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The map label for this gene is aksA [H]

Identifier: 167039076

GI number: 167039076

Start: 426243

End: 427394

Strand: Direct

Name: aksA [H]

Synonym: Teth514_0415

Alternate gene names: 167039076

Gene position: 426243-427394 (Clockwise)

Preceding gene: 167039075

Following gene: 167039077

Centisome position: 17.35

GC content: 38.11

Gene sequence:

>1152_bases
ATGACTCTTAAAAAGGGTAAGAAAGTATACATTGTTGACACAACTTTAAGGGATGGAGAGCAAACTGCAGGAGTTGTGTT
TGCAAATAATGAAAAAATCAGAATTGCGCAAATGTTAGATGAAATTGGAATAGATCAATTAGAAGTAGGTATTCCCACAA
TGGGAGGAGACGAAAAAGAAACAGTTACAAAAATTGCAAAGCTTGGATTGAATGCCAGCATAATGGCGTGGAATAGAGCG
GTTGTAAAAGATGTTCAAGAATCTTTAGAATGTGGTGTTGATGCTGTTGCAATCTCTGTGTCTACTTCGGATATTCACAT
TGAGCACAAGCTTAAAAAGACAAGGCAATGGGTTTTGGACAATATGACTGAAGCAGTGAAATTTGCTAAAAAAGAAGGGG
TATATGTTTCTGTCAACGCAGAAGATGCATCTCGTACAGATATGAACTTCCTTATTGAATTTGCGAAATGTGCAAAACAA
GCAGGAGCCGATCGTTTGAGATTTTGCGACACCGTAGGATTTTTGGATCCTTTTAAAACATATGATATGGTCAAAGCAAT
AAAAGAAGCTGTTGACATTGATATTGAAATGCATACCCACAACGACTTTGGAATGGCTACTGCAAATGCTCTTGCGGGTA
TGAGAGCAGGAGCTAATTTTATAGGAGTTACTGTAAATGGCCTTGGCGAAAGAGCCGGAAATGCTGCTTTAGAAGAGGTT
GTAATGGCATTAAAGCACGTCTACAAAATAGATTTAGGAATAGATACAACTAGATTTAGGGAAATTTCCGAATACGTTGC
TTTAGCCTCAGGAAGACAGTTGCCAGCATGGAAAGCTATAGTTGGAACAAATGTCTTTGCCCATGAGTCAGGTATTCATG
TAGATGGTGCCCTTAAAAATCCTCACACATATGAAATATTTAACCCTGATGAAGTAGGCCTTGAAAGGCAGATTGTGATA
GGCAAGCATTCTGGCACTGCGGCTCTTATAAATAAGTTTAAAGAATACGGCAGAGTTTTAACAGAAGAAGAAGCAAATTT
GCTTTTGCCGCATGTCAGAAAATTAGCTATACAGCTTAAGAGGCCGCTTTTTGACAAAGAGCTTATGTACCTTTATGAGG
ATGTTATCAAAAACAGAGAAAAGGCTATATAA

Upstream 100 bases:

>100_bases
GTTTTGCAATTTTTATAATCTAAAATTGCAAAATACTATTGACTTTAAGAGGAGATACATATAAAATATGTTATAAAGAG
TTTTTGAAGGGGGAAGTTCG

Downstream 100 bases:

>100_bases
AATTATGTTACAGGAGTGAAGGCAATGAATCTTACGCAGAAAATACTATCTTCTCACCTCATCGAAGGAGAAATGGTGAA
AGGTCAAGAAATAGCCATTA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTLKKGKKVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKETVTKIAKLGLNASIMAWNRA
VVKDVQESLECGVDAVAISVSTSDIHIEHKLKKTRQWVLDNMTEAVKFAKKEGVYVSVNAEDASRTDMNFLIEFAKCAKQ
AGADRLRFCDTVGFLDPFKTYDMVKAIKEAVDIDIEMHTHNDFGMATANALAGMRAGANFIGVTVNGLGERAGNAALEEV
VMALKHVYKIDLGIDTTRFREISEYVALASGRQLPAWKAIVGTNVFAHESGIHVDGALKNPHTYEIFNPDEVGLERQIVI
GKHSGTAALINKFKEYGRVLTEEEANLLLPHVRKLAIQLKRPLFDKELMYLYEDVIKNREKAI

