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The map label for this gene is purF [H]

Identifier: 167039181

GI number: 167039181

Start: 537659

End: 539056

Strand: Direct

Name: purF [H]

Synonym: Teth514_0522

Alternate gene names: 167039181

Gene position: 537659-539056 (Clockwise)

Preceding gene: 167039180

Following gene: 167039182

Centisome position: 21.88

GC content: 35.55

Gene sequence:

>1398_bases
ATGGGAAATGGAGAGAAATTAAAAGAGGAATGTGGTGTTTTTGGTGCCTTTAGTTTGTCCACTTCAGTTACTTCTTATAT
CTATTATGGCTTACAAGCCTTGCAGCACAGAGGGCAGGAAAGTTCAGGTATAGCTATTTATGATGGCGAAAAAATAAATT
GTATTAAGGGGTTGGGACTTGTCAGCGAGGTCTTTAATAAGGAAAATTTAAAGACATTAGAGGGAAAAATGGGGATAGGC
CATGTAAGGTATTCTACTACAGGCAGCAATGACATTATAAATGCTCAACCCCTCGTTGCTAATTTTAAAAATGAATATAT
GGCTCTTGCCCATAATGGCAATTTGATAAACGCAAAAGAGTTAAGGGGGCAATTGGAGGAGGACGGTAGAATATTTCAAA
CTACAACAGATAGCGAAATAATTCTTCATTTAATTGCAAAAAATTTCCAAAAGGGCTTAATAGAAGCCCTCAAGGAAACA
ATAAAACAAATAAAAGGTTCATATGCTTTGGTGATTTTAGCAGCCAATAAATTAATAGGGATAAGAGATGTAAACAGTAT
AAGGCCTCTTTGTATAGGTAAAAAAGATGACACTTATTTCTTATCTTCTGAGTCTTGTGCCTTTGACGTAATAGGAGCAG
AGTTGATACGAGATGTAGAAGCAGGAGAAATAGTTATAATTGATGGAAAAGGAATAGATTCTGTTAAGTTAGAAGTAGAA
GAAAAAAAGATGCCTTGTGTTTTTGAATATATCTATTTTGCTAGACCTGACAGTGTCATAGATGAAAAAAGTGTTTATTT
TACACGATTAGAGATGGGGAAAAGATTAGCTGAAGAAGCACCTGTAGATGCAGACTTGGTAGTACCAGTTCCAGATTCAG
GTGTTGCTGCTGCAAGAGGTTATTCTCTCAAAACAGGTATACCAATGGGGGAAGGACTTATAAAAAATAAATACATTGGA
AGAACTTTTATTGCTCCTGACCAAAGGGATAGAGAGACAGGGGTGAGAATAAAGTTAAATGTTTTAAAAGAATTGATACA
AGGCAAAAGGATAGTTTTAATAGATGATTCAATTGTTAGAGGTACTACTATGAAGAGATTGGTGAATCTATTAAAAAATG
GGGGAGCAAAAGAGGTTCATGTGAGGATAAGTTCACCTCCTGTTAAATATTCCTGCTACTTTGGAATTGATACCCCGACA
AAAAAAGAATTGATAGCGGCGAGGATGTCTGTTGAAGAGATATGCAAAGTAATAGGGGCTGACAGCTTACAGTTTTTAAG
CATTGAAGGGCTTATAAAAAGTGTAGGGTTTAAATCTATTTGCACAGGTTGCTTTGATGACGACTATCCTATGTATGTTC
CAAAAGAAGGGAGTAAGTATCTTTTTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
GAGTTGTAGAAGGCAAAAAGATTGCAATTGAGGTAAATGGTAAAAAAATAATAGACCTTCCTTTAGAAGTCTTAGAGGAA
AGCTGGAGAGGGAGAATAAA

Downstream 100 bases:

>100_bases
AAAGTAGGTGTATAATTATGAATTATAAAGATGCTGGGGTAAATATAGATGAAGGCAATAAACTGGTGAAAATGATAAAA
CCTATTGCAAAAACGACTTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGLVSEVFNKENLKTLEGKMGIG
HVRYSTTGSNDIINAQPLVANFKNEYMALAHNGNLINAKELRGQLEEDGRIFQTTTDSEIILHLIAKNFQKGLIEALKET
IKQIKGSYALVILAANKLIGIRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDGKGIDSVKLEVE
EKKMPCVFEYIYFARPDSVIDEKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGVAAARGYSLKTGIPMGEGLIKNKYIG
RTFIAPDQRDRETGVRIKLNVLKELIQGKRIVLIDDSIVRGTTMKRLVNLLKNGGAKEVHVRISSPPVKYSCYFGIDTPT
KKELIAARMSVEEICKVIGADSLQFLSIEGLIKSVGFKSICTGCFDDDYPMYVPKEGSKYLFEKK

Sequences:

>Translated_465_residues
MGNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGLVSEVFNKENLKTLEGKMGIG
HVRYSTTGSNDIINAQPLVANFKNEYMALAHNGNLINAKELRGQLEEDGRIFQTTTDSEIILHLIAKNFQKGLIEALKET
IKQIKGSYALVILAANKLIGIRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDGKGIDSVKLEVE
EKKMPCVFEYIYFARPDSVIDEKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGVAAARGYSLKTGIPMGEGLIKNKYIG
RTFIAPDQRDRETGVRIKLNVLKELIQGKRIVLIDDSIVRGTTMKRLVNLLKNGGAKEVHVRISSPPVKYSCYFGIDTPT
KKELIAARMSVEEICKVIGADSLQFLSIEGLIKSVGFKSICTGCFDDDYPMYVPKEGSKYLFEKK
>Mature_464_residues
GNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGLVSEVFNKENLKTLEGKMGIGH
VRYSTTGSNDIINAQPLVANFKNEYMALAHNGNLINAKELRGQLEEDGRIFQTTTDSEIILHLIAKNFQKGLIEALKETI
KQIKGSYALVILAANKLIGIRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDGKGIDSVKLEVEE
KKMPCVFEYIYFARPDSVIDEKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGVAAARGYSLKTGIPMGEGLIKNKYIGR
TFIAPDQRDRETGVRIKLNVLKELIQGKRIVLIDDSIVRGTTMKRLVNLLKNGGAKEVHVRISSPPVKYSCYFGIDTPTK
KELIAARMSVEEICKVIGADSLQFLSIEGLIKSVGFKSICTGCFDDDYPMYVPKEGSKYLFEKK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=462, Percent_Identity=42.8571428571429, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI4826742, Length=219, Percent_Identity=29.6803652968037, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI205277386, Length=217, Percent_Identity=26.7281105990783, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788651, Length=451, Percent_Identity=40.7982261640798, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790167, Length=246, Percent_Identity=29.6747967479675, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=40.5857740585774, Blast_Score=334, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI17532899, Length=216, Percent_Identity=30.5555555555556, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17539970, Length=223, Percent_Identity=30.4932735426009, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17532897, Length=216, Percent_Identity=30.5555555555556, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323958, Length=451, Percent_Identity=40.1330376940133, Blast_Score=296, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6322745, Length=212, Percent_Identity=31.1320754716981, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28573187, Length=503, Percent_Identity=41.1530815109344, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=40.5737704918033, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=41.2017167381974, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21357745, Length=182, Percent_Identity=27.4725274725275, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51330; Mature: 51199

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGL
CCCHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEHHHHHHH
VSEVFNKENLKTLEGKMGIGHVRYSTTGSNDIINAQPLVANFKNEYMALAHNGNLINAKE
HHHHHCCCCCEECCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCEEEEEECCCEEEHHH
LRGQLEEDGRIFQTTTDSEIILHLIAKNFQKGLIEALKETIKQIKGSYALVILAANKLIG
HHCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEE
IRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDGKGIDSVKLEVE
EECCCCCCCEEECCCCCEEEECCCCCEEHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEE
EKKMPCVFEYIYFARPDSVIDEKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGVAAARG
CCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCC
YSLKTGIPMGEGLIKNKYIGRTFIAPDQRDRETGVRIKLNVLKELIQGKRIVLIDDSIVR
CEEECCCCCCCCHHHCCCCCCEECCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCHHC
GTTMKRLVNLLKNGGAKEVHVRISSPPVKYSCYFGIDTPTKKELIAARMSVEEICKVIGA
CHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHCC
DSLQFLSIEGLIKSVGFKSICTGCFDDDYPMYVPKEGSKYLFEKK
CCEEEEEHHHHHHHCCHHHHHHCCCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
GNGEKLKEECGVFGAFSLSTSVTSYIYYGLQALQHRGQESSGIAIYDGEKINCIKGLGL
CCHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEHHHHHHH
VSEVFNKENLKTLEGKMGIGHVRYSTTGSNDIINAQPLVANFKNEYMALAHNGNLINAKE
HHHHHCCCCCEECCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCEEEEEECCCEEEHHH
LRGQLEEDGRIFQTTTDSEIILHLIAKNFQKGLIEALKETIKQIKGSYALVILAANKLIG
HHCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEE
IRDVNSIRPLCIGKKDDTYFLSSESCAFDVIGAELIRDVEAGEIVIIDGKGIDSVKLEVE
EECCCCCCCEEECCCCCEEEECCCCCEEHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEE
EKKMPCVFEYIYFARPDSVIDEKSVYFTRLEMGKRLAEEAPVDADLVVPVPDSGVAAARG
CCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCC
YSLKTGIPMGEGLIKNKYIGRTFIAPDQRDRETGVRIKLNVLKELIQGKRIVLIDDSIVR
CEEECCCCCCCCHHHCCCCCCEECCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCHHC
GTTMKRLVNLLKNGGAKEVHVRISSPPVKYSCYFGIDTPTKKELIAARMSVEEICKVIGA
CHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHCC
DSLQFLSIEGLIKSVGFKSICTGCFDDDYPMYVPKEGSKYLFEKK
CCEEEEEHHHHHHHCCHHHHHHCCCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]