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The map label for this gene is potH [H]

Identifier: 163845214

GI number: 163845214

Start: 1081474

End: 1082289

Strand: Reverse

Name: potH [H]

Synonym: BSUIS_B1101

Alternate gene names: 163845214

Gene position: 1082289-1081474 (Counterclockwise)

Preceding gene: 163845220

Following gene: 163845213

Centisome position: 77.26

GC content: 55.15

Gene sequence:

>816_bases
ATGTTTCTCCTTCTGCTCCCATTTTTCTCGCTATTGCTTCTGTTCTTCTTCTATCCGCTGGTGAATCTGCTCTCCTTGTC
AGTGACGGAACCGACGTTGTCACTTCATAACTATCAGCGCGTCTTTTCCAATCCCGTCTACCTGACTGTTTTGGGTCGCA
CGGTTCTAACGGCGGTGCTGGTGAGCGTCATTGCGTTGCTGATGGCCTATCCGGTGGCATGGTTGATGGCTCACTCCAGC
GGGCCAAAGCTGATTTTGATTACGGCGTGTATCCTCCTGCCGTTCTGGTCGTCGGTTCTCGTGCGTACCGCGGCCTGGGC
CGTGCTGTTGCAGCGCAATGGACTGGTCAATCAAGGGCTGATGCAGACCGGCCTCATCGATCAGCCGCTCAAACTGCTCT
ATACGCAAACAGCCGTTCTCATAGCCATGACACATGTGCTTTTGCCGTTCGTAGTGCTACCCATCTACGGTGCGATCCGC
AACGTGCCCAAGGATTATGGTCGTGCGGCTGCAATTCTCGGTGCAGGGCCTATGCGCACATTCTTTGAGGTCATTTTTCC
TCTGACGCTACCCGGCGTGATCGGCGGGATGATCCTGACATTCCTGACGGCCCTCGGTTACTTTATTACTCCCTCGCTTC
TTGGTTCGCCCAAGGAGATGATGATCGCCACCCTCATATCCCAGCAACTTCGCGAAACGCTGGACTGGCCCTTTGCCGCC
GCACTGGTGGGCGTCCTGACCGTGCTTGTGACTGTGGTGACATTGCTCTTCAGCCGCGTTTTCAAGTTCAACCGAATGAT
GGGAGGCGGCGTATGA

Upstream 100 bases:

>100_bases
GGCTTCTCGCCGGAAGCCGGCCTGCTCTATCGTCCGGCGGAGTAAGGCCATGTTGAAAATATTCTTCGAGCCGGATGGGC
GAAATATATCCAAAGGAGGG

Downstream 100 bases:

>100_bases
AAACGCGAAATCTGCTTCTGAACGCCTATGTCTATCTGGTGCTGTGCTTTCTGGTGGTGCCGATCCTGATCATCTTGCCG
ATGGCCTTCAGCGAAACCGG

Product: hypothetical protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MFLLLLPFFSLLLLFFFYPLVNLLSLSVTEPTLSLHNYQRVFSNPVYLTVLGRTVLTAVLVSVIALLMAYPVAWLMAHSS
GPKLILITACILLPFWSSVLVRTAAWAVLLQRNGLVNQGLMQTGLIDQPLKLLYTQTAVLIAMTHVLLPFVVLPIYGAIR
NVPKDYGRAAAILGAGPMRTFFEVIFPLTLPGVIGGMILTFLTALGYFITPSLLGSPKEMMIATLISQQLRETLDWPFAA
ALVGVLTVLVTVVTLLFSRVFKFNRMMGGGV

Sequences:

>Translated_271_residues
MFLLLLPFFSLLLLFFFYPLVNLLSLSVTEPTLSLHNYQRVFSNPVYLTVLGRTVLTAVLVSVIALLMAYPVAWLMAHSS
GPKLILITACILLPFWSSVLVRTAAWAVLLQRNGLVNQGLMQTGLIDQPLKLLYTQTAVLIAMTHVLLPFVVLPIYGAIR
NVPKDYGRAAAILGAGPMRTFFEVIFPLTLPGVIGGMILTFLTALGYFITPSLLGSPKEMMIATLISQQLRETLDWPFAA
ALVGVLTVLVTVVTLLFSRVFKFNRMMGGGV
>Mature_271_residues
MFLLLLPFFSLLLLFFFYPLVNLLSLSVTEPTLSLHNYQRVFSNPVYLTVLGRTVLTAVLVSVIALLMAYPVAWLMAHSS
GPKLILITACILLPFWSSVLVRTAAWAVLLQRNGLVNQGLMQTGLIDQPLKLLYTQTAVLIAMTHVLLPFVVLPIYGAIR
NVPKDYGRAAAILGAGPMRTFFEVIFPLTLPGVIGGMILTFLTALGYFITPSLLGSPKEMMIATLISQQLRETLDWPFAA
ALVGVLTVLVTVVTLLFSRVFKFNRMMGGGV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=228, Percent_Identity=37.719298245614, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1787369, Length=236, Percent_Identity=34.3220338983051, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1787713, Length=275, Percent_Identity=32, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788764, Length=217, Percent_Identity=26.2672811059908, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29760; Mature: 29760

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]