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The map label for this gene is nagE [H]

Identifier: 167039073

GI number: 167039073

Start: 423268

End: 424668

Strand: Direct

Name: nagE [H]

Synonym: Teth514_0412

Alternate gene names: 167039073

Gene position: 423268-424668 (Clockwise)

Preceding gene: 167039072

Following gene: 167039074

Centisome position: 17.23

GC content: 39.54

Gene sequence:

>1401_bases
ATGAAGTGGTTAGCAAGTGTTCAAAAACTTGGTAAAGCCTTAATGCTTCCAGTTGCAGTTTTACCTGCAGCAGCATTGCT
TTTAAGGCTTGGAGCACCTGATGTTTTCAACATTCCTTTTATCATGCAGGCAGGTGCAGCGGTATTTGACAATTTACCCC
TCATCTTTGCTATAGGTATTGCAATAGGTTTTGCAGAAGGGGACGGAGTTGCGGCTCTTGCTGCAGCGGTAGGCTATTTT
GTACTGACAAAAGGTGCTACCACAATAAACAAAGACATCAATATGGGTGTTTTAGGCGGTATATTGATGGGTATCATCGC
AGGTTATTTGTATAACAAATACCATGATATTAAACTTCCTGATTTCTTAGGTTTCTTTGGAGGAAAGAGATTTGTTCCAA
TTGTAACGGCATTTGCTGCAATTGTACTGGCACTTGTGATGGGGTATGTATGGCCTCCAATACAGAATGGCATATATGCG
CTTGGTGAGTGGATAATTGGCGCAGGAGCACTTGGAGTATTTGTATACGGTGTGCTAAATAGGCTTTTGATTCCTTTTGG
ACTTCATCACGTTATAAACAGTCTTGTCTGGTTTGTTTTTGGTACATTTAAGACAGCAGCTGGCGAAGTTTTAACTGGCG
ATTTAAACAGGTTCTTTGCAGGAGACCCGACTGCAGGTATCTTTATGGCCGGATTTTATCCGATTATGATGTTTGGACTT
CCAGCAGCAGCACTGGCTATGTGGGCAGCAGCTAGGCCGAATCAAAGAAAAGTTGTGTCTGGTGTGTTAATAAGTGCAGC
TCTTACAGCCCTTTTAACGGGTATTACTGAACCCATCGAATTTGCATTTATGTTCTTGGCACCAGTACTTTATGTCATAC
ACGCCCTTTTGACAGGTTTGTCTCTCACTATAACTTATATTCTTGGAATAAAAGCTGGTTTTGGTTTTTCGGCTGGTCTT
ATTGACTATGTATTAAGCTATGGCATATCTACCAAACCGCTCTTACTTCTTTTGATAGGTATCATATACGGAGCAATATA
CTATGTAATTTTCTATTACGTAATAGTAAAATTCAATTTGCCAACTCCTGGCAGATTAGAAGAAGAAGCAACAGATCAGT
ATAAAGAATTGTCAAAATCAGAAATTGGAGGTATTGCTGCACAATACGTAGAGGTATTGGGTGGAGCAGAAAATATACAG
TCTTTGGAGGCTTGCATAACAAGATTGCGCTTGACTGTAAAAGACGATACAATAATAGACGATGATAAACTTAAAAAGTT
AGGGGCGACAGGCGTAATGAGAATGGGCAAAAATGCATTGCAAGTAATTGTTGGTACAAAAGCTGATTTGATTGCACAAG
AAATGAAAAAACACATGAAAAAAGCAGGAGGTAAAATTTAA

Upstream 100 bases:

>100_bases
GTGTTACTGCAAAAGCAGGCATGAGCCGGTTGAGGTGTTATTACCTTGCCGGCTCTTTTTGTATTAAATGCCTGAAAAAT
AAAAATAAGGAGGTTTTTGT

Downstream 100 bases:

>100_bases
GGTGAAAATGTTTTAGGCGGCCGTAAACTGGCCGCCATCAAAATTATTTTAAGAAGTTTGAAATATATGGGTATAATAAG
ATATTATTAAAATACAGAAA

Product: PTS system, N-acetylglucosamine-specific IIBC subunit

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKWLASVQKLGKALMLPVAVLPAAALLLRLGAPDVFNIPFIMQAGAAVFDNLPLIFAIGIAIGFAEGDGVAALAAAVGYF
VLTKGATTINKDINMGVLGGILMGIIAGYLYNKYHDIKLPDFLGFFGGKRFVPIVTAFAAIVLALVMGYVWPPIQNGIYA
LGEWIIGAGALGVFVYGVLNRLLIPFGLHHVINSLVWFVFGTFKTAAGEVLTGDLNRFFAGDPTAGIFMAGFYPIMMFGL
PAAALAMWAAARPNQRKVVSGVLISAALTALLTGITEPIEFAFMFLAPVLYVIHALLTGLSLTITYILGIKAGFGFSAGL
IDYVLSYGISTKPLLLLLIGIIYGAIYYVIFYYVIVKFNLPTPGRLEEEATDQYKELSKSEIGGIAAQYVEVLGGAENIQ
SLEACITRLRLTVKDDTIIDDDKLKKLGATGVMRMGKNALQVIVGTKADLIAQEMKKHMKKAGGKI

Sequences:

>Translated_466_residues
MKWLASVQKLGKALMLPVAVLPAAALLLRLGAPDVFNIPFIMQAGAAVFDNLPLIFAIGIAIGFAEGDGVAALAAAVGYF
VLTKGATTINKDINMGVLGGILMGIIAGYLYNKYHDIKLPDFLGFFGGKRFVPIVTAFAAIVLALVMGYVWPPIQNGIYA
LGEWIIGAGALGVFVYGVLNRLLIPFGLHHVINSLVWFVFGTFKTAAGEVLTGDLNRFFAGDPTAGIFMAGFYPIMMFGL
PAAALAMWAAARPNQRKVVSGVLISAALTALLTGITEPIEFAFMFLAPVLYVIHALLTGLSLTITYILGIKAGFGFSAGL
IDYVLSYGISTKPLLLLLIGIIYGAIYYVIFYYVIVKFNLPTPGRLEEEATDQYKELSKSEIGGIAAQYVEVLGGAENIQ
SLEACITRLRLTVKDDTIIDDDKLKKLGATGVMRMGKNALQVIVGTKADLIAQEMKKHMKKAGGKI
>Mature_466_residues
MKWLASVQKLGKALMLPVAVLPAAALLLRLGAPDVFNIPFIMQAGAAVFDNLPLIFAIGIAIGFAEGDGVAALAAAVGYF
VLTKGATTINKDINMGVLGGILMGIIAGYLYNKYHDIKLPDFLGFFGGKRFVPIVTAFAAIVLALVMGYVWPPIQNGIYA
LGEWIIGAGALGVFVYGVLNRLLIPFGLHHVINSLVWFVFGTFKTAAGEVLTGDLNRFFAGDPTAGIFMAGFYPIMMFGL
PAAALAMWAAARPNQRKVVSGVLISAALTALLTGITEPIEFAFMFLAPVLYVIHALLTGLSLTITYILGIKAGFGFSAGL
IDYVLSYGISTKPLLLLLIGIIYGAIYYVIFYYVIVKFNLPTPGRLEEEATDQYKELSKSEIGGIAAQYVEVLGGAENIQ
SLEACITRLRLTVKDDTIIDDDKLKKLGATGVMRMGKNALQVIVGTKADLIAQEMKKHMKKAGGKI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=464, Percent_Identity=56.6810344827586, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1787343, Length=478, Percent_Identity=46.0251046025105, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1787908, Length=512, Percent_Identity=38.4765625, Blast_Score=300, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49845; Mature: 49845

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWLASVQKLGKALMLPVAVLPAAALLLRLGAPDVFNIPFIMQAGAAVFDNLPLIFAIGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHCCHHHHHHHH
AIGFAEGDGVAALAAAVGYFVLTKGATTINKDINMGVLGGILMGIIAGYLYNKYHDIKLP
HHHCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
DFLGFFGGKRFVPIVTAFAAIVLALVMGYVWPPIQNGIYALGEWIIGAGALGVFVYGVLN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLIPFGLHHVINSLVWFVFGTFKTAAGEVLTGDLNRFFAGDPTAGIFMAGFYPIMMFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHC
PAAALAMWAAARPNQRKVVSGVLISAALTALLTGITEPIEFAFMFLAPVLYVIHALLTGL
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
SLTITYILGIKAGFGFSAGLIDYVLSYGISTKPLLLLLIGIIYGAIYYVIFYYVIVKFNL
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTPGRLEEEATDQYKELSKSEIGGIAAQYVEVLGGAENIQSLEACITRLRLTVKDDTIID
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCC
DDKLKKLGATGVMRMGKNALQVIVGTKADLIAQEMKKHMKKAGGKI
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]