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The map label for this gene is cls [H]

Identifier: 163845173

GI number: 163845173

Start: 1033194

End: 1034654

Strand: Reverse

Name: cls [H]

Synonym: BSUIS_B1056

Alternate gene names: 163845173

Gene position: 1034654-1033194 (Counterclockwise)

Preceding gene: 163845175

Following gene: 163845169

Centisome position: 73.86

GC content: 57.97

Gene sequence:

>1461_bases
ATGCTTGATCTCGTTTCCCATTATTGGCCGCACATTCTGTTTGTCCTGTCGATCGTTCTCGGAGCGATTGCCGCCATTCA
TGCGACGATGACCAAGGAGGAAGTGCGCACGGCCATTGGCTGGGTCGGCGTGATCGTGCTGTCGCCCATCGTGGGGGCGG
TTGTCTATGCGATTGCGGGCGTCAACCGTATCCGGCGTTCGACACTGAGCTATCAGCGCGCCAATCTCAGCAATATCGCG
CTCTACCACCTCTCGCATTTCGACACGACGAACGACCGGGTGCGGGCCGCTTTCGGGCGGCAGTTCGGCGCGATGAAAAT
TCTGGGCGATGCGGTGAGCCTTTATGATTTTACCAGCGGCAACACTATTGAAATGCTGGAAACGGGTGATGAAGCCTATG
CCGCAATGCTTGGCGCCATAGGCCGGGCCGAGCGCAGCATTGTGCTGGAAACCTATATTTTCGACCATGACGCCATTGGT
AAAAAATTTGCCGATGCGCTTGGCGATGCGGTGCAGCGCGGCGTGGAAGTGCGGGTGCTGGTGGATGCGGTGGGCGCGCG
TTATTCCGTTCCAAGCATTGTGAAGCTTTTGAAAGAAAAGGGCGTCAAGGTCGCTGTGTTCAACGGCAATATCATCATCG
GCCTGCGTCTGCCCTATGCGAACCTGCGCACCCACCGCAAGATGCTGATTGTCGATGGTCAGACTGCCTTTACCGGCGGC
ATGAATATTCGCGCCGGGTTTGTGAGGGCTATCGCAGGCGATGCGGTTGCCTTCGATACACATTTCAAACTCGAAGGGCC
TGCCATTGCCGACCTCTTCCATATTGCCTCGGAAGACTGGCGTTTTGCGACCGGCGAGCTTTTGACGGGCGAAGCCTGGA
GCATCGCGCCACCGGAAAATCCACCCGGAACGGGAACGCTGGTGCGTGTCGTCGGGTCAGGGCCGGACAAGAACCTCGAA
GCGAACCATCGCATGATGATGGGGGCGTTTTCGATTGCCCAGCAGCATATTCTCATCATGACGCCCTATCTTTTGCCGGA
CCGCGAATTGATAAGTGCGCTGGTGACGGCGGCACGGCGCGGCGTATCGGTCGATATCGTGGTGCCGGGTGTCAACAATC
TGAAGCTGGTGGACCGCGCCATGCGCGCCCAGTTTGATCAGCTTTTGCGGGACGGCTGCCGCATCTGGCGTGCTGGCGGG
GCCTTCAATCACTCAAAGCTCATGACCATCGATGGCGCCTGGTCCTATGTCGGCTCGTCCAATATCGATCCGCGCTCGCT
GCGGCTCAATTTCGAGGTCGATCTTGAAATCCTCGACCGGGATGTGGCACGGCAGGTGGAAGAGCGTATCGGACGGGTGA
TCGAGAAAAGCCGTGAGGTCAACCTTGTGCGCCTGAAGAACCGCCCTTTTCTGGAGCGGCTGCTTGACAGGATCATCTGG
CTCGGCTCACCTTTTCTTTGA

Upstream 100 bases:

>100_bases
GCACAGGCTTGAACAGGCAGGATTTTCGCACTGTTCGTTTTGTGGGCAAAGCATTATATCTCTTTGCCGTTAAAGCCATT
TTTTGCTTAAAGTTTGCTGG

Downstream 100 bases:

>100_bases
TCGATCGCGCTATATTGTAGCGCTTTTGGATGCCTTGGAATTGTGGCTTTCCAACAGTTGCATGGCCGTTTTCAGGTCCA
GATGCGCCTTGATGGGCAGA

Product: hypothetical protein

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MLDLVSHYWPHILFVLSIVLGAIAAIHATMTKEEVRTAIGWVGVIVLSPIVGAVVYAIAGVNRIRRSTLSYQRANLSNIA
LYHLSHFDTTNDRVRAAFGRQFGAMKILGDAVSLYDFTSGNTIEMLETGDEAYAAMLGAIGRAERSIVLETYIFDHDAIG
KKFADALGDAVQRGVEVRVLVDAVGARYSVPSIVKLLKEKGVKVAVFNGNIIIGLRLPYANLRTHRKMLIVDGQTAFTGG
MNIRAGFVRAIAGDAVAFDTHFKLEGPAIADLFHIASEDWRFATGELLTGEAWSIAPPENPPGTGTLVRVVGSGPDKNLE
ANHRMMMGAFSIAQQHILIMTPYLLPDRELISALVTAARRGVSVDIVVPGVNNLKLVDRAMRAQFDQLLRDGCRIWRAGG
AFNHSKLMTIDGAWSYVGSSNIDPRSLRLNFEVDLEILDRDVARQVEERIGRVIEKSREVNLVRLKNRPFLERLLDRIIW
LGSPFL

Sequences:

