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The map label for this gene is cca [H]

Identifier: 167040202

GI number: 167040202

Start: 1584194

End: 1585360

Strand: Direct

Name: cca [H]

Synonym: Teth514_1564

Alternate gene names: 167040202

Gene position: 1584194-1585360 (Clockwise)

Preceding gene: 167040201

Following gene: 167040203

Centisome position: 64.47

GC content: 29.05

Gene sequence:

>1167_bases
TTGCGTATAGAGGTTAACGGAATAGATTTAATTAAACTGCTGAAGGAAATTTCTTCAAAAACAAAAATTAAATCTTATAT
TGTAGGTGGAGTGGTAAGAGACTTTTTATTAGGGGTTAAAAACCTTGATATTGATGTAGTAGTGGAAGGAGACGGTATTA
AGTTTGCCTATACATTGCTTTCTTATTTAAAAGGTGATATTGTAGTGTATGAAGCTTTTAGGACTGCTACCTTAAGTTAT
GACAGTATTTCTATTGATGTTATATCTGCTCGTAAAGAATATTATGAACGTCCTGCTGCACTGCCAACAATCGAATTTTC
AAATATATACGATGACATGGCAAGAAGAGATTTTACTATAAACACTTTGGCTTATGATGTAATAGAAGAGAAGATTTTGG
ATTATTTTAATGGATTAGAGGACTTAAAAAAGGGTTTCATTAGAGTCCTTCATCCTAAAAGTTTTATTGATGACCCCACC
AGAATATTTAGAGCTATAAGATATGCTACTAGATATTCTTTTGAGATTGAAGAAAAGACACACAATTTGATGAAGGAATC
AATAGAAAATATTAGACTTTTGTCTGCTGATAGAATAAGGAATGAGATCTTTTTAATTTTGAAAGAAAGTAAAGTCAAAG
AAATGATTGAAAAACTTATTTCTTACGGTATTGATAAAGTTATTTTTGATGGAATTACACTTAATACTCAAAATCTCGAT
ACAGTAGATGTAAATTTAGAAGTTGAATTATACAGATTTTTAGTGCTTTTTTACTACCTAAAAGAGAGTGATATTAATAA
AATTGAAAAGAGGTTGCGAGTAAATAAAATTTATCTCAAAGCTTTAAAAGACTTGGTTTTTTTAAGAGAGCAACTCAATT
GCCAAAATAAAAATATCAGAAAAATAAGAGAAACTATAGAAGAAACAAAAGAGGAAGTTTTAAGAGCTATTGAAGTAATG
GAAGGAGAAAAAGCCGAAAAAATTATAAAAGGTAAATTGACTGTAAAGGGAAAAGACATAAAAAATTTAGGACTTCCTCC
CAGTCCTTTATATGGGGAATTAATGGATAATGTGTTTGAGGCGAAAATTAATGGAATTATTGCTACAAGAGATGAAGAAA
TTGCATTTTTGAAAAAACTGATAGAAAAATTAAAGAAAGGAGAATAA

Upstream 100 bases:

>100_bases
GTATAGGTTAATGAATTTTAACTTATACTTATTTTTTTATCTTTTCCTATGGTATAATATAAAAAGCAAGGAATTATTTT
GTTGGTAAAAGGGTGATTTT

Downstream 100 bases:

>100_bases
ATTTGATAGATTTTATAGATTTTTTATATAGAATACCTGCACTTCTTATTGCGATGAGTTTCCACGAATTTAGTCATGGT
TATGTGGCAGATAAATTGGG

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MRIEVNGIDLIKLLKEISSKTKIKSYIVGGVVRDFLLGVKNLDIDVVVEGDGIKFAYTLLSYLKGDIVVYEAFRTATLSY
DSISIDVISARKEYYERPAALPTIEFSNIYDDMARRDFTINTLAYDVIEEKILDYFNGLEDLKKGFIRVLHPKSFIDDPT
RIFRAIRYATRYSFEIEEKTHNLMKESIENIRLLSADRIRNEIFLILKESKVKEMIEKLISYGIDKVIFDGITLNTQNLD
TVDVNLEVELYRFLVLFYYLKESDINKIEKRLRVNKIYLKALKDLVFLREQLNCQNKNIRKIRETIEETKEEVLRAIEVM
EGEKAEKIIKGKLTVKGKDIKNLGLPPSPLYGELMDNVFEAKINGIIATRDEEIAFLKKLIEKLKKGE

Sequences:

>Translated_388_residues
MRIEVNGIDLIKLLKEISSKTKIKSYIVGGVVRDFLLGVKNLDIDVVVEGDGIKFAYTLLSYLKGDIVVYEAFRTATLSY
DSISIDVISARKEYYERPAALPTIEFSNIYDDMARRDFTINTLAYDVIEEKILDYFNGLEDLKKGFIRVLHPKSFIDDPT
RIFRAIRYATRYSFEIEEKTHNLMKESIENIRLLSADRIRNEIFLILKESKVKEMIEKLISYGIDKVIFDGITLNTQNLD
TVDVNLEVELYRFLVLFYYLKESDINKIEKRLRVNKIYLKALKDLVFLREQLNCQNKNIRKIRETIEETKEEVLRAIEVM
EGEKAEKIIKGKLTVKGKDIKNLGLPPSPLYGELMDNVFEAKINGIIATRDEEIAFLKKLIEKLKKGE
>Mature_388_residues
MRIEVNGIDLIKLLKEISSKTKIKSYIVGGVVRDFLLGVKNLDIDVVVEGDGIKFAYTLLSYLKGDIVVYEAFRTATLSY
DSISIDVISARKEYYERPAALPTIEFSNIYDDMARRDFTINTLAYDVIEEKILDYFNGLEDLKKGFIRVLHPKSFIDDPT
RIFRAIRYATRYSFEIEEKTHNLMKESIENIRLLSADRIRNEIFLILKESKVKEMIEKLISYGIDKVIFDGITLNTQNLD
TVDVNLEVELYRFLVLFYYLKESDINKIEKRLRVNKIYLKALKDLVFLREQLNCQNKNIRKIRETIEETKEEVLRAIEVM
EGEKAEKIIKGKLTVKGKDIKNLGLPPSPLYGELMDNVFEAKINGIIATRDEEIAFLKKLIEKLKKGE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=409, Percent_Identity=25.1833740831296, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI87081691, Length=278, Percent_Identity=27.3381294964029, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=31.140350877193, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=172, Percent_Identity=33.1395348837209, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45068; Mature: 45068

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEVNGIDLIKLLKEISSKTKIKSYIVGGVVRDFLLGVKNLDIDVVVEGDGIKFAYTLL
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
SYLKGDIVVYEAFRTATLSYDSISIDVISARKEYYERPAALPTIEFSNIYDDMARRDFTI
HHHCCCEEEEEEHHHHEEEECCEEEEHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCH
NTLAYDVIEEKILDYFNGLEDLKKGFIRVLHPKSFIDDPTRIFRAIRYATRYSFEIEEKT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCEEEHHHH
HNLMKESIENIRLLSADRIRNEIFLILKESKVKEMIEKLISYGIDKVIFDGITLNTQNLD
HHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHCCEEECCCCCC
TVDVNLEVELYRFLVLFYYLKESDINKIEKRLRVNKIYLKALKDLVFLREQLNCQNKNIR
EEEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KIRETIEETKEEVLRAIEVMEGEKAEKIIKGKLTVKGKDIKNLGLPPSPLYGELMDNVFE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
AKINGIIATRDEEIAFLKKLIEKLKKGE
HHHCCEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIEVNGIDLIKLLKEISSKTKIKSYIVGGVVRDFLLGVKNLDIDVVVEGDGIKFAYTLL
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
SYLKGDIVVYEAFRTATLSYDSISIDVISARKEYYERPAALPTIEFSNIYDDMARRDFTI
HHHCCCEEEEEEHHHHEEEECCEEEEHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCH
NTLAYDVIEEKILDYFNGLEDLKKGFIRVLHPKSFIDDPTRIFRAIRYATRYSFEIEEKT
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCEEEHHHH
HNLMKESIENIRLLSADRIRNEIFLILKESKVKEMIEKLISYGIDKVIFDGITLNTQNLD
HHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHCCEEECCCCCC
TVDVNLEVELYRFLVLFYYLKESDINKIEKRLRVNKIYLKALKDLVFLREQLNCQNKNIR
EEEEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KIRETIEETKEEVLRAIEVMEGEKAEKIIKGKLTVKGKDIKNLGLPPSPLYGELMDNVFE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
AKINGIIATRDEEIAFLKKLIEKLKKGE
HHHCCEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA