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The map label for this gene is leuC2 [H]

Identifier: 167039077

GI number: 167039077

Start: 427419

End: 429338

Strand: Direct

Name: leuC2 [H]

Synonym: Teth514_0416

Alternate gene names: 167039077

Gene position: 427419-429338 (Clockwise)

Preceding gene: 167039076

Following gene: 167039078

Centisome position: 17.39

GC content: 40.36

Gene sequence:

>1920_bases
ATGAATCTTACGCAGAAAATACTATCTTCTCACCTCATCGAAGGAGAAATGGTGAAAGGTCAAGAAATAGCCATTAAAAT
TGACCAAACTTTGACTCAAGACTCCACTGGTACAATGGCGTACCTACAGTTAGAAGCTCTTGGAATAGATAGGGTAAAAA
CTGAACTTTCGGTTGCTTACATTGACCACAATACATTGCAGCAAGGTCCTGAAAATGCTGATGACCACAAATACATCCAG
ACTGTTGCAGCAAAATACGGCATATATTTTTCAAGACCGGGCAATGGAATTTGCCATCAGGTACATCTCGAAAGATTCGG
AAGGCCTGGGAAAACTCTTTTAGGTTCTGATAGCCATACGCCAACAGCAGGTGGGTTAGGAATGTTAGCTATAGGAGCAG
GTGGATTAGATGTTGCTTTGGCTATGGCGGGAGAGCCTTACAGGATTATTATGCCTTCCATTGTCAATGTAAGGCTTACT
GGGAAACTTAGACCTTGGGTATCGGCAAAGGATGTAATATTGGAGCTTTTAAGGAGACTGACTGTAAAGGGTGGCGTTGG
GAAGATTTTTGAATATTCAGGGGATGGGGTAAAAACCCTATCAGTGCCCGAAAGAGCCACAATTGCTAATATGGGCGCGG
AATTAGGGGCTACTACATCAATTTTTCCATCGGATGAAAGGACGTATGAATTTTTAAAAGCCCAAGGAAGAGAAGAGGTA
TTTGTGCCTTTGGCTGCCGATGAAGATGCAGAATACGATGAGGTCATTGAAATAAACCTTGATGAACTGGAACCTTTAGT
AGCACTTCCTCATAGCCCTGATAATGTTGTAAAAGTTAAAGACGCAGGAAGGCCAAAAGTTGACCAAGTTGCTATAGGGT
CCTGCACAAATTCCTCTTATGCTGATTTGATGAAAGTTGCTTCCATATTGAGAGGCAAGGTTATTCTAGAACATGTAAGT
CTTGTAATTGCTCCAGGTTCAAGACAAGTTCTTAACATGCTTGCAAAAAATGGTGCACTTTCAGACCTGATATCGGCAGG
TGCAAGAATACTCGAAAGCGCTTGTGGCCCCTGTATTGGTATGGGGCAAGCCCCTGCAACAGGTGCAATTTCGATTAGAA
CCTTTAATAGAAACTTTTACGGCAGAAGTGGTACAAAGTCGGCATCAGTATACCTAGTAAGCCCTGAAGTTGCTGCTGCT
GCAGCTTTGACAGGGTATTTAATAGATCCGAGAGAGTTAGGGGAAGCACCTGAAGTACCTTATGTGGAGAAATTTGAAAT
TAATGACAATATGATTATAAAACCGCCAGAGGATAGGGAAAAGGTGGAGATAATAAAAGGACCTAATATAAAGCCATTCC
CTCTCAATACACCTCTTTCTGATATAGAAAAGAGGGTACTTATTAAAGTAGGAGATGACATAACTACAGACCATATAATG
CCTTCAAATGCTAAGTTGTTGCCCTTAAGGTCTAATATCCCTGAACTTGCTAAACATTGCTTCGAAATAATAGATGAAAA
CTTTTCTAAGAGAGCGATTGAATGGGGCGGTGGCATAATTGTCGGAGGAAGCAATTACGGACAAGGCTCCAGCCGAGAGC
ACGCAGCATTGGCTCCGCTTCAACTGGGAGTAAAGGCTGTCATAGCAAAGTCATTTGCGAGAATACACAAAGCAAACCTT
ATAAATAGTGGAATTCTTCCTCTTACTTTTGTAGATGAAAAAGATTATGAAGACATTGAGGTTGGGGATGTTTTAAAGAT
AGAAAATGCTGTAGAACAAGTAAGAAGTGGTAAAAAAATCATAATAGTAAATGTCACAAAAGGAAAAACATTTGAAGTAA
CTCTTGATGTTTCGGATAGAAACAGAGAGATATTAATAGCAGGCGGTATGATAAATTTTGTAAAAAGTAAAAAGAAATAA

Upstream 100 bases:

>100_bases
TAAGAGGCCGCTTTTTGACAAAGAGCTTATGTACCTTTATGAGGATGTTATCAAAAACAGAGAAAAGGCTATATAAAATT
ATGTTACAGGAGTGAAGGCA

Downstream 100 bases:

>100_bases
AACAAAGGTACTTGCTTTAAAGTAAGTGCTTTAGAGTAACAACAAACCCCACTTTTTATGAGCTTAAAAAGTGGGGTTTA
GTTTGTATTTGGATGTAATT

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MNLTQKILSSHLIEGEMVKGQEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVAYIDHNTLQQGPENADDHKYIQ
TVAAKYGIYFSRPGNGICHQVHLERFGRPGKTLLGSDSHTPTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPSIVNVRLT
GKLRPWVSAKDVILELLRRLTVKGGVGKIFEYSGDGVKTLSVPERATIANMGAELGATTSIFPSDERTYEFLKAQGREEV
FVPLAADEDAEYDEVIEINLDELEPLVALPHSPDNVVKVKDAGRPKVDQVAIGSCTNSSYADLMKVASILRGKVILEHVS
LVIAPGSRQVLNMLAKNGALSDLISAGARILESACGPCIGMGQAPATGAISIRTFNRNFYGRSGTKSASVYLVSPEVAAA
AALTGYLIDPRELGEAPEVPYVEKFEINDNMIIKPPEDREKVEIIKGPNIKPFPLNTPLSDIEKRVLIKVGDDITTDHIM
PSNAKLLPLRSNIPELAKHCFEIIDENFSKRAIEWGGGIIVGGSNYGQGSSREHAALAPLQLGVKAVIAKSFARIHKANL
INSGILPLTFVDEKDYEDIEVGDVLKIENAVEQVRSGKKIIIVNVTKGKTFEVTLDVSDRNREILIAGGMINFVKSKKK

Sequences:

