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The map label for this gene is oppA [H]

Identifier: 163844697

GI number: 163844697

Start: 520399

End: 521976

Strand: Reverse

Name: oppA [H]

Synonym: BSUIS_B0534

Alternate gene names: 163844697

Gene position: 521976-520399 (Counterclockwise)

Preceding gene: 163844698

Following gene: 163844696

Centisome position: 37.26

GC content: 54.31

Gene sequence:

>1578_bases
ATGTATCGCAAATTTCTACTGATGGGCGTGTGTCTTCTGGCGCTCGCGGGCGCAGCCTCGGCAGAGGCGGTGCTGAACCG
CGGCAATGACACCGATCCGGCCACGCTCGATCATCATCACACGTCCACCGTTGCCGAAGGCAATGTGTTGCGCGATCTTT
ATGATGGCCTGACCATTCAGGATGCTGACGGCAAGGCCATTCCCGGCGTAGCGAAATCCTGGGATATTTCCGAGGATGGC
ACGGTCTATACCTTCCATCTGCGCGACAACGCGAAATGGTCGAACGGCGATCCGCTTACCGCTGGCGACTTCCAGTTCAC
CTTCCGCCGTCTGATGGACCCGAAAACGGCTGACGGTTATGCCAGCATGTTGTTCATCATCAAGAATGCCGAAGATGTTG
CCGCTGGCAAGAAGCCTGTCGATCAGCTTGGCGTCGAGGCGGTTGACGATCATACGCTCAAGATCACGTTGAACTCCCCT
GCCCCTTATTTCCTTGAGCTTCTGACCCACCAGACAGGCTTCCCGCTGCATCAAAAAAGCGTTGAGGCCAATGGCGACAA
GTTCACCACGCCGGGCAAGATGGTTACGAACGGCGCCTATATGCTGGAAAGCTTCACGCCGAACGACAAGATCGTGATGA
AGAAGAATCCGTATTATTATGAAGCGGATCAGGTGAAGATCGATAAGGTCAACTGGATACCTTTCGAGGACCGCGCAAGC
TGCATGCGCCGCTTCGAGGCGAAAGAAGTACAAATCTGCTCCGATGTTCCGGCCGAGCAGATGGACTATGTGAAGAAGAA
CCTTTCCAAGGAATTCCGCATGGCGCCCTATCTCGGCGTCTATTACCTGCCGGTCAAGGGCAAGACTGCGGACAGCAAGC
TGAAAGATCCGCGCGTCCGCCAGGCGATTTCCATGGCTATCGACCGCGATTTCCTCGCCGATCAGGTCTGGCAGGGTACG
ATGATCCCAGGTTATTCCATGGTTCCGCCCGGCATCGCCAATTATGTGAAGGACGCGCCGAAGCTCGACTATTCCGACGA
CATGCTGGAGCGTGAGGACAAGGCCAAGGAATTGTTGAAGGAAGCAGGCGTGGAGCCGAATACGCTTTCCATCGAGCTTC
TCTACAATACGTCTGAAAACAACAAGAACACGATGGCAGCCATTGCAGACCAGCTTGGCAATATCGGCATCAAGGCCAGA
CTGAACGAAACCGAAGGCGCCAGCTATTTCAATTTCCTGCGCGAAGACGGCCCCTTCGATATCGTCCGCGCGAGCTGGAT
CGGCGATTATAATGATCCGCAGAACTTCCTGTACATCAGCCAGGGCAATGTCAGCTTCAACTATGCCAAGTGGAAGAACG
CCGATTATGATGCGCTGATGGCCAAGGCGGAAAAGACACAGGACCTGGGTGAACGAGCCAATATTCTGGCCGACGCCGCA
AAGATCCAGCTCAACGAATTGGGCGCTATTCCAATCCTTTATTATTCGTCGCTCGCGCTCGTTTCCAGCGATGTGGATGG
CTGGAACGACAACCTTATGAACAGCCACAAGACGCGTTGGCTTTCCCTTAAAAAATAA

Upstream 100 bases:

>100_bases
TATTTGAATGAATTCAATACGTTGATAAGTTTGCTTGCAAGTACCACGCTTATCCATAATATGCGGGCCATTTCAAAAAT
TTCAACGGAGGGGTTCTTCG

Downstream 100 bases:

>100_bases
AATTTTCAGCTTGGGCTGCGCTTATCAGCGCGGCCCTTGTCCTTCCGGGGGGCCCGAAGGGGCCGCGTTTTGACAAGCAT
TTTCCAAGAAAAGCATATGG

Product: hypothetical protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQDADGKAIPGVAKSWDISEDG
TVYTFHLRDNAKWSNGDPLTAGDFQFTFRRLMDPKTADGYASMLFIIKNAEDVAAGKKPVDQLGVEAVDDHTLKITLNSP
APYFLELLTHQTGFPLHQKSVEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVRQAISMAIDRDFLADQVWQGT
MIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLKEAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKAR
LNETEGASYFNFLREDGPFDIVRASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSHKTRWLSLKK

Sequences:

>Translated_525_residues
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQDADGKAIPGVAKSWDISEDG
TVYTFHLRDNAKWSNGDPLTAGDFQFTFRRLMDPKTADGYASMLFIIKNAEDVAAGKKPVDQLGVEAVDDHTLKITLNSP
APYFLELLTHQTGFPLHQKSVEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVRQAISMAIDRDFLADQVWQGT
MIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLKEAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKAR
LNETEGASYFNFLREDGPFDIVRASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSHKTRWLSLKK
>Mature_525_residues
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQDADGKAIPGVAKSWDISEDG
TVYTFHLRDNAKWSNGDPLTAGDFQFTFRRLMDPKTADGYASMLFIIKNAEDVAAGKKPVDQLGVEAVDDHTLKITLNSP
APYFLELLTHQTGFPLHQKSVEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVRQAISMAIDRDFLADQVWQGT
MIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLKEAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKAR
LNETEGASYFNFLREDGPFDIVRASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSHKTRWLSLKK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=517, Percent_Identity=36.9439071566731, Blast_Score=324, Evalue=7e-90,
Organism=Escherichia coli, GI87081878, Length=488, Percent_Identity=35.4508196721311, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1789397, Length=523, Percent_Identity=33.4608030592734, Blast_Score=275, Evalue=7e-75,
Organism=Escherichia coli, GI1787052, Length=521, Percent_Identity=26.4875239923225, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789966, Length=548, Percent_Identity=25.1824817518248, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787762, Length=531, Percent_Identity=25.9887005649718, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787551, Length=469, Percent_Identity=23.2409381663113, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1789887, Length=482, Percent_Identity=24.6887966804979, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58717; Mature: 58717

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQ
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHHCCCEEE
DADGKAIPGVAKSWDISEDGTVYTFHLRDNAKWSNGDPLTAGDFQFTFRRLMDPKTADGY
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
ASMLFIIKNAEDVAAGKKPVDQLGVEAVDDHTLKITLNSPAPYFLELLTHQTGFPLHQKS
EEEEEEEECCHHHHCCCCCHHHHCCEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCC
VEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CCCCCCEECCCCCEEECCEEEEEECCCCCEEEEECCCCEEECCCEEEEEEEECCCHHHHH
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVR
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHH
QAISMAIDRDFLADQVWQGTMIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKARLNETEGASYFNFLREDGPFD
HCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCEE
IVRASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
EEEEEECCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSHKTRWLSLKK
HEEHHHCCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEECC
>Mature Secondary Structure
MYRKFLLMGVCLLALAGAASAEAVLNRGNDTDPATLDHHHTSTVAEGNVLRDLYDGLTIQ
CCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHCCCCHHHHHHCCCEEE
DADGKAIPGVAKSWDISEDGTVYTFHLRDNAKWSNGDPLTAGDFQFTFRRLMDPKTADGY
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
ASMLFIIKNAEDVAAGKKPVDQLGVEAVDDHTLKITLNSPAPYFLELLTHQTGFPLHQKS
EEEEEEEECCHHHHCCCCCHHHHCCEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCC
VEANGDKFTTPGKMVTNGAYMLESFTPNDKIVMKKNPYYYEADQVKIDKVNWIPFEDRAS
CCCCCCEECCCCCEEECCEEEEEECCCCCEEEEECCCCEEECCCEEEEEEEECCCHHHHH
CMRRFEAKEVQICSDVPAEQMDYVKKNLSKEFRMAPYLGVYYLPVKGKTADSKLKDPRVR
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHH
QAISMAIDRDFLADQVWQGTMIPGYSMVPPGIANYVKDAPKLDYSDDMLEREDKAKELLK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EAGVEPNTLSIELLYNTSENNKNTMAAIADQLGNIGIKARLNETEGASYFNFLREDGPFD
HCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCEE
IVRASWIGDYNDPQNFLYISQGNVSFNYAKWKNADYDALMAKAEKTQDLGERANILADAA
EEEEEECCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KIQLNELGAIPILYYSSLALVSSDVDGWNDNLMNSHKTRWLSLKK
HEEHHHCCCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]