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The map label for this gene is pcaR [H]

Identifier: 163844407

GI number: 163844407

Start: 208786

End: 209640

Strand: Reverse

Name: pcaR [H]

Synonym: BSUIS_B0225

Alternate gene names: 163844407

Gene position: 209640-208786 (Counterclockwise)

Preceding gene: 163844410

Following gene: 163844406

Centisome position: 14.97

GC content: 58.95

Gene sequence:

>855_bases
ATGCGCAGCAGAACAGGCTCGGACGCTTCGAGACGGCAGGAACGCTCAGCCGAGATGCGGAAACCCACAATGCTTACCCC
CCTGGAGGCAGGAGTCGAGGAGGAGGATCGTCAATTCGTAACCGCGCTTGCCAGAGGGCTGGAGGTGCTTCGTTGCTTCA
CACCAACGGAAAACACCCTCGGCAATCAGGAAATCGCTCATAAGACAGGCCTTCCAAAACCCACGGTGTCACGGCTTACC
CATACGCTCGTGCGCCTTGGCTATCTGAGGCAGGATGCGCTTTCGGGGCTTTATCAGCTTGATATCGGGATATTGAGACT
TGGCTATGCCATGCTTTCAAACCTGATGATCCGAACGGTGGCATCCCCGCTGATGCAGGTGCTGGCAGATTATGCCAAAG
CCGCCGTGGCCATGGCCGCCCGCGACAGGTTGTCGATGGTTTATCTCGACGTGGTCCAGGGGGAAGGCAACATGACGATG
CGTCGCCAGATCGGCAGTACGCTGCCGCTGGCGGGAAGTTCTGTCGGCAGGGCCTGCCTTGCAGCCATGCCGGAGGACGA
ACGCACCTTCATTCTGAAGCATATTCGCGAGCGCGAACCGGAAAACTGGCCATCGATCCGCAAGGGACTGGATCGGGCAC
TCCGCGATTTCGAGGATTATGGCTATTGCCTGTCCATCGGCGAGTGGCATCGGGACGTCAATTCCGTGGCGGTGCCCCTC
GTGCACAAACAATATGGGGTGCTCGTCTTCAACTGCGGCGGACCAAGCTTCCAGCTACCGCGCGAGAAGCTGGAGGACGA
TATCGGCCCCCGCCTGATCGAGATGGTTCACAACATCAGCTCCGCTGTCCCGTAA

Upstream 100 bases:

>100_bases
TAATTTTTTAGGGGTAGGAATTATGTTTGCATTATGCTAGTTTCGCTATGCGAAATTTGATTTCGTATTATTGACAAGCA
AAACAAAATGGGGATTTTAA

Downstream 100 bases:

>100_bases
TCTGCCGGAAAGCGGAGCCTTGCAGAAAAGGCCGGCAACGGCCGCTAGGAGAATGCATGATCCGCGACCCGGAAATACTT
GAAATTCTGCGCGACACTGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRSRTGSDASRRQERSAEMRKPTMLTPLEAGVEEEDRQFVTALARGLEVLRCFTPTENTLGNQEIAHKTGLPKPTVSRLT
HTLVRLGYLRQDALSGLYQLDIGILRLGYAMLSNLMIRTVASPLMQVLADYAKAAVAMAARDRLSMVYLDVVQGEGNMTM
RRQIGSTLPLAGSSVGRACLAAMPEDERTFILKHIREREPENWPSIRKGLDRALRDFEDYGYCLSIGEWHRDVNSVAVPL
VHKQYGVLVFNCGGPSFQLPREKLEDDIGPRLIEMVHNISSAVP

Sequences:

>Translated_284_residues
MRSRTGSDASRRQERSAEMRKPTMLTPLEAGVEEEDRQFVTALARGLEVLRCFTPTENTLGNQEIAHKTGLPKPTVSRLT
HTLVRLGYLRQDALSGLYQLDIGILRLGYAMLSNLMIRTVASPLMQVLADYAKAAVAMAARDRLSMVYLDVVQGEGNMTM
RRQIGSTLPLAGSSVGRACLAAMPEDERTFILKHIREREPENWPSIRKGLDRALRDFEDYGYCLSIGEWHRDVNSVAVPL
VHKQYGVLVFNCGGPSFQLPREKLEDDIGPRLIEMVHNISSAVP
>Mature_284_residues
MRSRTGSDASRRQERSAEMRKPTMLTPLEAGVEEEDRQFVTALARGLEVLRCFTPTENTLGNQEIAHKTGLPKPTVSRLT
HTLVRLGYLRQDALSGLYQLDIGILRLGYAMLSNLMIRTVASPLMQVLADYAKAAVAMAARDRLSMVYLDVVQGEGNMTM
RRQIGSTLPLAGSSVGRACLAAMPEDERTFILKHIREREPENWPSIRKGLDRALRDFEDYGYCLSIGEWHRDVNSVAVPL
VHKQYGVLVFNCGGPSFQLPREKLEDDIGPRLIEMVHNISSAVP

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1788131, Length=216, Percent_Identity=26.3888888888889, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082359, Length=175, Percent_Identity=28, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786715, Length=249, Percent_Identity=24.4979919678715, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786468, Length=212, Percent_Identity=25, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31737; Mature: 31737

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRTGSDASRRQERSAEMRKPTMLTPLEAGVEEEDRQFVTALARGLEVLRCFTPTENTL
CCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GNQEIAHKTGLPKPTVSRLTHTLVRLGYLRQDALSGLYQLDIGILRLGYAMLSNLMIRTV
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASPLMQVLADYAKAAVAMAARDRLSMVYLDVVQGEGNMTMRRQIGSTLPLAGSSVGRACL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCHHHHHHH
AAMPEDERTFILKHIREREPENWPSIRKGLDRALRDFEDYGYCLSIGEWHRDVNSVAVPL
HHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHH
VHKQYGVLVFNCGGPSFQLPREKLEDDIGPRLIEMVHNISSAVP
HHHCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSRTGSDASRRQERSAEMRKPTMLTPLEAGVEEEDRQFVTALARGLEVLRCFTPTENTL
CCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
GNQEIAHKTGLPKPTVSRLTHTLVRLGYLRQDALSGLYQLDIGILRLGYAMLSNLMIRTV
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASPLMQVLADYAKAAVAMAARDRLSMVYLDVVQGEGNMTMRRQIGSTLPLAGSSVGRACL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCHHHHHHH
AAMPEDERTFILKHIREREPENWPSIRKGLDRALRDFEDYGYCLSIGEWHRDVNSVAVPL
HHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHH
VHKQYGVLVFNCGGPSFQLPREKLEDDIGPRLIEMVHNISSAVP
HHHCCCEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]