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The map label for this gene is dnaG [H]

Identifier: 167039956

GI number: 167039956

Start: 1350292

End: 1352079

Strand: Direct

Name: dnaG [H]

Synonym: Teth514_1313

Alternate gene names: 167039956

Gene position: 1350292-1352079 (Clockwise)

Preceding gene: 167039955

Following gene: 167039957

Centisome position: 54.95

GC content: 31.15

Gene sequence:

>1788_bases
ATGGCTTACTCAAAAGAAATGATTGAAAAGGTCATAGAAGCAAATGACATAATAGATGTTATATCAGAGTATGTAGAACT
AAAAAAAGCGGGAAAAGAATTTAAAGGTCTATGTCCTTTTCATAGAGAAAAAACCCCATCTTTTATGGTAAGTCAAGAAA
AACAGGTTTATCACTGTTTTGGCTGTAATGCCAGTGGAAATGTTGTTACTTTTATAATGGATATTGAAAATTTGACTTTT
AAAGAAGCAATTGAATTTTTAGCAGATAGAGTAGGAATAACTCTTGAAGAAACTGTGCTTACAGAAAGGGAGTACCAAAG
AAAGAAACTTATTGACGAAATATATAAAATAAATAAATTAGCAACTATGTATTTTTACAATAAGCTTTTTTCTGAAGAAG
GTAGACAGGCTTTAACCTATATAAGAAAAAGAGGTTTGACAGAAGCAACGATAAAGAAGTTTGGAATAGGCTATTCATCA
CCTCATGGAAATGGACTTTTAAACTTTTTAAAAGAAAAAGGCTATACAGAAAGTTTTCTGGTAAAGGCTGGACTTTTGTC
TCAAAAAAATAACCGCTATTACGACAGGTTTAGAAATAGAGTAATGTTTCCTATTATAGATGTAAAGGGTAATGTTATAG
GTTTTGGAGGCCGTTCTATTGATGATAGTTTGCCTAAATACTTAAACACTCCGGAGACAGAGGTCTTTAAAAAAGGCAAA
ACGCTATTTGCAATAAATTTTGCTAAAAAAACTCAACAAGACAAATTTATAATTGTAGAAGGATACATGGACGCTATTTC
CTTACATCAAGCCGGAATAGATTGTGCTGTAGCATCTTTAGGAACCGCTTTGACAGAGGACCAAGCAAGACTCATAAAAA
GGTATAAAGGAAATGTTGTAATTGCTTATGATGCGGATGAAGCTGGTATTAGTGCCGCATTAAGAGGACTTGACATTCTT
GATGAATTGAATTTAAATATAAAAGTACTGACTATTCCTTATGGGAAAGACCCTGATGAGTTTATAAAAAAAGAAGGAGT
AAACGCATTTAATCAATTAATAGAAAATGCCGATAGTTTAATTGAGTTTAAAGCAAAGGTATTTAGAAAAAATCTCGACC
TTGATAATCCTCAAGATAGAATAATATATGTTAAAAAAATTGCAAAAGATATTGCAAAACTTTCTGATGAAGTGAAAAGG
GAAGTCTATATTTCTTCAGCTGCTAAAGTTGCTCAAATACCTGAGAATGCAGTGAGAACAGAGGTTGCGCGTTTTGTCAA
TAGGGAAATTGAAAAAAATCAAAAAAATATGTATATGGCTGGCAATATAAGGCATAATATATACAGTAGTAGTAAAATAT
CACCAGAAAAGTACTTAATTGCATTGCTGCTGTATGATAATAATCTTTACAAAAGGGTGAAAGAAACAATTACCGCCGAT
ATGCTGGAGAATGTTAAATTAAAGCCAATTTTTGGAGAAATAATGTCAAGGTTGGAGGATGGGAAAAAAACTCAAATAAA
TGATATTGTTTATTTATTACAAGAGGAAAATCTCATATCTGATTTTAATGATATTATTAAAGCTTTTTATGAGAGTGAAA
ATGTTACTGAACAAGCTGTTGATGATATTATAAACAAGATTCTCATTAACAGTTTGGCAGTTAAAAGAGAAAAGATTAAA
AGAGCTATCAATGAAGCTCATATGTTGGGGGATATAGAGAAAGAAAGGCAACTTTTAATAGAATTACAAAATTGTGAAAA
GGAGATGCTAAAGATAAAAAATGGCTAA

