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The map label for this gene is pyk [H]

Identifier: 167039840

GI number: 167039840

Start: 1232950

End: 1234701

Strand: Direct

Name: pyk [H]

Synonym: Teth514_1195

Alternate gene names: 167039840

Gene position: 1232950-1234701 (Clockwise)

Preceding gene: 167039839

Following gene: 167039841

Centisome position: 50.18

GC content: 36.59

Gene sequence:

>1752_bases
ATGCGCAGAACAAAAATAGTGTGTACAATAGGACCTGCCAGCGAAGACTATAATATACTGAGAAAATTAATTGAGAAAGG
TATGAACGTTGCTCGATTGAATTTTTCTCATGGGGATTTTGAAGAGCATGGGGCAAGAATTGACAATATTAAAAAGATAA
GAGAAGAATTCGGGCTACCTGTTGCTATTTTGTTGGATACTAAAGGTCCAGAGATAAGGACAGGAAAGTTTAAAAATGGC
GGAGTGGAATTAAAAGAAGGACAAACTTTTGTCATTACGACAAGAGATGTATTAGGAGACGAAACTATATGCAGTGTATC
CTATAAAGGGTTGCCTCAAGATGTGGAAAGAGGTAGCCGCATACTAATTGACGATGGGTTAATTTCTCTAAAGGTTACTG
ATGTTAAAGGAGAAGATATAGTTTGTATTGTAGAAAATTCTGGTCCTGTAAAAGACCATAAAGGAGTAAATGTTCCAGGA
GTAAAACTCAATCTTCCTGCTTTGACTCAAAAAGACGTAGATGATATAGAGTTTGGAATTCAAAAGGGCATTGATATAAT
TGCGGCTTCTTTTGTTAGAAAAGCAGCTGATGTTTTGGCAATAAGAAGACTTTTAGAAGAAAATAAGGCAGAGCATATTT
TAATTATTGCTAAAATAGAAAATCGCGAAGGAGTAGAAAATATAGACGAAATTATAAAGGTTTCAGATGGAATAATGGTA
GCCAGAGGAGATTTAGGGGTTGAGATTCCTCTTGAAGAAATACCAATCGTTCAAAAGATGATTATACAAAAATGTAATAA
AGCAGGGAAACCGGTAATTACTGCAACTCAAATGTTAGACTCTATGATGAGAAATCCAAGGCCTACAAGAGCAGAAGTTA
CAGATGTAGCAAACGCTATTCTAGATGGGACAGATGCTATAATGCTATCAGGGGAGACAGCTCAAGGTAAATACCCCATA
GAGGCCTTTGAGACAATGGCAAAGATTGCAGAAAAGACAGAAGCTTATGTGGGATACAGAGATATCATTGATAGAAATAT
TGATACAAATGTTTCTATTACAAACGCTATAAGCCATGCAACTTGTACTACTGCAAGAGATATAGGTGCAGCTGCTATAA
TAACTTGTACTAAATCTGGCTATACAGCGAGAATGGTATCAAGGTACAGACCTCAAGCTCCGATTATTGCTACTACTCCA
AGCGAAAGTGTTGCAAGGAAACTCTCTATAGTCTGGGGAGTATATCCTTTGATTACAGAAGAGGTTTCTACTACAGATGA
AATGATAGATGTCGCAATACAAAGTGCTTTAACTGCAGGACTTATAAGAAATGGTGATATCGTAGTAATTTCTGCAGGAA
TTCCTGTAGCTATGACAGGCACCACCAACATGTTGAAGGTACATATAGTAGGAGATGTTTTGTTGAGAGGAATAGGTATA
GGTTCTAAGGCTACTACAGGTGTTGTATGTGTTATTAATGATATCAGCAAAGACAAAGATAAATTTAGAGAAGGCGATAT
AATAGTAACTGCAAAGACAGAAAGAGATTTTATGCCTCTTATAGAGAAAGCTTCTGCTATAATCACAGAAGAAGGAGGGC
TTACTTCTCACGCAGCTATTGTGGGATTAAACTTAGGAATTCCTGTAGTTGTAGGTTGTGAAGGAGCGACTTCTAAATTA
AAAGATGGCATGACTGTAACGGTTGACACAGTTAGAGGATTTGTGTATAAAGGAATTGTAAATATTAAATAA

Upstream 100 bases:

>100_bases
GATATAGATGAAGCCCTTTCCATGAAAAAGGAGTTTAACAGAAAACTTTATGAGCTCAGCAAGATATTGTCTATATAAAT
AAATTTTAGGGAGTGAAGGT

Downstream 100 bases:

>100_bases
ATAAGACAAGATTTTCTCTTGTCTTTGTTTTTTTAAGGAGTGATTTAAATTATGCATGTATGGGATGCCGAGGTAAGGGC
TCGATACGGAGAAACGGACA

Product: pyruvate kinase., pyruvate, water dikinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREEFGLPVAILLDTKGPEIRTGKFKNG
GVELKEGQTFVITTRDVLGDETICSVSYKGLPQDVERGSRILIDDGLISLKVTDVKGEDIVCIVENSGPVKDHKGVNVPG
VKLNLPALTQKDVDDIEFGIQKGIDIIAASFVRKAADVLAIRRLLEENKAEHILIIAKIENREGVENIDEIIKVSDGIMV
ARGDLGVEIPLEEIPIVQKMIIQKCNKAGKPVITATQMLDSMMRNPRPTRAEVTDVANAILDGTDAIMLSGETAQGKYPI
EAFETMAKIAEKTEAYVGYRDIIDRNIDTNVSITNAISHATCTTARDIGAAAIITCTKSGYTARMVSRYRPQAPIIATTP
SESVARKLSIVWGVYPLITEEVSTTDEMIDVAIQSALTAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVLLRGIGI
GSKATTGVVCVINDISKDKDKFREGDIIVTAKTERDFMPLIEKASAIITEEGGLTSHAAIVGLNLGIPVVVGCEGATSKL
KDGMTVTVDTVRGFVYKGIVNIK

Sequences:

>Translated_583_residues
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREEFGLPVAILLDTKGPEIRTGKFKNG
GVELKEGQTFVITTRDVLGDETICSVSYKGLPQDVERGSRILIDDGLISLKVTDVKGEDIVCIVENSGPVKDHKGVNVPG
VKLNLPALTQKDVDDIEFGIQKGIDIIAASFVRKAADVLAIRRLLEENKAEHILIIAKIENREGVENIDEIIKVSDGIMV
ARGDLGVEIPLEEIPIVQKMIIQKCNKAGKPVITATQMLDSMMRNPRPTRAEVTDVANAILDGTDAIMLSGETAQGKYPI
EAFETMAKIAEKTEAYVGYRDIIDRNIDTNVSITNAISHATCTTARDIGAAAIITCTKSGYTARMVSRYRPQAPIIATTP
SESVARKLSIVWGVYPLITEEVSTTDEMIDVAIQSALTAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVLLRGIGI
GSKATTGVVCVINDISKDKDKFREGDIIVTAKTERDFMPLIEKASAIITEEGGLTSHAAIVGLNLGIPVVVGCEGATSKL
KDGMTVTVDTVRGFVYKGIVNIK
>Mature_583_residues
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREEFGLPVAILLDTKGPEIRTGKFKNG
GVELKEGQTFVITTRDVLGDETICSVSYKGLPQDVERGSRILIDDGLISLKVTDVKGEDIVCIVENSGPVKDHKGVNVPG
VKLNLPALTQKDVDDIEFGIQKGIDIIAASFVRKAADVLAIRRLLEENKAEHILIIAKIENREGVENIDEIIKVSDGIMV
ARGDLGVEIPLEEIPIVQKMIIQKCNKAGKPVITATQMLDSMMRNPRPTRAEVTDVANAILDGTDAIMLSGETAQGKYPI
EAFETMAKIAEKTEAYVGYRDIIDRNIDTNVSITNAISHATCTTARDIGAAAIITCTKSGYTARMVSRYRPQAPIIATTP
SESVARKLSIVWGVYPLITEEVSTTDEMIDVAIQSALTAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVLLRGIGI
GSKATTGVVCVINDISKDKDKFREGDIIVTAKTERDFMPLIEKASAIITEEGGLTSHAAIVGLNLGIPVVVGCEGATSKL
KDGMTVTVDTVRGFVYKGIVNIK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=45.5852156057495, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=45.5852156057495, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=45.9016393442623, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=45.7905544147844, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=45.7905544147844, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=51.0416666666667, Blast_Score=275, Evalue=1e-73,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=51.0416666666667, Blast_Score=275, Evalue=1e-73,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=48.3050847457627, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=48.3050847457627, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=47.906976744186, Blast_Score=186, Evalue=7e-47,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=50.4237288135593, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=40.2922755741127, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1787994, Length=119, Percent_Identity=42.8571428571429, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=46.3768115942029, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=44.6280991735537, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=44.6280991735537, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=44.6280991735537, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=44.6280991735537, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=44.3298969072165, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=42.0944558521561, Blast_Score=351, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24648964, Length=488, Percent_Identity=45.2868852459016, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28571814, Length=488, Percent_Identity=45.2868852459016, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24648966, Length=511, Percent_Identity=41.8786692759295, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24581235, Length=470, Percent_Identity=36.1702127659575, Blast_Score=288, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24646914, Length=285, Percent_Identity=44.5614035087719, Blast_Score=229, Evalue=4e-60,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62941; Mature: 62941

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREEFGLP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHCCC
VAILLDTKGPEIRTGKFKNGGVELKEGQTFVITTRDVLGDETICSVSYKGLPQDVERGSR
EEEEEECCCCCEECCCCCCCCEEEECCCEEEEEEHHHCCCCEEEEEECCCCCHHHHCCCE
ILIDDGLISLKVTDVKGEDIVCIVENSGPVKDHKGVNVPGVKLNLPALTQKDVDDIEFGI
EEEECCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
QKGIDIIAASFVRKAADVLAIRRLLEENKAEHILIIAKIENREGVENIDEIIKVSDGIMV
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEE
ARGDLGVEIPLEEIPIVQKMIIQKCNKAGKPVITATQMLDSMMRNPRPTRAEVTDVANAI
EECCCCEECCHHHCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHH
LDGTDAIMLSGETAQGKYPIEAFETMAKIAEKTEAYVGYRDIIDRNIDTNVSITNAISHA
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEHHHHHH
TCTTARDIGAAAIITCTKSGYTARMVSRYRPQAPIIATTPSESVARKLSIVWGVYPLITE
HHCCHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
EVSTTDEMIDVAIQSALTAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVLLRGIGI
HHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCEEEECCCCEEEEEEEHHHHHHHCCC
GSKATTGVVCVINDISKDKDKFREGDIIVTAKTERDFMPLIEKASAIITEEGGLTSHAAI
CCCCCCCEEEEEECCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHEEEECCCCCCCCEEE
VGLNLGIPVVVGCEGATSKLKDGMTVTVDTVRGFVYKGIVNIK
EEEECCCCEEEECCCCHHHHHCCCEEEEHHHHHHHHHCEEECC
>Mature Secondary Structure
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREEFGLP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHCCC
VAILLDTKGPEIRTGKFKNGGVELKEGQTFVITTRDVLGDETICSVSYKGLPQDVERGSR
EEEEEECCCCCEECCCCCCCCEEEECCCEEEEEEHHHCCCCEEEEEECCCCCHHHHCCCE
ILIDDGLISLKVTDVKGEDIVCIVENSGPVKDHKGVNVPGVKLNLPALTQKDVDDIEFGI
EEEECCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
QKGIDIIAASFVRKAADVLAIRRLLEENKAEHILIIAKIENREGVENIDEIIKVSDGIMV
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEE
ARGDLGVEIPLEEIPIVQKMIIQKCNKAGKPVITATQMLDSMMRNPRPTRAEVTDVANAI
EECCCCEECCHHHCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHH
LDGTDAIMLSGETAQGKYPIEAFETMAKIAEKTEAYVGYRDIIDRNIDTNVSITNAISHA
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEHHHHHH
TCTTARDIGAAAIITCTKSGYTARMVSRYRPQAPIIATTPSESVARKLSIVWGVYPLITE
HHCCHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
EVSTTDEMIDVAIQSALTAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVLLRGIGI
HHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCEEEECCCCEEEEEEEHHHHHHHCCC
GSKATTGVVCVINDISKDKDKFREGDIIVTAKTERDFMPLIEKASAIITEEGGLTSHAAI
CCCCCCCEEEEEECCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHEEEECCCCCCCCEEE
VGLNLGIPVVVGCEGATSKLKDGMTVTVDTVRGFVYKGIVNIK
EEEECCCCEEEECCCCHHHHHCCCEEEEHHHHHHHHHCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]