>Translated_486_residues
MLDLVSHYWPHILFVLSIVLGAIAAIHATMTKEEVRTAIGWVGVIVLSPIVGAVVYAIAGVNRIRRSTLSYQRANLSNIA
LYHLSHFDTTNDRVRAAFGRQFGAMKILGDAVSLYDFTSGNTIEMLETGDEAYAAMLGAIGRAERSIVLETYIFDHDAIG
KKFADALGDAVQRGVEVRVLVDAVGARYSVPSIVKLLKEKGVKVAVFNGNIIIGLRLPYANLRTHRKMLIVDGQTAFTGG
MNIRAGFVRAIAGDAVAFDTHFKLEGPAIADLFHIASEDWRFATGELLTGEAWSIAPPENPPGTGTLVRVVGSGPDKNLE
ANHRMMMGAFSIAQQHILIMTPYLLPDRELISALVTAARRGVSVDIVVPGVNNLKLVDRAMRAQFDQLLRDGCRIWRAGG
AFNHSKLMTIDGAWSYVGSSNIDPRSLRLNFEVDLEILDRDVARQVEERIGRVIEKSREVNLVRLKNRPFLERLLDRIIW
LGSPFL
>Mature_486_residues
MLDLVSHYWPHILFVLSIVLGAIAAIHATMTKEEVRTAIGWVGVIVLSPIVGAVVYAIAGVNRIRRSTLSYQRANLSNIA
LYHLSHFDTTNDRVRAAFGRQFGAMKILGDAVSLYDFTSGNTIEMLETGDEAYAAMLGAIGRAERSIVLETYIFDHDAIG
KKFADALGDAVQRGVEVRVLVDAVGARYSVPSIVKLLKEKGVKVAVFNGNIIIGLRLPYANLRTHRKMLIVDGQTAFTGG
MNIRAGFVRAIAGDAVAFDTHFKLEGPAIADLFHIASEDWRFATGELLTGEAWSIAPPENPPGTGTLVRVVGSGPDKNLE
ANHRMMMGAFSIAQQHILIMTPYLLPDRELISALVTAARRGVSVDIVVPGVNNLKLVDRAMRAQFDQLLRDGCRIWRAGG
AFNHSKLMTIDGAWSYVGSSNIDPRSLRLNFEVDLEILDRDVARQVEERIGRVIEKSREVNLVRLKNRPFLERLLDRIIW
LGSPFL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=477, Percent_Identity=28.3018867924528, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787007, Length=344, Percent_Identity=29.3604651162791, Blast_Score=148, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 53549; Mature: 53549

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLVSHYWPHILFVLSIVLGAIAAIHATMTKEEVRTAIGWVGVIVLSPIVGAVVYAIAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRIRRSTLSYQRANLSNIALYHLSHFDTTNDRVRAAFGRQFGAMKILGDAVSLYDFTSG
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHCHHEEEEECCC
NTIEMLETGDEAYAAMLGAIGRAERSIVLETYIFDHDAIGKKFADALGDAVQRGVEVRVL
CEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEE
VDAVGARYSVPSIVKLLKEKGVKVAVFNGNIIIGLRLPYANLRTHRKMLIVDGQTAFTGG
EECCCCCCCHHHHHHHHHHCCCEEEEEECCEEEEEECCCHHHCCCCEEEEEECCEEEECC
MNIRAGFVRAIAGDAVAFDTHFKLEGPAIADLFHIASEDWRFATGELLTGEAWSIAPPEN
CCCHHHHHHHHCCCEEEEECEEEECCCHHHHHHHHCCCCCCEECCCEEECCCEECCCCCC
PPGTGTLVRVVGSGPDKNLEANHRMMMGAFSIAQQHILIMTPYLLPDRELISALVTAARR
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHC
GVSVDIVVPGVNNLKLVDRAMRAQFDQLLRDGCRIWRAGGAFNHSKLMTIDGAWSYVGSS
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCCCCC
NIDPRSLRLNFEVDLEILDRDVARQVEERIGRVIEKSREVNLVRLKNRPFLERLLDRIIW
CCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
LGSPFL
CCCCCC
>Mature Secondary Structure
MLDLVSHYWPHILFVLSIVLGAIAAIHATMTKEEVRTAIGWVGVIVLSPIVGAVVYAIAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRIRRSTLSYQRANLSNIALYHLSHFDTTNDRVRAAFGRQFGAMKILGDAVSLYDFTSG
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHCHHEEEEECCC
NTIEMLETGDEAYAAMLGAIGRAERSIVLETYIFDHDAIGKKFADALGDAVQRGVEVRVL
CEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEE
VDAVGARYSVPSIVKLLKEKGVKVAVFNGNIIIGLRLPYANLRTHRKMLIVDGQTAFTGG
EECCCCCCCHHHHHHHHHHCCCEEEEEECCEEEEEECCCHHHCCCCEEEEEECCEEEECC
MNIRAGFVRAIAGDAVAFDTHFKLEGPAIADLFHIASEDWRFATGELLTGEAWSIAPPEN
CCCHHHHHHHHCCCEEEEECEEEECCCHHHHHHHHCCCCCCEECCCEEECCCEECCCCCC
PPGTGTLVRVVGSGPDKNLEANHRMMMGAFSIAQQHILIMTPYLLPDRELISALVTAARR
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHC
GVSVDIVVPGVNNLKLVDRAMRAQFDQLLRDGCRIWRAGGAFNHSKLMTIDGAWSYVGSS
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCCCCC
NIDPRSLRLNFEVDLEILDRDVARQVEERIGRVIEKSREVNLVRLKNRPFLERLLDRIIW
CCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHH
LGSPFL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9443601 [H]