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The map label for this gene is nodB

Identifier: 16519764

GI number: 16519764

Start: 435099

End: 435746

Strand: Direct

Name: nodB

Synonym: NGR_a03420

Alternate gene names: 16519764

Gene position: 435099-435746 (Clockwise)

Preceding gene: 16519765

Following gene: 16519763

Centisome position: 81.15

GC content: 61.11

Gene sequence:

>648_bases
ATGAAACAGCTTGACTATCTCCGGACAGTGCCCAGAAGCGGCACTGGGGCCCCGAGCGTCTACCTGACCTTTGACGACGG
TCCTAACCCGGTTTTTACCCCTGAGGTCCTCGATGTGCTGGCCGAACACCGTGTCCCGGCGACTTTCTTCGTCATCGGGG
CTTATGCTAAGGACCGGCCGCAACTCATCCGACGCATGGTTGCGCAAGGGCACGAGGTCGCCAACCATACAATGACCCAT
CCGGACCTGTCTGCCTGCGGACGTCGGGACGTGGAACGCCAAGTACTCGAGGCGAACAGGGCCATCAGGATGGCCTGCCC
CGAGGCTTCGGTGCGGCACATACGTGCGCCTTACGGAATTTGGAGCGATGACGTGCTGACAACATCGGCAAACGCTGGAC
TGGCGGCTGTGCACTGGTCTGTGGACCCGCGGGACTGGTCCCGCCCCGGTATCGACGCAATCGTCGATGCGGTACTCGCC
TCGGTCCGGCCCGGTTCAATCATTCTCCTGCACGATGGGTGCCCTCCCGACGAGTTGGCGAACACTGACGCCAGTTTTCG
CGACCAGACGGTGGCGGCGTTGTCTCGTCTCATCCCTGCCTTGCACGACCGCGGATTTATAATCCGCTCGCTTCCTCAGA
ATCATTAA

Upstream 100 bases:

>100_bases
CAACGCGCACTGAAGACGTACTCGTTGTGGTCTTTCCGATTGGACGTCCAATGAGCGAATGGCCATCCGGCACACTGATC
GAACGAAACGGTCCGGAACT

Downstream 100 bases:

>100_bases
ACGGACGGTTTTTCATGGATCTGCTTGGCACGACCGGCGCCGTAGCCATCTCCTTGTATGCAGCACTCTCGACGGCTTAC
AAAGGCATGCAAGCTATATA

Product: chitooligosaccharide deacetylase

Products: NA

Alternate protein names: Nodulation protein B

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKQLDYLRTVPRSGTGAPSVYLTFDDGPNPVFTPEVLDVLAEHRVPATFFVIGAYAKDRPQLIRRMVAQGHEVANHTMTH
PDLSACGRRDVERQVLEANRAIRMACPEASVRHIRAPYGIWSDDVLTTSANAGLAAVHWSVDPRDWSRPGIDAIVDAVLA
SVRPGSIILLHDGCPPDELANTDASFRDQTVAALSRLIPALHDRGFIIRSLPQNH

Sequences:

>Translated_215_residues
MKQLDYLRTVPRSGTGAPSVYLTFDDGPNPVFTPEVLDVLAEHRVPATFFVIGAYAKDRPQLIRRMVAQGHEVANHTMTH
PDLSACGRRDVERQVLEANRAIRMACPEASVRHIRAPYGIWSDDVLTTSANAGLAAVHWSVDPRDWSRPGIDAIVDAVLA
SVRPGSIILLHDGCPPDELANTDASFRDQTVAALSRLIPALHDRGFIIRSLPQNH
>Mature_215_residues
MKQLDYLRTVPRSGTGAPSVYLTFDDGPNPVFTPEVLDVLAEHRVPATFFVIGAYAKDRPQLIRRMVAQGHEVANHTMTH
PDLSACGRRDVERQVLEANRAIRMACPEASVRHIRAPYGIWSDDVLTTSANAGLAAVHWSVDPRDWSRPGIDAIVDAVLA
SVRPGSIILLHDGCPPDELANTDASFRDQTVAALSRLIPALHDRGFIIRSLPQNH

Specific function: Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NODB_RHISN (P50355)

Other databases:

- EMBL:   X73362
- EMBL:   U00090
- PIR:   S34304
- RefSeq:   NP_443884.1
- ProteinModelPortal:   P50355
- SMR:   P50355
- GeneID:   962186
- GenomeReviews:   U00090_GR
- KEGG:   rhi:NGR_a03420
- HOGENOM:   HBG751163
- ProtClustDB:   CLSK864446
- GO:   GO:0005737
- GO:   GO:0009877
- InterPro:   IPR011330
- InterPro:   IPR002509
- Gene3D:   G3DSA:3.20.20.370

Pfam domain/function: PF01522 Polysacc_deac_1; SSF88713 Glyco_hydro/deAcase_b/a-brl

EC number: NA

Molecular weight: Translated: 23625; Mature: 23625

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLDYLRTVPRSGTGAPSVYLTFDDGPNPVFTPEVLDVLAEHRVPATFFVIGAYAKDRP
CCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHH
QLIRRMVAQGHEVANHTMTHPDLSACGRRDVERQVLEANRAIRMACPEASVRHIRAPYGI
HHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCC
WSDDVLTTSANAGLAAVHWSVDPRDWSRPGIDAIVDAVLASVRPGSIILLHDGCPPDELA
CCCCEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHC
NTDASFRDQTVAALSRLIPALHDRGFIIRSLPQNH
CCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure
MKQLDYLRTVPRSGTGAPSVYLTFDDGPNPVFTPEVLDVLAEHRVPATFFVIGAYAKDRP
CCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHH
QLIRRMVAQGHEVANHTMTHPDLSACGRRDVERQVLEANRAIRMACPEASVRHIRAPYGI
HHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCC
WSDDVLTTSANAGLAAVHWSVDPRDWSRPGIDAIVDAVLASVRPGSIILLHDGCPPDELA
CCCCEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHC
NTDASFRDQTVAALSRLIPALHDRGFIIRSLPQNH
CCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984092; 9163424