Upstream 100 bases:

>100_bases
TTGAATCTTTTGTCGAATATATATTGGTCGTCACTAATAAATTAATTTGGTAAAGAGGAATTTAACAATTTTTGTCGAAT
AGAATAATTAGGTGATATTG

Downstream 100 bases:

>100_bases
AAAGAAAGAAACCCATAGGGAGGGGAAACCTATGAACAACGAAGAATTGTCTAAAGAAGCCATTACAGAACTTATCAATA
AAGGAAAAAAAACTGGGATG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MAYSKEMIEKVIEANDIIDVISEYVELKKAGKEFKGLCPFHREKTPSFMVSQEKQVYHCFGCNASGNVVTFIMDIENLTF
KEAIEFLADRVGITLEETVLTEREYQRKKLIDEIYKINKLATMYFYNKLFSEEGRQALTYIRKRGLTEATIKKFGIGYSS
PHGNGLLNFLKEKGYTESFLVKAGLLSQKNNRYYDRFRNRVMFPIIDVKGNVIGFGGRSIDDSLPKYLNTPETEVFKKGK
TLFAINFAKKTQQDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKGNVVIAYDADEAGISAALRGLDIL
DELNLNIKVLTIPYGKDPDEFIKKEGVNAFNQLIENADSLIEFKAKVFRKNLDLDNPQDRIIYVKKIAKDIAKLSDEVKR
EVYISSAAKVAQIPENAVRTEVARFVNREIEKNQKNMYMAGNIRHNIYSSSKISPEKYLIALLLYDNNLYKRVKETITAD
MLENVKLKPIFGEIMSRLEDGKKTQINDIVYLLQEENLISDFNDIIKAFYESENVTEQAVDDIINKILINSLAVKREKIK
RAINEAHMLGDIEKERQLLIELQNCEKEMLKIKNG

Sequences:

>Translated_595_residues
MAYSKEMIEKVIEANDIIDVISEYVELKKAGKEFKGLCPFHREKTPSFMVSQEKQVYHCFGCNASGNVVTFIMDIENLTF
KEAIEFLADRVGITLEETVLTEREYQRKKLIDEIYKINKLATMYFYNKLFSEEGRQALTYIRKRGLTEATIKKFGIGYSS
PHGNGLLNFLKEKGYTESFLVKAGLLSQKNNRYYDRFRNRVMFPIIDVKGNVIGFGGRSIDDSLPKYLNTPETEVFKKGK
TLFAINFAKKTQQDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKGNVVIAYDADEAGISAALRGLDIL
DELNLNIKVLTIPYGKDPDEFIKKEGVNAFNQLIENADSLIEFKAKVFRKNLDLDNPQDRIIYVKKIAKDIAKLSDEVKR
EVYISSAAKVAQIPENAVRTEVARFVNREIEKNQKNMYMAGNIRHNIYSSSKISPEKYLIALLLYDNNLYKRVKETITAD
MLENVKLKPIFGEIMSRLEDGKKTQINDIVYLLQEENLISDFNDIIKAFYESENVTEQAVDDIINKILINSLAVKREKIK
RAINEAHMLGDIEKERQLLIELQNCEKEMLKIKNG
>Mature_594_residues
AYSKEMIEKVIEANDIIDVISEYVELKKAGKEFKGLCPFHREKTPSFMVSQEKQVYHCFGCNASGNVVTFIMDIENLTFK
EAIEFLADRVGITLEETVLTEREYQRKKLIDEIYKINKLATMYFYNKLFSEEGRQALTYIRKRGLTEATIKKFGIGYSSP
HGNGLLNFLKEKGYTESFLVKAGLLSQKNNRYYDRFRNRVMFPIIDVKGNVIGFGGRSIDDSLPKYLNTPETEVFKKGKT
LFAINFAKKTQQDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKGNVVIAYDADEAGISAALRGLDILD
ELNLNIKVLTIPYGKDPDEFIKKEGVNAFNQLIENADSLIEFKAKVFRKNLDLDNPQDRIIYVKKIAKDIAKLSDEVKRE
VYISSAAKVAQIPENAVRTEVARFVNREIEKNQKNMYMAGNIRHNIYSSSKISPEKYLIALLLYDNNLYKRVKETITADM
LENVKLKPIFGEIMSRLEDGKKTQINDIVYLLQEENLISDFNDIIKAFYESENVTEQAVDDIINKILINSLAVKREKIKR
AINEAHMLGDIEKERQLLIELQNCEKEMLKIKNG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=383, Percent_Identity=38.9033942558747, Blast_Score=278, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68123; Mature: 67991

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSKEMIEKVIEANDIIDVISEYVELKKAGKEFKGLCPFHREKTPSFMVSQEKQVYHCF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHCCCCCEEEEE
GCNASGNVVTFIMDIENLTFKEAIEFLADRVGITLEETVLTEREYQRKKLIDEIYKINKL
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ATMYFYNKLFSEEGRQALTYIRKRGLTEATIKKFGIGYSSPHGNGLLNFLKEKGYTESFL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHH
VKAGLLSQKNNRYYDRFRNRVMFPIIDVKGNVIGFGGRSIDDSLPKYLNTPETEVFKKGK
HHHHHHHCCCCHHHHHHHCCEEEEEEECCCCEEECCCCCCHHHHHHHCCCCHHHHHHCCC
TLFAINFAKKTQQDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKGNVV
EEEEEEEHHHCCCCCEEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IAYDADEAGISAALRGLDILDELNLNIKVLTIPYGKDPDEFIKKEGVNAFNQLIENADSL
EEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHH
IEFKAKVFRKNLDLDNPQDRIIYVKKIAKDIAKLSDEVKREVYISSAAKVAQIPENAVRT
HHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EVARFVNREIEKNQKNMYMAGNIRHNIYSSSKISPEKYLIALLLYDNNLYKRVKETITAD
HHHHHHHHHHHCCCCCEEEECCCHHCCCCCCCCCHHHHEEEEEEECCHHHHHHHHHHHHH
MLENVKLKPIFGEIMSRLEDGKKTQINDIVYLLQEENLISDFNDIIKAFYESENVTEQAV
HHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
DDIINKILINSLAVKREKIKRAINEAHMLGDIEKERQLLIELQNCEKEMLKIKNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AYSKEMIEKVIEANDIIDVISEYVELKKAGKEFKGLCPFHREKTPSFMVSQEKQVYHCF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCHHHCCCCCEEEEE
GCNASGNVVTFIMDIENLTFKEAIEFLADRVGITLEETVLTEREYQRKKLIDEIYKINKL
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ATMYFYNKLFSEEGRQALTYIRKRGLTEATIKKFGIGYSSPHGNGLLNFLKEKGYTESFL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHH
VKAGLLSQKNNRYYDRFRNRVMFPIIDVKGNVIGFGGRSIDDSLPKYLNTPETEVFKKGK
HHHHHHHCCCCHHHHHHHCCEEEEEEECCCCEEECCCCCCHHHHHHHCCCCHHHHHHCCC
TLFAINFAKKTQQDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKGNVV
EEEEEEEHHHCCCCCEEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IAYDADEAGISAALRGLDILDELNLNIKVLTIPYGKDPDEFIKKEGVNAFNQLIENADSL
EEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHH
IEFKAKVFRKNLDLDNPQDRIIYVKKIAKDIAKLSDEVKREVYISSAAKVAQIPENAVRT
HHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
EVARFVNREIEKNQKNMYMAGNIRHNIYSSSKISPEKYLIALLLYDNNLYKRVKETITAD
HHHHHHHHHHHCCCCCEEEECCCHHCCCCCCCCCHHHHEEEEEEECCHHHHHHHHHHHHH
MLENVKLKPIFGEIMSRLEDGKKTQINDIVYLLQEENLISDFNDIIKAFYESENVTEQAV
HHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
DDIINKILINSLAVKREKIKRAINEAHMLGDIEKERQLLIELQNCEKEMLKIKNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]