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The map label for this gene is rph

Identifier: 167039210

GI number: 167039210

Start: 572517

End: 573302

Strand: Direct

Name: rph

Synonym: Teth514_0551

Alternate gene names: 167039210

Gene position: 572517-573302 (Clockwise)

Preceding gene: 167039209

Following gene: 167039211

Centisome position: 23.3

GC content: 36.9

Gene sequence:

>786_bases
ATGAACAGGATTGATGGAAGAGAATTTAATGAACTGAGACCTATAAAAATTACAAGAAATTTTAATAAGTTTGCTGAAGG
TTCAGTGCTAATTGAGATGGGAAACACAAAAGTCATTTGCACTGCCTCCATTGAGGATAAGGTACCTCCATTTCAAAAAG
GTACAGGTAAGGGTTGGATAACCAGTGAGTATGGTATGTTGCCAAGAGCAACTGAAACAAGGAATCCAAGAGAAGTAACT
AAAGGAAGACCTAGTGGAAGGACTATGGAAATACAGCGGCTTATAGGCCGTTCTTTAAGGTCTGTAGTGGATTTGGATGT
GTTAGGAGAAAAGACTATATGGATAGATTGCGATGTTATACAAGCAGATGGGGGTACAAGAACAGCTTCTATTACAGGCT
CATTTATAGCATTGGCAGATGCACTTAACAAATTGGTAGAAAAAGGTGACATCCCTAAGATTCCTTTGAAAGGTTTTGTT
GCGGCTGTAAGTGTAGGAATTGTGGAGGGAAATGAGCTTTTAGATTTATCCTTTCAAGAAGATTCGAATGCATTAGTAGA
TATGAATGTAGTTATGACAGACAAAGGAGAAATTGTAGAAATACAGGGAACAGGTGAAGGTGGACCATTTACAAAGCAGA
ATTTGACAGACCTTTTAAGCCTTGCAGAATATGGGATTGAACAGATTATCAAAATTCAAAAAGAAGTTCTTTCAGATATT
GTAGATAAAATAGGAGTTGATTCAGTTGAAAATAATAATAGCAACCCACAATCCTCACAAAACTGA

Upstream 100 bases:

>100_bases
AAATATTAATAAAGGTTTTAAAATAATATTGAGCGATAAAAACAATAAAGCAGAATAAATTAACTTTAAACTATTTTTAT
GAAATGAAAGGGTTGAAGAA

Downstream 100 bases:

>100_bases
AGAAATAAAAAATTTTTTTAAAGGCTATCCTGTTGAAATTTATTCTATGGCAGACTTGGGTATAAAAGAAGACATAGAAG
AGACAGGAGATACGATTGAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGNTKVICTASIEDKVPPFQKGTGKGWITSEYGMLPRATETRNPREVT
KGRPSGRTMEIQRLIGRSLRSVVDLDVLGEKTIWIDCDVIQADGGTRTASITGSFIALADALNKLVEKGDIPKIPLKGFV
AAVSVGIVEGNELLDLSFQEDSNALVDMNVVMTDKGEIVEIQGTGEGGPFTKQNLTDLLSLAEYGIEQIIKIQKEVLSDI
VDKIGVDSVENNNSNPQSSQN

Sequences:

>Translated_261_residues
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGNTKVICTASIEDKVPPFQKGTGKGWITSEYGMLPRATETRNPREVT
KGRPSGRTMEIQRLIGRSLRSVVDLDVLGEKTIWIDCDVIQADGGTRTASITGSFIALADALNKLVEKGDIPKIPLKGFV
AAVSVGIVEGNELLDLSFQEDSNALVDMNVVMTDKGEIVEIQGTGEGGPFTKQNLTDLLSLAEYGIEQIIKIQKEVLSDI
VDKIGVDSVENNNSNPQSSQN
>Mature_261_residues
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGNTKVICTASIEDKVPPFQKGTGKGWITSEYGMLPRATETRNPREVT
KGRPSGRTMEIQRLIGRSLRSVVDLDVLGEKTIWIDCDVIQADGGTRTASITGSFIALADALNKLVEKGDIPKIPLKGFV
AAVSVGIVEGNELLDLSFQEDSNALVDMNVVMTDKGEIVEIQGTGEGGPFTKQNLTDLLSLAEYGIEQIIKIQKEVLSDI
VDKIGVDSVENNNSNPQSSQN

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Homo sapiens, GI9506689, Length=186, Percent_Identity=29.5698924731183, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=65.2380952380952, Blast_Score=287, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI71981632, Length=193, Percent_Identity=31.0880829015544, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_THEP3 (B0KBM5)

Other databases:

- EMBL:   CP000924
- RefSeq:   YP_001665656.1
- ProteinModelPortal:   B0KBM5
- SMR:   B0KBM5
- GeneID:   5874623
- GenomeReviews:   CP000924_GR
- KEGG:   tpd:Teth39_1683
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   TPSE340099:TETH39_1683-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 28496; Mature: 28496

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGNTKVICTASIEDKVPPFQKGTGKGWI
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCCEE
TSEYGMLPRATETRNPREVTKGRPSGRTMEIQRLIGRSLRSVVDLDVLGEKTIWIDCDVI
ECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
QADGGTRTASITGSFIALADALNKLVEKGDIPKIPLKGFVAAVSVGIVEGNELLDLSFQE
ECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCEEEEEEECC
DSNALVDMNVVMTDKGEIVEIQGTGEGGPFTKQNLTDLLSLAEYGIEQIIKIQKEVLSDI
CCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKIGVDSVENNNSNPQSSQN
HHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRIDGREFNELRPIKITRNFNKFAEGSVLIEMGNTKVICTASIEDKVPPFQKGTGKGWI
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCCEE
TSEYGMLPRATETRNPREVTKGRPSGRTMEIQRLIGRSLRSVVDLDVLGEKTIWIDCDVI
ECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
QADGGTRTASITGSFIALADALNKLVEKGDIPKIPLKGFVAAVSVGIVEGNELLDLSFQE
ECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCEEEEEEECC
DSNALVDMNVVMTDKGEIVEIQGTGEGGPFTKQNLTDLLSLAEYGIEQIIKIQKEVLSDI
CCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKIGVDSVENNNSNPQSSQN
HHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA