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The map label for this gene is mdeA [H]

Identifier: 167039628

GI number: 167039628

Start: 997040

End: 998323

Strand: Direct

Name: mdeA [H]

Synonym: Teth514_0976

Alternate gene names: 167039628

Gene position: 997040-998323 (Clockwise)

Preceding gene: 167039627

Following gene: 167039629

Centisome position: 40.58

GC content: 38.86

Gene sequence:

>1284_bases
ATGTCTAAAAAATATGGTTTTTCAACTTTGGCAATTCATGAAGGGTGGGAGCCTGACCCAACTACGGGAGCTGTAGCACT
TCCTATATATCAAACTTCTTCCTTTGCTTTTAAAAGTACACAACATGCTGCAGACCTCTTTGCCTTAAAAGAAGAAGGGT
ATATATATACTCGAATGATGAACCCTACTGTTGATGTTTTTGAAAAAAGAATAGCTGCATTAGAAGGTGGGGTAGGTGCC
CTTGCTGTATCTTCAGGTCAAGCAGCTATAACGCTGGCAGTTACTAATATCGCAGGAGTAGGAGACGAGATTGTATCTTC
TACCAATCTTTATGGAGGGACTTATAATCTTTTTGCGACTACTTTGAAAAAGTTTGGTATAAATTTTAAGTTTGTGGACC
CCAGCAATCCTGAAAACTTTAAAAGTCAAATAACAGATAACACAAGAGCTTTGTACGTTGAGACTATTGGCAATCCAAAG
ATTGACGTGGCTGACATTGAAAAAATAGCAGAAATTGCCCACACTGCAGGAATTCCTCTCATTGTAGATAATACTTTTGC
AACTCCTTACCTTGCACGGCCTATCGAGTTTGGGGCTGATATAGTGGTACATTCGGCGACTAAATTTATAGGTGGACATG
GGACTTCTATAGGTGGAGTTATAGTAGATTCTGGAAAATTTAATTGGGACAATGGAAAGTTTCCAGAACTTACAGAACCA
GATCCAAGTTATCATGGTATAAGGTATGTAAAAGATGTAGGCGAAGCAGCTTATATTGTAAAAGCGAGGGTGCAGCTTTT
AAGGGATTTGGGTACTGCTTTGAGTCCATTTAATGCATTTTTATTCGCACAAGGATTAGAGACTCTATCTCTTAGGATGG
AAAGGCACAGTCAAAATGCTTTAAAAGTTGCAGAGTTTTTAGCTTCTCATCCCTACGTTTCGTGGGTGAATTATCCAGGA
CTTAAATCAAGTCCCTATTATGCTTTGGCACAAAAGTATTTGCCAAGAGGGCAAGGAGGAGTTTTGACTTTTGGCATAAA
AGGCGGATTAAAAGCAGGCATTAAGTTTATAGAGAGTTTAAAATTATTTACTCATCTTGCTAATATAGGGGATGCAAAGT
CTTTGGTTATACATCCAGCCAGCACGACCCATCAGCAGTTATCAAAAGAAGAACAGTTAGCTTCTGGTGTTACGGAGGAT
ATGATAAGACTTTCTGTAGGGCTGGAAGACATAGAAGATATATTAGAAGACCTTGATAATGCCCTTTATGCATCGCAAGA
ATAG

Upstream 100 bases:

>100_bases
ACAAGAGGATATAGATGAGTTACAGTTAAAAGAATTTGAAGATTTGGTAGGAGTAATTTAGAAATCAAATATTTATATTT
GATGAAAGGGGATAAAGATT

Downstream 100 bases:

>100_bases
ATAAGGAAAGGTTGATGTAGATGATAGTTGAGGAAAAAAAGGTTACACTAAAAGGGAAAAATATATTCACTACAGAAAGT
GGATATAGCTTTGATGAACT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSKKYGFSTLAIHEGWEPDPTTGAVALPIYQTSSFAFKSTQHAADLFALKEEGYIYTRMMNPTVDVFEKRIAALEGGVGA
LAVSSGQAAITLAVTNIAGVGDEIVSSTNLYGGTYNLFATTLKKFGINFKFVDPSNPENFKSQITDNTRALYVETIGNPK
IDVADIEKIAEIAHTAGIPLIVDNTFATPYLARPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFNWDNGKFPELTEP
DPSYHGIRYVKDVGEAAYIVKARVQLLRDLGTALSPFNAFLFAQGLETLSLRMERHSQNALKVAEFLASHPYVSWVNYPG
LKSSPYYALAQKYLPRGQGGVLTFGIKGGLKAGIKFIESLKLFTHLANIGDAKSLVIHPASTTHQQLSKEEQLASGVTED
MIRLSVGLEDIEDILEDLDNALYASQE

Sequences:

>Translated_427_residues
MSKKYGFSTLAIHEGWEPDPTTGAVALPIYQTSSFAFKSTQHAADLFALKEEGYIYTRMMNPTVDVFEKRIAALEGGVGA
LAVSSGQAAITLAVTNIAGVGDEIVSSTNLYGGTYNLFATTLKKFGINFKFVDPSNPENFKSQITDNTRALYVETIGNPK
IDVADIEKIAEIAHTAGIPLIVDNTFATPYLARPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFNWDNGKFPELTEP
DPSYHGIRYVKDVGEAAYIVKARVQLLRDLGTALSPFNAFLFAQGLETLSLRMERHSQNALKVAEFLASHPYVSWVNYPG
LKSSPYYALAQKYLPRGQGGVLTFGIKGGLKAGIKFIESLKLFTHLANIGDAKSLVIHPASTTHQQLSKEEQLASGVTED
MIRLSVGLEDIEDILEDLDNALYASQE
>Mature_426_residues
SKKYGFSTLAIHEGWEPDPTTGAVALPIYQTSSFAFKSTQHAADLFALKEEGYIYTRMMNPTVDVFEKRIAALEGGVGAL
AVSSGQAAITLAVTNIAGVGDEIVSSTNLYGGTYNLFATTLKKFGINFKFVDPSNPENFKSQITDNTRALYVETIGNPKI
DVADIEKIAEIAHTAGIPLIVDNTFATPYLARPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFNWDNGKFPELTEPD
PSYHGIRYVKDVGEAAYIVKARVQLLRDLGTALSPFNAFLFAQGLETLSLRMERHSQNALKVAEFLASHPYVSWVNYPGL
KSSPYYALAQKYLPRGQGGVLTFGIKGGLKAGIKFIESLKLFTHLANIGDAKSLVIHPASTTHQQLSKEEQLASGVTEDM
IRLSVGLEDIEDILEDLDNALYASQE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=405, Percent_Identity=36.7901234567901, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI299473759, Length=405, Percent_Identity=33.3333333333333, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI25453487, Length=404, Percent_Identity=31.1881188118812, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1790375, Length=427, Percent_Identity=30.4449648711944, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789383, Length=419, Percent_Identity=24.8210023866348, Blast_Score=138, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=412, Percent_Identity=34.7087378640777, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17560056, Length=407, Percent_Identity=34.1523341523341, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17538055, Length=408, Percent_Identity=33.8235294117647, Blast_Score=214, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=53.5962877030162, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=36.8794326241135, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321254, Length=436, Percent_Identity=26.605504587156, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=365, Percent_Identity=27.6712328767123, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=431, Percent_Identity=26.6821345707657, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19922554, Length=422, Percent_Identity=37.2037914691943, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46293; Mature: 46162

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKYGFSTLAIHEGWEPDPTTGAVALPIYQTSSFAFKSTQHAADLFALKEEGYIYTRMM
CCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHEEECCCEEEEEEC
NPTVDVFEKRIAALEGGVGALAVSSGQAAITLAVTNIAGVGDEIVSSTNLYGGTYNLFAT
CCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHCCCCCCCHHHHHHH
TLKKFGINFKFVDPSNPENFKSQITDNTRALYVETIGNPKIDVADIEKIAEIAHTAGIPL
HHHHHCCEEEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCE
IVDNTFATPYLARPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFNWDNGKFPELTEP
EEECCCCCCHHCCCHHCCCCEEEEHHHHHHCCCCCCCCCEEEECCEECCCCCCCCCCCCC
DPSYHGIRYVKDVGEAAYIVKARVQLLRDLGTALSPFNAFLFAQGLETLSLRMERHSQNA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LKVAEFLASHPYVSWVNYPGLKSSPYYALAQKYLPRGQGGVLTFGIKGGLKAGIKFIESL
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
KLFTHLANIGDAKSLVIHPASTTHQQLSKEEQLASGVTEDMIRLSVGLEDIEDILEDLDN
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
ALYASQE
HHHCCCC
>Mature Secondary Structure 
SKKYGFSTLAIHEGWEPDPTTGAVALPIYQTSSFAFKSTQHAADLFALKEEGYIYTRMM
CCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHEEECCCEEEEEEC
NPTVDVFEKRIAALEGGVGALAVSSGQAAITLAVTNIAGVGDEIVSSTNLYGGTYNLFAT
CCHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHCCCCCCCHHHHHHH
TLKKFGINFKFVDPSNPENFKSQITDNTRALYVETIGNPKIDVADIEKIAEIAHTAGIPL
HHHHHCCEEEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCE
IVDNTFATPYLARPIEFGADIVVHSATKFIGGHGTSIGGVIVDSGKFNWDNGKFPELTEP
EEECCCCCCHHCCCHHCCCCEEEEHHHHHHCCCCCCCCCEEEECCEECCCCCCCCCCCCC
DPSYHGIRYVKDVGEAAYIVKARVQLLRDLGTALSPFNAFLFAQGLETLSLRMERHSQNA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LKVAEFLASHPYVSWVNYPGLKSSPYYALAQKYLPRGQGGVLTFGIKGGLKAGIKFIESL
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
KLFTHLANIGDAKSLVIHPASTTHQQLSKEEQLASGVTEDMIRLSVGLEDIEDILEDLDN
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
ALYASQE
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]