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The map label for this gene is pfkA

Identifier: 167039839

GI number: 167039839

Start: 1231962

End: 1232927

Strand: Direct

Name: pfkA

Synonym: Teth514_1194

Alternate gene names: 167039839

Gene position: 1231962-1232927 (Clockwise)

Preceding gene: 167039838

Following gene: 167039840

Centisome position: 50.14

GC content: 37.68

Gene sequence:

>966_bases
ATGAAGACGATTGGTATACTTACAAGTGGTGGAGATGCACCAGGGATGAACGCAGCTATAAGGGCTGTGGTAAGGACAGG
AATTTATTATGGGCTTAAAGTAAAAGGTATTATGAGGGGATATGCAGGCTTAGTAGAGGATGAAGTTATTGATTTAAATC
TTTCTTCAGTAGGAGATATATTGCAAAAAGGCGGTACTATTTTGAGAACTGCTAGATGTGAAGAATTTAAGAAAAAAGAG
GTGCGCAAAAAAGCTTACGAAACCCTTCAAAAACATGGAATAGAAGGTCTCGTTGTAATTGGAGGAGATGGCTCTTTTAG
AGGAGCACAGCTTTTGAGTGAAGAATGGAATGTAAATACAATTGGAATTCCAGGTACAATTGACAATGACATACCTTGTA
CAGATTATACAATAGGTTTTGATACAGCTTGTAATACTGTTATTGATGCAATAAATAAAATAAGGGATACTGCTACTTCC
CATGAGAGAGCAAATATAATAGAAGTGATGGGAAGAAATGCGGGTTACATTGCTTTGTATGCTGGTCTAGCTGGTGGAGC
GGAGATGATTATACTTCCTGAAGTGGAATGGAGCATCGATGAACTTTGCGATAAAATTACTTACGGGATAAAGAGAGGAA
AGCTTCATCATATAATTGTCCTTGCAGAAGGAGTTATGAGCGCTCCAGAACTAGCGAAAATGATTAAAGAAAGGCTACCA
AAATTGGACTTAAGGTATACTATTTTGGGGCACATTCAAAGAGGTGGAGCTCCTACAGTGATGGATAGGGTATTGGCAAG
CCAAATGGGAGCAAGAGCAGTGGAGTTGCTTTTAGAAAATAAAACCAAGAGGATAATAAGTATACGAAACAATCAAATTG
TAGACGATGATATAGATGAAGCCCTTTCCATGAAAAAGGAGTTTAACAGAAAACTTTATGAGCTCAGCAAGATATTGTCT
ATATAA

Upstream 100 bases:

>100_bases
ATTATTATTGAGTATGGCTTATAATTTTTTGCCCGAAAGGGCTGCTTTAACGAAAATGGGACAACTAAAGTCCATGTGGG
ATAAAATGAGGAGGTAGACT

Downstream 100 bases:

>100_bases
ATAAATTTTAGGGAGTGAAGGTATGCGCAGAACAAAAATAGTGTGTACAATAGGACCTGCCAGCGAAGACTATAATATAC
TGAGAAAATTAATTGAGAAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGYAGLVEDEVIDLNLSSVGDILQKGGTILRTARCEEFKKKE
VRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNTIGIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATS
HERANIIEVMGRNAGYIALYAGLAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRIISIRNNQIVDDDIDEALSMKKEFNRKLYELSKILS
I

Sequences:

>Translated_321_residues
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGYAGLVEDEVIDLNLSSVGDILQKGGTILRTARCEEFKKKE
VRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNTIGIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATS
HERANIIEVMGRNAGYIALYAGLAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRIISIRNNQIVDDDIDEALSMKKEFNRKLYELSKILS
I
>Mature_321_residues
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGYAGLVEDEVIDLNLSSVGDILQKGGTILRTARCEEFKKKE
VRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNTIGIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATS
HERANIIEVMGRNAGYIALYAGLAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRIISIRNNQIVDDDIDEALSMKKEFNRKLYELSKILS
I

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=350, Percent_Identity=41.4285714285714, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI48762920, Length=349, Percent_Identity=40.4011461318052, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI266453619, Length=351, Percent_Identity=38.1766381766382, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI266453768, Length=351, Percent_Identity=38.1766381766382, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI266453748, Length=351, Percent_Identity=38.1766381766382, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI4505749, Length=351, Percent_Identity=38.1766381766382, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=49.6875, Blast_Score=304, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI25147584, Length=332, Percent_Identity=41.2650602409639, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17558788, Length=314, Percent_Identity=37.5796178343949, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=309, Percent_Identity=28.1553398058252, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=38.2608695652174, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6321679, Length=342, Percent_Identity=38.5964912280702, Blast_Score=212, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24652337, Length=331, Percent_Identity=42.9003021148036, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI17647809, Length=331, Percent_Identity=42.9003021148036, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI28573326, Length=331, Percent_Identity=42.2960725075529, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_THEP3 (B0K7U7)

Other databases:

- EMBL:   CP000924
- RefSeq:   YP_001664682.1
- ProteinModelPortal:   B0K7U7
- SMR:   B0K7U7
- GeneID:   5874001
- GenomeReviews:   CP000924_GR
- KEGG:   tpd:Teth39_0683
- HOGENOM:   HBG405044
- OMA:   QKENINA
- ProtClustDB:   PRK03202
- BioCyc:   TPSE340099:TETH39_0683-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 35216; Mature: 35216

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 245-245 BINDING 251-251 BINDING 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGYAGLVEDEVIDLNLSSVGDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHCCCEEECCHHHHHHH
LQKGGTILRTARCEEFKKKEVRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNT
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEE
IGIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATSHERANIIEVMGRNAGYIALY
EECCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEE
AGLAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
ECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRIISIRNNQIVDDDIDE
CCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHH
ALSMKKEFNRKLYELSKILSI
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGYAGLVEDEVIDLNLSSVGDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHCCCEEECCHHHHHHH
LQKGGTILRTARCEEFKKKEVRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNT
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEE
IGIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATSHERANIIEVMGRNAGYIALY
EECCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEE
AGLAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
ECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRIISIRNNQIVDDDIDE
CCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHH
ALSMKKEFNRKLYELSKILSI
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA