTGAAGCAGGTAATTACATAA
AGTTTGTGCCGATAACAGGAGAGGAATTTTACAAGATTGAAAAAGAAATAGAAATGAAAAGATATCAAATTAAAATATAC
GATTATGAGTAA

Upstream 100 bases:

>100_bases
GGCTGTAGAATTTGCTAAAAGAATAAAAAAGACTTTAGAAGAAAATGGAGTTGAAATAATACCCATCGGTGAGTTTTTAT
AATACAGGGAGTTGATTATA

Downstream 100 bases:

>100_bases
CGAAACTTTCTCTAAGAACAGTAAAAAAACCAAAAATAGTAGAAACTTTGCAAGGTAGGTGAAAAAATGGAGTGCTTTAG
AGTATTAAGTCAGGGTCTTT

Product: allophanate hydrolase subunit 1

Products: CO2; NH3

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MYEKPKILLCGDKAVIVEFGNEISEECNKKVVNLYRTLEKEKSAGIISMIPTYRSLLIKYDPLKIAYDELLKLIESSNKD
KNESTEEFRSKIYEIPVAYGGEFGPDLDFVAKYHNMTAEEIISIHTQPFYRIYMVGFTMGFAYLGGMSEKIATPRLENPR
TEIKAGSVGIAGNQTGIYPLSSPGGWRIIGRTPVRLYDPERERPILFEAGNYIKFVPITGEEFYKIEKEIEMKRYQIKIY
DYE

Sequences:

>Translated_243_residues
MYEKPKILLCGDKAVIVEFGNEISEECNKKVVNLYRTLEKEKSAGIISMIPTYRSLLIKYDPLKIAYDELLKLIESSNKD
KNESTEEFRSKIYEIPVAYGGEFGPDLDFVAKYHNMTAEEIISIHTQPFYRIYMVGFTMGFAYLGGMSEKIATPRLENPR
TEIKAGSVGIAGNQTGIYPLSSPGGWRIIGRTPVRLYDPERERPILFEAGNYIKFVPITGEEFYKIEKEIEMKRYQIKIY
DYE
>Mature_243_residues
MYEKPKILLCGDKAVIVEFGNEISEECNKKVVNLYRTLEKEKSAGIISMIPTYRSLLIKYDPLKIAYDELLKLIESSNKD
KNESTEEFRSKIYEIPVAYGGEFGPDLDFVAKYHNMTAEEIISIHTQPFYRIYMVGFTMGFAYLGGMSEKIATPRLENPR
TEIKAGSVGIAGNQTGIYPLSSPGGWRIIGRTPVRLYDPERERPILFEAGNYIKFVPITGEEFYKIEKEIEMKRYQIKIY
DYE

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=216, Percent_Identity=35.6481481481481, Blast_Score=153, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319685, Length=270, Percent_Identity=25.9259259259259, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: 3.5.1.54

Molecular weight: Translated: 27966; Mature: 27966

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEKPKILLCGDKAVIVEFGNEISEECNKKVVNLYRTLEKEKSAGIISMIPTYRSLLIKY
CCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHEEC
DPLKIAYDELLKLIESSNKDKNESTEEFRSKIYEIPVAYGGEFGPDLDFVAKYHNMTAEE
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHH
IISIHTQPFYRIYMVGFTMGFAYLGGMSEKIATPRLENPRTEIKAGSVGIAGNQTGIYPL
HHHHCCCCCEEEHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHEECCCEEECCCCCCEEEC
SSPGGWRIIGRTPVRLYDPERERPILFEAGNYIKFVPITGEEFYKIEKEIEMKRYQIKIY
CCCCCEEEEECCCEEEECCCCCCCEEEECCCEEEEEEECCHHHHHHHHHHCEEEEEEEEE
DYE
ECC
>Mature Secondary Structure
MYEKPKILLCGDKAVIVEFGNEISEECNKKVVNLYRTLEKEKSAGIISMIPTYRSLLIKY
CCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHEEC
DPLKIAYDELLKLIESSNKDKNESTEEFRSKIYEIPVAYGGEFGPDLDFVAKYHNMTAEE
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHH
IISIHTQPFYRIYMVGFTMGFAYLGGMSEKIATPRLENPRTEIKAGSVGIAGNQTGIYPL
HHHHCCCCCEEEHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHEECCCEEECCCCCCEEEC
SSPGGWRIIGRTPVRLYDPERERPILFEAGNYIKFVPITGEEFYKIEKEIEMKRYQIKIY
CCCCCEEEEECCCEEEECCCCCCCEEEECCCEEEEEEECCHHHHHHHHHHCEEEEEEEEE
DYE
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: urea-1-carboxylate; H2O

Specific reaction: urea-1-carboxylate + H2O = 2 CO2 + 2 NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]