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The map label for this gene is ybiS [H]

Identifier: 163844373

GI number: 163844373

Start: 169384

End: 170118

Strand: Reverse

Name: ybiS [H]

Synonym: BSUIS_B0187

Alternate gene names: 163844373

Gene position: 170118-169384 (Counterclockwise)

Preceding gene: 163844379

Following gene: 163844372

Centisome position: 12.14

GC content: 60.68

Gene sequence:

>735_bases
ATGAAACCACGTCATCTTCTTTTGATCGGATCACTGACGGCAATCGCCGTCTCCATCGTCAGCACCGGCATGGCCTTTGC
CGATGACCGTTATTCCGATCTGCCGCCTGTGCGCGTGAGCCAGGCGATTTCCGCACCATGGGTCGCGCAGTTGCAGGACG
CACCGGCCCGGCCACGCATCGCACGGCAGGAAACAAGAAAAACCAAAACGCACTATAACAAGGTTTCTTACCAGCGCCCG
GCGACAAACCCGGCAACCACGGCACGCAAGAAGCAGTTTGACCCGATCTATCTGCCGCAGATCGTTTCCTACTCGGGCAA
GGAAAAGCCCGGCACCATCGTCATCGATACGGCCAGCCGTTTTCTCTATCTGGTACAGGCCGACGGCAAGGCACGCCGCT
ACGGTGTCGGCGTCGGCAAGCAGGGGTTCAGCTGGAAAGGCACACAGCGCATCAGCCGCAAGGCCGAATGGCCCACATGG
ACGCCGCCCAGTGAAATGATTGCCCGCGAGCGCAAGAAGGGCCGTTTCCTGCCCGCACGCATGGATGGCGGGATCAACAA
TCCGCTCGGCGCCCGCGCGCTTTATCTGGGTTCAACCCTCTACCGCATCCATGGCACCAACCAGCCGTGGACCATCGGCA
AGGCCATGTCCTCGGGCTGCATTCGTATGCGTAACGAGGATGTGACCGACCTTTACGAACGCACACCGGTGGGAACCCGC
GTGGTGGTCCGCTGA

Upstream 100 bases:

>100_bases
CATGAACTTCGGGGGCGCTCTCCTGACAGCAGATGTCAGGAGATGCGACGGGCAACCGCGCCAGCCTGGTGAGCGGCGCA
AAGATAGACGGATCAAAACC

Downstream 100 bases:

>100_bases
GGGATTCACAAGCGCAAACAGCGGTTTTTATGGGGGTCGCCTTACGGGAAGGCGACCCAGTTATTTTTCACAAGTTTGAA
ATATCCGAATTTCCTGCAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKPRHLLLIGSLTAIAVSIVSTGMAFADDRYSDLPPVRVSQAISAPWVAQLQDAPARPRIARQETRKTKTHYNKVSYQRP
ATNPATTARKKQFDPIYLPQIVSYSGKEKPGTIVIDTASRFLYLVQADGKARRYGVGVGKQGFSWKGTQRISRKAEWPTW
TPPSEMIARERKKGRFLPARMDGGINNPLGARALYLGSTLYRIHGTNQPWTIGKAMSSGCIRMRNEDVTDLYERTPVGTR
VVVR

Sequences:

>Translated_244_residues
MKPRHLLLIGSLTAIAVSIVSTGMAFADDRYSDLPPVRVSQAISAPWVAQLQDAPARPRIARQETRKTKTHYNKVSYQRP
ATNPATTARKKQFDPIYLPQIVSYSGKEKPGTIVIDTASRFLYLVQADGKARRYGVGVGKQGFSWKGTQRISRKAEWPTW
TPPSEMIARERKKGRFLPARMDGGINNPLGARALYLGSTLYRIHGTNQPWTIGKAMSSGCIRMRNEDVTDLYERTPVGTR
VVVR
>Mature_244_residues
MKPRHLLLIGSLTAIAVSIVSTGMAFADDRYSDLPPVRVSQAISAPWVAQLQDAPARPRIARQETRKTKTHYNKVSYQRP
ATNPATTARKKQFDPIYLPQIVSYSGKEKPGTIVIDTASRFLYLVQADGKARRYGVGVGKQGFSWKGTQRISRKAEWPTW
TPPSEMIARERKKGRFLPARMDGGINNPLGARALYLGSTLYRIHGTNQPWTIGKAMSSGCIRMRNEDVTDLYERTPVGTR
VVVR

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=134, Percent_Identity=43.2835820895522, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788299, Length=136, Percent_Identity=40.4411764705882, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787356, Length=135, Percent_Identity=35.5555555555556, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1787968, Length=151, Percent_Identity=35.0993377483444, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 27286; Mature: 27286

Theoretical pI: Translated: 11.18; Mature: 11.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRHLLLIGSLTAIAVSIVSTGMAFADDRYSDLPPVRVSQAISAPWVAQLQDAPARPRI
CCCCEEEEEEHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCH
ARQETRKTKTHYNKVSYQRPATNPATTARKKQFDPIYLPQIVSYSGKEKPGTIVIDTASR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEECCCE
FLYLVQADGKARRYGVGVGKQGFSWKGTQRISRKAEWPTWTPPSEMIARERKKGRFLPAR
EEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHH
MDGGINNPLGARALYLGSTLYRIHGTNQPWTIGKAMSSGCIRMRNEDVTDLYERTPVGTR
CCCCCCCCCCHHHHHHCCEEEEEECCCCCEEHHHHHHCCEEEECCCCHHHHHHHCCCCEE
VVVR
EEEC
>Mature Secondary Structure
MKPRHLLLIGSLTAIAVSIVSTGMAFADDRYSDLPPVRVSQAISAPWVAQLQDAPARPRI
CCCCEEEEEEHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCH
ARQETRKTKTHYNKVSYQRPATNPATTARKKQFDPIYLPQIVSYSGKEKPGTIVIDTASR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEEECCCE
FLYLVQADGKARRYGVGVGKQGFSWKGTQRISRKAEWPTWTPPSEMIARERKKGRFLPAR
EEEEEECCCCCEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHH
MDGGINNPLGARALYLGSTLYRIHGTNQPWTIGKAMSSGCIRMRNEDVTDLYERTPVGTR
CCCCCCCCCCHHHHHHCCEEEEEECCCCCEEHHHHHHCCEEEECCCCHHHHHHHCCCCEE
VVVR
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]