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The map label for this gene is ogt [H]

Identifier: 167039287

GI number: 167039287

Start: 655251

End: 655721

Strand: Direct

Name: ogt [H]

Synonym: Teth514_0628

Alternate gene names: 167039287

Gene position: 655251-655721 (Clockwise)

Preceding gene: 167039285

Following gene: 167039288

Centisome position: 26.67

GC content: 36.31

Gene sequence:

>471_bases
GTGCTATATAAAAAATTTATCTCTCCTGTTGGTGTTCTCACTATTTTTTCATCAGGAAAAGGTATATGCCGCATTGACTT
TGAAAATGAGAAATCTTCATTAGAAGGTTTTAAAGAAGGCAGCGATTTTTATATTGAAGAGTGTATTAGGCAGCTTGACC
TATATTTTCAAAAGAAATTAAAAAATTTTGACGTGCGGTTGGATTTGCAAGGTACAGAGTTTCAGAAGGCCGTGTGGAAG
GAGATTTTAAAAATTCCTTATGGTGAAGTTAAGACATATGGAGAGATTGCGAGATTAATTGGAAAGCCAAAGGCGGCAAG
AGCAGTAGGGCAGGCTCTTAATAAGAATCCTATTCCCATTATTATCCCTTGCCACAGAGTCATTGGAAGCAGTGAAGATT
TGACAGGCTTTGGCGGGGGAATTGAAATAAAAAAATTTTTATTAAGACATGAAGGAGTAGAGGTTCATTAA

Upstream 100 bases:

>100_bases
TAGCCTGAAACGATTACTCAAAAAATTTTACAATATTTTTTCTTTTCATATTGTGTTATAATTAATTTACCAGTTTTTAT
AGATTCAAGGAGATGAGACT

Downstream 100 bases:

>100_bases
AAATTTATGTTTCATGGAAAGTAAGGTGATGAGGATGGATTATTTAAAAATTTTAAAGGAGTTTTTTAAAAATAAGGACA
ATATAGTTATGGCTTTTTTA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MLYKKFISPVGVLTIFSSGKGICRIDFENEKSSLEGFKEGSDFYIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQKAVWK
EILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEGVEVH

Sequences:

>Translated_156_residues
MLYKKFISPVGVLTIFSSGKGICRIDFENEKSSLEGFKEGSDFYIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQKAVWK
EILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEGVEVH
>Mature_156_residues
MLYKKFISPVGVLTIFSSGKGICRIDFENEKSSLEGFKEGSDFYIEECIRQLDLYFQKKLKNFDVRLDLQGTEFQKAVWK
EILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPIIIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEGVEVH

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=161, Percent_Identity=34.7826086956522, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=48.5436893203884, Blast_Score=104, Evalue=3e-24,
Organism=Escherichia coli, GI1788542, Length=152, Percent_Identity=32.8947368421053, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1786659, Length=82, Percent_Identity=37.8048780487805, Blast_Score=61, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=48.8095238095238, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=48.8095238095238, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=48.1927710843374, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17635; Mature: 17635

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYKKFISPVGVLTIFSSGKGICRIDFENEKSSLEGFKEGSDFYIEECIRQLDLYFQKKL
CCHHHHHCHHHHEEEECCCCCEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KNFDVRLDLQGTEFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPI
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
IIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEGVEVH
EEECHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLYKKFISPVGVLTIFSSGKGICRIDFENEKSSLEGFKEGSDFYIEECIRQLDLYFQKKL
CCHHHHHCHHHHEEEECCCCCEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KNFDVRLDLQGTEFQKAVWKEILKIPYGEVKTYGEIARLIGKPKAARAVGQALNKNPIPI
CCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
IIPCHRVIGSSEDLTGFGGGIEIKKFLLRHEGVEVH
EEECHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]