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The map label for this gene is rpoD [H]

Identifier: 167039957

GI number: 167039957

Start: 1352111

End: 1353184

Strand: Direct

Name: rpoD [H]

Synonym: Teth514_1314

Alternate gene names: 167039957

Gene position: 1352111-1353184 (Clockwise)

Preceding gene: 167039956

Following gene: 167039959

Centisome position: 55.03

GC content: 37.8

Gene sequence:

>1074_bases
ATGAACAACGAAGAATTGTCTAAAGAAGCCATTACAGAACTTATCAATAAAGGAAAAAAAACTGGGATGCTCACCTACAA
TGAGATAATGGATTCTTTAGAAGATATTGATTTAAATCCTGACCAAATTGAGAAAGTATATGATGCTTTTGAAGACATGG
GAATTGAAATTGTTGGGGAAGAACCTCGTCAGGAAGAAATAACTGAAGAAGATTTAGATTTAGACCTTTCTCTTCCTGAA
GGAATCAGTATAGATGACCCTGTTAGAATGTATTTGAAGGAGATAGGCAAGATTCCCCTTCTCACTCCTGAAGAAGAGAT
TGAATTGGCAAAAAGAATCGAACAAGGAGACGAAGAAGCTAAAAAAAGACTTATAGAAGCTAATTTGAGATTGGTTGTAA
GCATAGCCAAAAGATACGTAGGAAGAGGAATGCTTTTCTTAGATTTGATACAAGAAGGAAATTTGGGGTTACTCAAAGCA
GTTGAAAAATTTGACTATAGAAAAGGCTATAAATTTAGTACTTACGCTACATGGTGGATAAGACAGGCTATTACAAGAGC
TATTGCAGATCAAGCAAGAACCATAAGAATCCCTGTACACATGGTAGAAACTATCAATAAATTGATAAGAGTCCAAAGGC
AATTGCTGCAGGAATTAGGTCGTGAGCCCACTCCTGAAGAGTTGGCAAAAGAGATGGGGATGCCAGAAGAAAAAGTAAGA
GAGATAATGAAAATAGCACAAGAACCTGTATCTTTAGAAACTCCTATTGGGGAAGAGGAAGACAGCCACTTAGGCGATTT
TATACCCGATGAAGATGCACCAGCTCCAGCAGAAGCTGCTGCTTTTACAATGCTAAAAGAACAGTTGATGGATGTTCTTG
ATACCCTAACGCCAAGGGAAGAGAAAGTACTAAGACTTAGGTTTGGCCTAGATGACGGTCGAGCAAGAACTTTAGAGGAA
GTAGGAAAAGAGTTTAATGTTACAAGAGAACGCATAAGGCAAATTGAAGCAAAGGCTTTGAGAAAATTGAGGCATCCCAG
CAGGAGCAAAAAATTAAAAGATTTCCTTGATTGA

Upstream 100 bases:

>100_bases
AAAGAAAGGCAACTTTTAATAGAATTACAAAATTGTGAAAAGGAGATGCTAAAGATAAAAAATGGCTAAAAAGAAAGAAA
CCCATAGGGAGGGGAAACCT

Downstream 100 bases:

>100_bases
ATAAGCTTCTTGACAATAGGGCAAAAATCTGTATAATTATTATTGTATTCCTCAGTAGCTCAAGGGTAGAGCAACCGGCT
GTTAACCGGTAGGTTGTAGG

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A; Major vegetative sigma factor [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MNNEELSKEAITELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKVYDAFEDMGIEIVGEEPRQEEITEEDLDLDLSLPE
GISIDDPVRMYLKEIGKIPLLTPEEEIELAKRIEQGDEEAKKRLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLLKA
VEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTPEELAKEMGMPEEKVR
EIMKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAFTMLKEQLMDVLDTLTPREEKVLRLRFGLDDGRARTLEE
VGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD

Sequences:

>Translated_357_residues
MNNEELSKEAITELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKVYDAFEDMGIEIVGEEPRQEEITEEDLDLDLSLPE
GISIDDPVRMYLKEIGKIPLLTPEEEIELAKRIEQGDEEAKKRLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLLKA
VEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTPEELAKEMGMPEEKVR
EIMKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAFTMLKEQLMDVLDTLTPREEKVLRLRFGLDDGRARTLEE
VGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD
>Mature_357_residues
MNNEELSKEAITELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKVYDAFEDMGIEIVGEEPRQEEITEEDLDLDLSLPE
GISIDDPVRMYLKEIGKIPLLTPEEEIELAKRIEQGDEEAKKRLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLLKA
VEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTPEELAKEMGMPEEKVR
EIMKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAFTMLKEQLMDVLDTLTPREEKVLRLRFGLDDGRARTLEE
VGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=260, Percent_Identity=66.9230769230769, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1789098, Length=329, Percent_Identity=41.6413373860182, Blast_Score=253, Evalue=9e-69,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=30.5147058823529, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788231, Length=199, Percent_Identity=28.1407035175879, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41128; Mature: 41128

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEELSKEAITELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKVYDAFEDMGIEIVGE
CCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECC
EPRQEEITEEDLDLDLSLPEGISIDDPVRMYLKEIGKIPLLTPEEEIELAKRIEQGDEEA
CCCHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
KKRLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLLKAVEKFDYRKGYKFSTYATWWI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
RQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTPEELAKEMGMPEEKVR
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
EIMKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAFTMLKEQLMDVLDTLTPRE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
EKVLRLRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNNEELSKEAITELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKVYDAFEDMGIEIVGE
CCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECC
EPRQEEITEEDLDLDLSLPEGISIDDPVRMYLKEIGKIPLLTPEEEIELAKRIEQGDEEA
CCCHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHH
KKRLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLLKAVEKFDYRKGYKFSTYATWWI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
RQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTPEELAKEMGMPEEKVR
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
EIMKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAFTMLKEQLMDVLDTLTPRE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
EKVLRLRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]