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The map label for this gene is hisF

Identifier: 167039656

GI number: 167039656

Start: 1036036

End: 1036797

Strand: Direct

Name: hisF

Synonym: Teth514_1006

Alternate gene names: 167039656

Gene position: 1036036-1036797 (Clockwise)

Preceding gene: 167039655

Following gene: 167039657

Centisome position: 42.16

GC content: 38.19

Gene sequence:

>762_bases
ATGCTTGCAAAAAGAATAATTCCTTGTTTAGATGTGAAAGACGGCAGGGTAGTAAAAGGAGTTAACTTTTTAAATTTAAA
AGATGCGGGAGACCCTGTCCAGATAGCATCAGAGTACAATGAATTAGGAGCAGATGAGCTTGTATTCTTGGACATTACTG
CTTCTTATGAAAAACGAAAAATAATGATAGAAGTTGTAAAAAGGACTTCTGAAAAAGTATTTATTCCTTTAACTGTTGGT
GGTGGAATTTCAGACCTTGACGATATAAAAGATATTCTAAGAGCAGGGGCTGATAAGGTTTCAATAAATACCCAAGCTGT
GAAACAACCAACTCTTATTCGTCAGGCCGCTGTACGTTTTGGAAGCCAATGTGTGGTTGTAGCAATTGATGCCAAGAGGC
GAAGTGATGGCTCGGGCTTTGATGTGTATATAAACGGCGGAAGGGTAAACACAGGGCTTGATGTGATTGAGTGGGCTAAA
AAAGTAGAGTCTTTAGGAGCAGGAGAAATACTTCTTACAAGTATGGATAAAGACGGTACTAAAGATGGGTATGATATAGA
GCTTACAAGAATGGTAAGTGAAGCTGTCAATATCCCCGTTATAGCTTCTGGAGGTGCAGGAAATAAAGAGCATTTTAAAG
AGGTTTTTACTGAAGGCAAAGCTGATGCTGCGTTAGCGGCTTCTGTGTTTCATTACAGGGAGTTGGAAATAAAAGAAGTT
AAAAGATATTTAAAAGAAGAAGGTATACCAGTGAGAATTTAA

Upstream 100 bases:

>100_bases
TTTAAAAAAGCTTAAAGAGATGGGAGCATATGGTGCGATAATTGGAAAAGCCTTATATATTGGAAAAATAAACTTTAAAA
GTGCTTTGGAGGTAATATAA

Downstream 100 bases:

>100_bases
ATTTATCGAAAGGATGTAGAGAATGATTGATATAGAGGAATTAAAGTTTGATGAAAAAGGACTTATTCCTGCGATTGTGC
AAGACTACAACACAAAACAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLAKRIIPCLDVKDGRVVKGVNFLNLKDAGDPVQIASEYNELGADELVFLDITASYEKRKIMIEVVKRTSEKVFIPLTVG
GGISDLDDIKDILRAGADKVSINTQAVKQPTLIRQAAVRFGSQCVVVAIDAKRRSDGSGFDVYINGGRVNTGLDVIEWAK
KVESLGAGEILLTSMDKDGTKDGYDIELTRMVSEAVNIPVIASGGAGNKEHFKEVFTEGKADAALAASVFHYRELEIKEV
KRYLKEEGIPVRI

Sequences:

>Translated_253_residues
MLAKRIIPCLDVKDGRVVKGVNFLNLKDAGDPVQIASEYNELGADELVFLDITASYEKRKIMIEVVKRTSEKVFIPLTVG
GGISDLDDIKDILRAGADKVSINTQAVKQPTLIRQAAVRFGSQCVVVAIDAKRRSDGSGFDVYINGGRVNTGLDVIEWAK
KVESLGAGEILLTSMDKDGTKDGYDIELTRMVSEAVNIPVIASGGAGNKEHFKEVFTEGKADAALAASVFHYRELEIKEV
KRYLKEEGIPVRI
>Mature_253_residues
MLAKRIIPCLDVKDGRVVKGVNFLNLKDAGDPVQIASEYNELGADELVFLDITASYEKRKIMIEVVKRTSEKVFIPLTVG
GGISDLDDIKDILRAGADKVSINTQAVKQPTLIRQAAVRFGSQCVVVAIDAKRRSDGSGFDVYINGGRVNTGLDVIEWAK
KVESLGAGEILLTSMDKDGTKDGYDIELTRMVSEAVNIPVIASGGAGNKEHFKEVFTEGKADAALAASVFHYRELEIKEV
KRYLKEEGIPVRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=49.2248062015504, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI87082028, Length=207, Percent_Identity=28.9855072463768, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=38.8535031847134, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_THEPX (B0K629)

Other databases:

- EMBL:   CP000923
- RefSeq:   YP_001662641.1
- ProteinModelPortal:   B0K629
- SMR:   B0K629
- GeneID:   5877715
- GenomeReviews:   CP000923_GR
- KEGG:   tex:Teth514_1006
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   TSP1755:TETH514_1006-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27599; Mature: 27599

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGRVVKGVNFLNLKDAGDPVQIASEYNELGADELVFLDITASYEKRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
IMIEVVKRTSEKVFIPLTVGGGISDLDDIKDILRAGADKVSINTQAVKQPTLIRQAAVRF
HHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHHCCHHHHHHHHHHC
GSQCVVVAIDAKRRSDGSGFDVYINGGRVNTGLDVIEWAKKVESLGAGEILLTSMDKDGT
CCCEEEEEEECCCCCCCCCEEEEEECCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
KDGYDIELTRMVSEAVNIPVIASGGAGNKEHFKEVFTEGKADAALAASVFHYRELEIKEV
CCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHH
KRYLKEEGIPVRI
HHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVKDGRVVKGVNFLNLKDAGDPVQIASEYNELGADELVFLDITASYEKRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHH
IMIEVVKRTSEKVFIPLTVGGGISDLDDIKDILRAGADKVSINTQAVKQPTLIRQAAVRF
HHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHHCCHHHHHHHHHHC
GSQCVVVAIDAKRRSDGSGFDVYINGGRVNTGLDVIEWAKKVESLGAGEILLTSMDKDGT
CCCEEEEEEECCCCCCCCCEEEEEECCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
KDGYDIELTRMVSEAVNIPVIASGGAGNKEHFKEVFTEGKADAALAASVFHYRELEIKEV
CCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHH
KRYLKEEGIPVRI
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA