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The map label for this gene is truA

Identifier: 167039553

GI number: 167039553

Start: 922400

End: 923134

Strand: Direct

Name: truA

Synonym: Teth514_0900

Alternate gene names: 167039553

Gene position: 922400-923134 (Clockwise)

Preceding gene: 167039552

Following gene: 167039554

Centisome position: 37.54

GC content: 33.33

Gene sequence:

>735_bases
ATGAGAAACGTAATGATTGTAGTAGAATATGACGGGACCAATTATCACGGTTGGCAATACCAAAAGAATGCAGTGACAGT
TCAGGAGGTATTGCAAAAAGCAATAAAGAAGGTAACAGGGGAAGAAGTTAATTTAATAGGTGCGAGTAGAACTGATACAG
GTGTCCATGCTCTATATCAAGTAGCTAACTTTAAAACTAATACTAAAATACCTGCAGAAAAGCTGCCCTATGCTCTAAAC
AGTGTATTACCCGATGATATAGTAGTTGTCCAAGCAAAGGATGTGGAAGACTCTTTTCACGCTCGATATAGTGCAAAAAG
GAAAAGATACAAATACATTATTCTTAATAGGAAATTTCAAATGCCCACAATGAGAAATTATTGTTGGCATATAAGTTATC
CTTTAAATATAGAAGAAATGAAAAAAGCTGCTTCTTACTTAATTGGGACTCACGATTTTTCTGCTTTTAAAGCTTCAGGA
AGTAGTAAAACAAGTACTATAAGGACAGTGTATGATTTAACTATTGAAAAAAATGAAGATTTTATCAACATTGAAATAGA
AGCCAATGGTTTTTTGTATAACATGGTGCGAATAATTGTAGGTACACTGTCTTATGTAGGTCTTGGCAAAATAAAAGAAG
ACGAAGTTTATGATATTTTAAAATCAAAAGACAGAACAAAAGCGGGCATAACTGCCCCGCCACAAGGATTGTATTTAATA
AAAATTATCTATTGA

Upstream 100 bases:

>100_bases
TGAAACAGCTTAAAATCCAACCGAGAGATTATCAAGCTTTAGCTGTGACCTTGATAATGGTATTGCTAATTGTATGGAAT
AGAGTTTGGACGTGGTAAAA

Downstream 100 bases:

>100_bases
CAGTTGCAGTACTGTGAATTAAAATAATATTATGCGAAATTGTGCCCTGTGCTGGAAATTTACCACGGATAAGGAGGTTA
TATAGAAGATGAAGTCATAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRNVMIVVEYDGTNYHGWQYQKNAVTVQEVLQKAIKKVTGEEVNLIGASRTDTGVHALYQVANFKTNTKIPAEKLPYALN
SVLPDDIVVVQAKDVEDSFHARYSAKRKRYKYIILNRKFQMPTMRNYCWHISYPLNIEEMKKAASYLIGTHDFSAFKASG
SSKTSTIRTVYDLTIEKNEDFINIEIEANGFLYNMVRIIVGTLSYVGLGKIKEDEVYDILKSKDRTKAGITAPPQGLYLI
KIIY

Sequences:

>Translated_244_residues
MRNVMIVVEYDGTNYHGWQYQKNAVTVQEVLQKAIKKVTGEEVNLIGASRTDTGVHALYQVANFKTNTKIPAEKLPYALN
SVLPDDIVVVQAKDVEDSFHARYSAKRKRYKYIILNRKFQMPTMRNYCWHISYPLNIEEMKKAASYLIGTHDFSAFKASG
SSKTSTIRTVYDLTIEKNEDFINIEIEANGFLYNMVRIIVGTLSYVGLGKIKEDEVYDILKSKDRTKAGITAPPQGLYLI
KIIY
>Mature_244_residues
MRNVMIVVEYDGTNYHGWQYQKNAVTVQEVLQKAIKKVTGEEVNLIGASRTDTGVHALYQVANFKTNTKIPAEKLPYALN
SVLPDDIVVVQAKDVEDSFHARYSAKRKRYKYIILNRKFQMPTMRNYCWHISYPLNIEEMKKAASYLIGTHDFSAFKASG
SSKTSTIRTVYDLTIEKNEDFINIEIEANGFLYNMVRIIVGTLSYVGLGKIKEDEVYDILKSKDRTKAGITAPPQGLYLI
KIIY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI199559785, Length=270, Percent_Identity=25.1851851851852, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1788657, Length=241, Percent_Identity=37.7593360995851, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71985046, Length=247, Percent_Identity=27.1255060728745, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6321112, Length=231, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=28.0528052805281, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_THEP3 (B0KCN3)

Other databases:

- EMBL:   CP000924
- RefSeq:   YP_001664412.1
- ProteinModelPortal:   B0KCN3
- SMR:   B0KCN3
- GeneID:   5874116
- GenomeReviews:   CP000924_GR
- KEGG:   tpd:Teth39_0407
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   TPSE340099:TETH39_0407-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27867; Mature: 27867

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVMIVVEYDGTNYHGWQYQKNAVTVQEVLQKAIKKVTGEEVNLIGASRTDTGVHALYQ
CCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
VANFKTNTKIPAEKLPYALNSVLPDDIVVVQAKDVEDSFHARYSAKRKRYKYIILNRKFQ
HHCCCCCCCCCHHHCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEEECEEC
MPTMRNYCWHISYPLNIEEMKKAASYLIGTHDFSAFKASGSSKTSTIRTVYDLTIEKNED
CCCCCCCEEEEECCCCHHHHHHHHHHEECCCCCHHEECCCCCCCCEEEEEEEEEEECCCC
FINIEIEANGFLYNMVRIIVGTLSYVGLGKIKEDEVYDILKSKDRTKAGITAPPQGLYLI
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEE
KIIY
EEEC
>Mature Secondary Structure
MRNVMIVVEYDGTNYHGWQYQKNAVTVQEVLQKAIKKVTGEEVNLIGASRTDTGVHALYQ
CCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
VANFKTNTKIPAEKLPYALNSVLPDDIVVVQAKDVEDSFHARYSAKRKRYKYIILNRKFQ
HHCCCCCCCCCHHHCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEEECEEC
MPTMRNYCWHISYPLNIEEMKKAASYLIGTHDFSAFKASGSSKTSTIRTVYDLTIEKNED
CCCCCCCEEEEECCCCHHHHHHHHHHEECCCCCHHEECCCCCCCCEEEEEEEEEEECCCC
FINIEIEANGFLYNMVRIIVGTLSYVGLGKIKEDEVYDILKSKDRTKAGITAPPQGLYLI
EEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEE
KIIY
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA