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The map label for this gene is kinE [H]

Identifier: 167039448

GI number: 167039448

Start: 827610

End: 828992

Strand: Direct

Name: kinE [H]

Synonym: Teth514_0794

Alternate gene names: 167039448

Gene position: 827610-828992 (Clockwise)

Preceding gene: 167039447

Following gene: 167039449

Centisome position: 33.68

GC content: 29.72

Gene sequence:

>1383_bases
ATGAAAGTGAACAAAGCTTTTGCTACAGATAAAAATTCCTTGCAGTGGATATGCAACCTTTTAGATAATTCACACGATTT
TTTTGTCTTAAAAGATTTAAATGGTAAGGTAGTTCATGTAAATAAAAGACTTCTTGAAACATTAGGCTATACCGTTGATG
AGTTAGATAATTATGATTATTTTTTTTACCATATATTAAAATACAAAGAAGTAGGACCGTACGAAGATGGTCTTATCATA
GAGCTTACGGCAAAATACAATATAAGAATGTCTTTTAGCATAAAATTTAGTCCTATAGCTAACAGTGTTGGTGATAAAAT
AGCTTTTTTAGGGTTTGTGAAGTTGCGAAGCTATTGTAAAGAAAAACCTGAAGATTATTTTAACTTCTTCAAACATCCAA
ATGACGTAATGGATATGACAGAGGAAGGTGTTGTGATAGTTGATAATAGGTTTAACGTGGTCTATGTGAATAACAGGACA
TGTGAATTATTTGGCATTAAATATACAAAATTCATAAATGCAAAGTTTATGGACATTATAAAAAAATACAAACCGGATGC
TTCAGAAAATAATTTAATTAACGGAGAAAAAATTGTAATCCCATTGAATGGGAGTTTTGACGAAAGAGTGCTTTTGCTTA
AGTATGGGAAGTTAAACAGTGGGCATTGGAAAACTATTATTGTGAAAAATATAACTGAAGACTTAAAATATCAAAAATTA
ATTGAGCAAACGGAAAAATACACAATTGCTGGTGAATTTGCTGCCACAACCATTCATGAAATAAAAAATTCTTTAACTTC
TATAAAAGGCTTTATACAACTTCTACAAGCTAAACATAAAGAAAGTTCTACTTACTATGAGACTATTTTAGATGAAGTGG
ACAGAATATTAGCTTTAATAAAAAATTACCTTGGTATAGTTAGAAGCAATGAAGAAGGAGAAACTGAAATAGACATAAAT
GGCGTTATAAATCAATATTTACTTCTTTTTGAAGCAGAAGCTGTTCGCAAAAAAATTAAAATAGAAAAAAGGTTTGGCGA
TGTCCCTCCTATAAAAATGGACAAAAATCATCTTAAGCAGCTTATTCTAAATGTAGTTCAAAATTCTCTTCATGCCATTG
GGGAGAATGGTAAAATAATATTAGATACAAGATATTACACTCTCAAAAAAAGGGTGGTAATAAGAATTGTTGATAATGGA
GGTGGTATAGAGAAGAAATATTTAAAAAAAGTTATTGAGCCCTTGTTTACAACTAAAAGAGAAGGAACAGGCCTGGGACT
TGCAATTTGCAAAAGTATTGTAGAATTGTACAATGGTGATATTAAAATTTTTAGTAAAAAAGGCAAAGGTACACTTGTAA
AAATAATTTTAGGTGAGAGATAA

Upstream 100 bases:

>100_bases
TTATTGACAAAAAGGAGGATTTTTCTACTTTAATGTCTAATTATATTAGTGGGACTAAGGCTTTTGAACGAAAGATAAGG
GAGATAAAAGGGGGACAGTC

Downstream 100 bases:

>100_bases
AAATAAAAGCTGGAGGGGAAAATTTATGAAATCTGATATTGAAATAGCTCAAGAAGCGAAAATGCTACATATAAGGGAGG
TTGCAAAAAAGTTAGACATT

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKVNKAFATDKNSLQWICNLLDNSHDFFVLKDLNGKVVHVNKRLLETLGYTVDELDNYDYFFYHILKYKEVGPYEDGLII
ELTAKYNIRMSFSIKFSPIANSVGDKIAFLGFVKLRSYCKEKPEDYFNFFKHPNDVMDMTEEGVVIVDNRFNVVYVNNRT
CELFGIKYTKFINAKFMDIIKKYKPDASENNLINGEKIVIPLNGSFDERVLLLKYGKLNSGHWKTIIVKNITEDLKYQKL
IEQTEKYTIAGEFAATTIHEIKNSLTSIKGFIQLLQAKHKESSTYYETILDEVDRILALIKNYLGIVRSNEEGETEIDIN
GVINQYLLLFEAEAVRKKIKIEKRFGDVPPIKMDKNHLKQLILNVVQNSLHAIGENGKIILDTRYYTLKKRVVIRIVDNG
GGIEKKYLKKVIEPLFTTKREGTGLGLAICKSIVELYNGDIKIFSKKGKGTLVKIILGER

Sequences:

>Translated_460_residues
MKVNKAFATDKNSLQWICNLLDNSHDFFVLKDLNGKVVHVNKRLLETLGYTVDELDNYDYFFYHILKYKEVGPYEDGLII
ELTAKYNIRMSFSIKFSPIANSVGDKIAFLGFVKLRSYCKEKPEDYFNFFKHPNDVMDMTEEGVVIVDNRFNVVYVNNRT
CELFGIKYTKFINAKFMDIIKKYKPDASENNLINGEKIVIPLNGSFDERVLLLKYGKLNSGHWKTIIVKNITEDLKYQKL
IEQTEKYTIAGEFAATTIHEIKNSLTSIKGFIQLLQAKHKESSTYYETILDEVDRILALIKNYLGIVRSNEEGETEIDIN
GVINQYLLLFEAEAVRKKIKIEKRFGDVPPIKMDKNHLKQLILNVVQNSLHAIGENGKIILDTRYYTLKKRVVIRIVDNG
GGIEKKYLKKVIEPLFTTKREGTGLGLAICKSIVELYNGDIKIFSKKGKGTLVKIILGER
>Mature_460_residues
MKVNKAFATDKNSLQWICNLLDNSHDFFVLKDLNGKVVHVNKRLLETLGYTVDELDNYDYFFYHILKYKEVGPYEDGLII
ELTAKYNIRMSFSIKFSPIANSVGDKIAFLGFVKLRSYCKEKPEDYFNFFKHPNDVMDMTEEGVVIVDNRFNVVYVNNRT
CELFGIKYTKFINAKFMDIIKKYKPDASENNLINGEKIVIPLNGSFDERVLLLKYGKLNSGHWKTIIVKNITEDLKYQKL
IEQTEKYTIAGEFAATTIHEIKNSLTSIKGFIQLLQAKHKESSTYYETILDEVDRILALIKNYLGIVRSNEEGETEIDIN
GVINQYLLLFEAEAVRKKIKIEKRFGDVPPIKMDKNHLKQLILNVVQNSLHAIGENGKIILDTRYYTLKKRVVIRIVDNG
GGIEKKYLKKVIEPLFTTKREGTGLGLAICKSIVELYNGDIKIFSKKGKGTLVKIILGER

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=348, Percent_Identity=29.0229885057471, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=32.1428571428571, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790300, Length=337, Percent_Identity=24.3323442136499, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=26.7241379310345, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52951; Mature: 52951

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNKAFATDKNSLQWICNLLDNSHDFFVLKDLNGKVVHVNKRLLETLGYTVDELDNYDY
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECHHHHHHHCCCHHHCCCCCE
FFYHILKYKEVGPYEDGLIIELTAKYNIRMSFSIKFSPIANSVGDKIAFLGFVKLRSYCK
EEHEEEHHHCCCCCCCCEEEEEEEEEEEEEEEEEEECCHHHHHCCHHHHHHHHHHHHHHH
EKPEDYFNFFKHPNDVMDMTEEGVVIVDNRFNVVYVNNRTCELFGIKYTKFINAKFMDII
CCCHHHHHHHCCCCHHHHHCCCCEEEEECCEEEEEEECCEEEEEEEEHHHHHHHHHHHHH
KKYKPDASENNLINGEKIVIPLNGSFDERVLLLKYGKLNSGHWKTIIVKNITEDLKYQKL
HHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEEEEEEHHHHHHHHHHH
IEQTEKYTIAGEFAATTIHEIKNSLTSIKGFIQLLQAKHKESSTYYETILDEVDRILALI
HHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KNYLGIVRSNEEGETEIDINGVINQYLLLFEAEAVRKKIKIEKRFGDVPPIKMDKNHLKQ
HHHHHEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LILNVVQNSLHAIGENGKIILDTRYYTLKKRVVIRIVDNGGGIEKKYLKKVIEPLFTTKR
HHHHHHHHHHHHCCCCCEEEEEECEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCC
EGTGLGLAICKSIVELYNGDIKIFSKKGKGTLVKIILGER
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCC
>Mature Secondary Structure
MKVNKAFATDKNSLQWICNLLDNSHDFFVLKDLNGKVVHVNKRLLETLGYTVDELDNYDY
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECHHHHHHHCCCHHHCCCCCE
FFYHILKYKEVGPYEDGLIIELTAKYNIRMSFSIKFSPIANSVGDKIAFLGFVKLRSYCK
EEHEEEHHHCCCCCCCCEEEEEEEEEEEEEEEEEEECCHHHHHCCHHHHHHHHHHHHHHH
EKPEDYFNFFKHPNDVMDMTEEGVVIVDNRFNVVYVNNRTCELFGIKYTKFINAKFMDII
CCCHHHHHHHCCCCHHHHHCCCCEEEEECCEEEEEEECCEEEEEEEEHHHHHHHHHHHHH
KKYKPDASENNLINGEKIVIPLNGSFDERVLLLKYGKLNSGHWKTIIVKNITEDLKYQKL
HHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEEEEEEHHHHHHHHHHH
IEQTEKYTIAGEFAATTIHEIKNSLTSIKGFIQLLQAKHKESSTYYETILDEVDRILALI
HHHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KNYLGIVRSNEEGETEIDINGVINQYLLLFEAEAVRKKIKIEKRFGDVPPIKMDKNHLKQ
HHHHHEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LILNVVQNSLHAIGENGKIILDTRYYTLKKRVVIRIVDNGGGIEKKYLKKVIEPLFTTKR
HHHHHHHHHHHHCCCCCEEEEEECEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCC
EGTGLGLAICKSIVELYNGDIKIFSKKGKGTLVKIILGER
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]