>Translated_639_residues
MNLTQKILSSHLIEGEMVKGQEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVAYIDHNTLQQGPENADDHKYIQ
TVAAKYGIYFSRPGNGICHQVHLERFGRPGKTLLGSDSHTPTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPSIVNVRLT
GKLRPWVSAKDVILELLRRLTVKGGVGKIFEYSGDGVKTLSVPERATIANMGAELGATTSIFPSDERTYEFLKAQGREEV
FVPLAADEDAEYDEVIEINLDELEPLVALPHSPDNVVKVKDAGRPKVDQVAIGSCTNSSYADLMKVASILRGKVILEHVS
LVIAPGSRQVLNMLAKNGALSDLISAGARILESACGPCIGMGQAPATGAISIRTFNRNFYGRSGTKSASVYLVSPEVAAA
AALTGYLIDPRELGEAPEVPYVEKFEINDNMIIKPPEDREKVEIIKGPNIKPFPLNTPLSDIEKRVLIKVGDDITTDHIM
PSNAKLLPLRSNIPELAKHCFEIIDENFSKRAIEWGGGIIVGGSNYGQGSSREHAALAPLQLGVKAVIAKSFARIHKANL
INSGILPLTFVDEKDYEDIEVGDVLKIENAVEQVRSGKKIIIVNVTKGKTFEVTLDVSDRNREILIAGGMINFVKSKKK
>Mature_639_residues
MNLTQKILSSHLIEGEMVKGQEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVAYIDHNTLQQGPENADDHKYIQ
TVAAKYGIYFSRPGNGICHQVHLERFGRPGKTLLGSDSHTPTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPSIVNVRLT
GKLRPWVSAKDVILELLRRLTVKGGVGKIFEYSGDGVKTLSVPERATIANMGAELGATTSIFPSDERTYEFLKAQGREEV
FVPLAADEDAEYDEVIEINLDELEPLVALPHSPDNVVKVKDAGRPKVDQVAIGSCTNSSYADLMKVASILRGKVILEHVS
LVIAPGSRQVLNMLAKNGALSDLISAGARILESACGPCIGMGQAPATGAISIRTFNRNFYGRSGTKSASVYLVSPEVAAA
AALTGYLIDPRELGEAPEVPYVEKFEINDNMIIKPPEDREKVEIIKGPNIKPFPLNTPLSDIEKRVLIKVGDDITTDHIM
PSNAKLLPLRSNIPELAKHCFEIIDENFSKRAIEWGGGIIVGGSNYGQGSSREHAALAPLQLGVKAVIAKSFARIHKANL
INSGILPLTFVDEKDYEDIEVGDVLKIENAVEQVRSGKKIIIVNVTKGKTFEVTLDVSDRNREILIAGGMINFVKSKKK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=723, Percent_Identity=34.4398340248963, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI8659555, Length=723, Percent_Identity=27.6625172890733, Blast_Score=216, Evalue=5e-56,
Organism=Homo sapiens, GI41352693, Length=718, Percent_Identity=26.8802228412256, Blast_Score=188, Evalue=1e-47,
Organism=Escherichia coli, GI87081781, Length=726, Percent_Identity=29.8898071625344, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1787531, Length=606, Percent_Identity=28.5478547854785, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=28.5407725321888, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI2367097, Length=457, Percent_Identity=28.8840262582057, Blast_Score=93, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25149337, Length=727, Percent_Identity=35.2132049518569, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI32564738, Length=688, Percent_Identity=35.7558139534884, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI25149342, Length=574, Percent_Identity=35.191637630662, Blast_Score=276, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17568399, Length=799, Percent_Identity=26.4080100125156, Blast_Score=198, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323335, Length=733, Percent_Identity=34.106412005457, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6322261, Length=740, Percent_Identity=34.5945945945946, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6320440, Length=611, Percent_Identity=30.7692307692308, Blast_Score=224, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6321429, Length=661, Percent_Identity=26.928895612708, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI281365315, Length=719, Percent_Identity=34.9095966620306, Blast_Score=339, Evalue=4e-93,
Organism=Drosophila melanogaster, GI17864292, Length=719, Percent_Identity=34.9095966620306, Blast_Score=339, Evalue=4e-93,
Organism=Drosophila melanogaster, GI28571643, Length=727, Percent_Identity=33.837689133425, Blast_Score=331, Evalue=1e-90,
Organism=Drosophila melanogaster, GI161076999, Length=683, Percent_Identity=35.4319180087848, Blast_Score=329, Evalue=4e-90,
Organism=Drosophila melanogaster, GI17137564, Length=730, Percent_Identity=26.7123287671233, Blast_Score=172, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24645686, Length=728, Percent_Identity=26.9230769230769, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69130; Mature: 69130