Sequences:

>Translated_383_residues
MTLKKGKKVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKETVTKIAKLGLNASIMAWNRA
VVKDVQESLECGVDAVAISVSTSDIHIEHKLKKTRQWVLDNMTEAVKFAKKEGVYVSVNAEDASRTDMNFLIEFAKCAKQ
AGADRLRFCDTVGFLDPFKTYDMVKAIKEAVDIDIEMHTHNDFGMATANALAGMRAGANFIGVTVNGLGERAGNAALEEV
VMALKHVYKIDLGIDTTRFREISEYVALASGRQLPAWKAIVGTNVFAHESGIHVDGALKNPHTYEIFNPDEVGLERQIVI
GKHSGTAALINKFKEYGRVLTEEEANLLLPHVRKLAIQLKRPLFDKELMYLYEDVIKNREKAI
>Mature_382_residues
TLKKGKKVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKETVTKIAKLGLNASIMAWNRAV
VKDVQESLECGVDAVAISVSTSDIHIEHKLKKTRQWVLDNMTEAVKFAKKEGVYVSVNAEDASRTDMNFLIEFAKCAKQA
GADRLRFCDTVGFLDPFKTYDMVKAIKEAVDIDIEMHTHNDFGMATANALAGMRAGANFIGVTVNGLGERAGNAALEEVV
MALKHVYKIDLGIDTTRFREISEYVALASGRQLPAWKAIVGTNVFAHESGIHVDGALKNPHTYEIFNPDEVGLERQIVIG
KHSGTAALINKFKEYGRVLTEEEANLLLPHVRKLAIQLKRPLFDKELMYLYEDVIKNREKAI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=288, Percent_Identity=24.3055555555556, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786261, Length=378, Percent_Identity=37.5661375661376, Blast_Score=210, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6320071, Length=370, Percent_Identity=32.1621621621622, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6320019, Length=354, Percent_Identity=32.2033898305085, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24582381, Length=286, Percent_Identity=25.8741258741259, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42439; Mature: 42308

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKKGKKVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKE
CCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCHHH
TVTKIAKLGLNASIMAWNRAVVKDVQESLECGVDAVAISVSTSDIHIEHKLKKTRQWVLD
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEHHHHHHHHHHHHHH
NMTEAVKFAKKEGVYVSVNAEDASRTDMNFLIEFAKCAKQAGADRLRFCDTVGFLDPFKT
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHH
YDMVKAIKEAVDIDIEMHTHNDFGMATANALAGMRAGANFIGVTVNGLGERAGNAALEEV
HHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
VMALKHVYKIDLGIDTTRFREISEYVALASGRQLPAWKAIVGTNVFAHESGIHVDGALKN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCEECCCCCC
PHTYEIFNPDEVGLERQIVIGKHSGTAALINKFKEYGRVLTEEEANLLLPHVRKLAIQLK
CCEEEEECCHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RPLFDKELMYLYEDVIKNREKAI
CCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLKKGKKVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKE
CCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCHHH
TVTKIAKLGLNASIMAWNRAVVKDVQESLECGVDAVAISVSTSDIHIEHKLKKTRQWVLD
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEHHHHHHHHHHHHHH
NMTEAVKFAKKEGVYVSVNAEDASRTDMNFLIEFAKCAKQAGADRLRFCDTVGFLDPFKT
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHH
YDMVKAIKEAVDIDIEMHTHNDFGMATANALAGMRAGANFIGVTVNGLGERAGNAALEEV
HHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
VMALKHVYKIDLGIDTTRFREISEYVALASGRQLPAWKAIVGTNVFAHESGIHVDGALKN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCEECCCCCC
PHTYEIFNPDEVGLERQIVIGKHSGTAALINKFKEYGRVLTEEEANLLLPHVRKLAIQLK
CCEEEEECCHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RPLFDKELMYLYEDVIKNREKAI
CCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]