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTQKILSSHLIEGEMVKGQEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVAY
CCHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCCEEEEEEEECCCHHHHEEEEEEE
IDHNTLQQGPENADDHKYIQTVAAKYGIYFSRPGNGICHQVHLERFGRPGKTLLGSDSHT
EECHHHHCCCCCCCHHHHHHHHHHHHCEEEECCCCCCEEEHHHHHCCCCCCEEECCCCCC
PTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPSIVNVRLTGKLRPWVSAKDVILELLRRL
CCCCCCEEEEECCCCCEEEEEECCCCEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHH
TVKGGVGKIFEYSGDGVKTLSVPERATIANMGAELGATTSIFPSDERTYEFLKAQGREEV
HCCCCCCEEEEECCCCEEEECCCCHHHHHHCCHHCCCCEEECCCCHHHHHHHHHCCCCEE
FVPLAADEDAEYDEVIEINLDELEPLVALPHSPDNVVKVKDAGRPKVDQVAIGSCTNSSY
EEEEECCCCCCCCEEEEEEHHHCCCEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCHH
ADLMKVASILRGKVILEHVSLVIAPGSRQVLNMLAKNGALSDLISAGARILESACGPCIG
HHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
MGQAPATGAISIRTFNRNFYGRSGTKSASVYLVSPEVAAAAALTGYLIDPRELGEAPEVP
CCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHCEEECHHHHCCCCCCC
YVEKFEINDNMIIKPPEDREKVEIIKGPNIKPFPLNTPLSDIEKRVLIKVGDDITTDHIM
CEEEEECCCCEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCC
PSNAKLLPLRSNIPELAKHCFEIIDENFSKRAIEWGGGIIVGGSNYGQGSSREHAALAPL
CCCCEEEEECCCCHHHHHHHHHHHHCCCHHCEEECCCEEEECCCCCCCCCCCCCCEECHH
QLGVKAVIAKSFARIHKANLINSGILPLTFVDEKDYEDIEVGDVLKIENAVEQVRSGKKI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHCCCEE
IIVNVTKGKTFEVTLDVSDRNREILIAGGMINFVKSKKK
EEEEECCCCEEEEEEEECCCCCEEEEECCHHHHHHCCCC
>Mature Secondary Structure
MNLTQKILSSHLIEGEMVKGQEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVAY
CCHHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCCCEEEEEEEECCCHHHHEEEEEEE
IDHNTLQQGPENADDHKYIQTVAAKYGIYFSRPGNGICHQVHLERFGRPGKTLLGSDSHT
EECHHHHCCCCCCCHHHHHHHHHHHHCEEEECCCCCCEEEHHHHHCCCCCCEEECCCCCC
PTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPSIVNVRLTGKLRPWVSAKDVILELLRRL
CCCCCCEEEEECCCCCEEEEEECCCCEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHH
TVKGGVGKIFEYSGDGVKTLSVPERATIANMGAELGATTSIFPSDERTYEFLKAQGREEV
HCCCCCCEEEEECCCCEEEECCCCHHHHHHCCHHCCCCEEECCCCHHHHHHHHHCCCCEE
FVPLAADEDAEYDEVIEINLDELEPLVALPHSPDNVVKVKDAGRPKVDQVAIGSCTNSSY
EEEEECCCCCCCCEEEEEEHHHCCCEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCHH
ADLMKVASILRGKVILEHVSLVIAPGSRQVLNMLAKNGALSDLISAGARILESACGPCIG
HHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
MGQAPATGAISIRTFNRNFYGRSGTKSASVYLVSPEVAAAAALTGYLIDPRELGEAPEVP
CCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHCEEECHHHHCCCCCCC
YVEKFEINDNMIIKPPEDREKVEIIKGPNIKPFPLNTPLSDIEKRVLIKVGDDITTDHIM
CEEEEECCCCEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCC
PSNAKLLPLRSNIPELAKHCFEIIDENFSKRAIEWGGGIIVGGSNYGQGSSREHAALAPL
CCCCEEEEECCCCHHHHHHHHHHHHCCCHHCEEECCCEEEECCCCCCCCCCCCCCEECHH
QLGVKAVIAKSFARIHKANLINSGILPLTFVDEKDYEDIEVGDVLKIENAVEQVRSGKKI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHCCCEE
IIVNVTKGKTFEVTLDVSDRNREILIAGGMINFVKSKKK
EEEEECCCCEEEEEEEECCCCCEEEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]