BacMap

Definition	Brucella suis ATCC 23445 chromosome II, complete genome.
Accession	NC_010167
Length	1,400,844

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The map label for this gene is yqhE [C]

Identifier: 163844372

GI number: 163844372

Start: 168281

End: 169108

Strand: Reverse

Name: yqhE [C]

Synonym: BSUIS_B0186

Alternate gene names: 163844372

Gene position: 169108-168281 (Counterclockwise)

Preceding gene: 163844373

Following gene: 163844368

Centisome position: 12.07

GC content: 55.31

Gene sequence:

>828_bases
ATGACCGTACCGACGGTTAAACTGAACGACGGCAATCATATTCCGCAGCTCGGTTACGGTGTCTGGCAGATTAGCAATGA
TGAAGCGGTGAGCGCAGTCAGCGAGGCGCTGAAAGCCGGTTATCGACACATCGACACCGCCACCATTTACGGCAATGAAA
AAGGCGTCGGCAAGGCGATCAACGGTTCGGGCATCGCGCGCGCAGACATCTTCCTGACCACCAAGCTCTGGAACAGCGAT
CAGGGCTATGAATCCACGCTCAAGGCTTTCGACACCAGCCTGAAGAAGCTGGGCACCGACTATGTGGATCTCTATCTGAT
CCACTGGCCCATGCCATCGAAAGACCTGTTCATGGAAACATGGCGCGCCTTCATCAAACTGAAGGAAGAAGGCCGCGTCA
AATCCATCGGTGTATCGAATTTCCGCACTGCCGATCTTGAGCGCCTCATCAAGGAAAGCGGCGTTACGCCGGTCCTGAAC
CAGATCGAGTTGCACCCCCAGTTCCAGCAGGATGAGCTGCGCCTGTTCCATGGCAAGCACGACATCGCAACGGAAGCCTG
GAGCCCGCTCGGCCAGGGCAAACTTCTGGAAGACCCGACGCTGAAATCGATTGCCGAAAAGCACGCAAAGTCGGTGGCAC
AGATCATTTTGCGCTGGCACATCGAGACCGGGAATATCGTGATCCCGAAATCGATCACACCTGCCCGCATCAAGGAAAAT
TTCGATATTTTCGACTTCACGCTGAACGGCACCGACCACGATGCCATCACCAAGCTTGACATGGCGGATGGCCGCATCGG
GCCGAACCCGAGTGTTTTTTCAGCATAA

Upstream 100 bases:

>100_bases
CAAAGTCCGAAGAATATTCGTCAAAGAGCTGCCGCGAGGGATAATGTGGTGGCCAGCGCCCCGGAAAACGGCGCAAAAGA
TAAGTGGGAAAGGATATATC

Downstream 100 bases:

>100_bases
ATGGAGCATGTCCCCAAAAGCGGAAGCCGGTTTTGGGGATTAGAGCGCGTTTCGATCTGATTGAATCAGATCGAAACGCG
CTCTAAACATTACAAAAAAC

Product: hypothetical protein

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEKGVGKAINGSGIARADIFLTTKLWNSD
QGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMETWRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLN
QIELHPQFQQDELRLFHGKHDIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKEN
FDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA

Sequences:

>Translated_275_residues
MTVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEKGVGKAINGSGIARADIFLTTKLWNSD
QGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMETWRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLN
QIELHPQFQQDELRLFHGKHDIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKEN
FDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA
>Mature_274_residues
TVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEKGVGKAINGSGIARADIFLTTKLWNSDQ
GYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMETWRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLNQ
IELHPQFQQDELRLFHGKHDIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKENF
DIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=38.7755102040816, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI223468663, Length=289, Percent_Identity=38.4083044982699, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI5453543, Length=294, Percent_Identity=38.7755102040816, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=39.4927536231884, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI310109920, Length=294, Percent_Identity=38.7755102040816, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI45446745, Length=294, Percent_Identity=38.7755102040816, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI4503285, Length=294, Percent_Identity=38.7755102040816, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI93277124, Length=294, Percent_Identity=38.4353741496599, Blast_Score=191, Evalue=4e-49,
Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=34.4709897610922, Blast_Score=191, Evalue=4e-49,
Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=34.4709897610922, Blast_Score=191, Evalue=4e-49,
Organism=Homo sapiens, GI24497585, Length=294, Percent_Identity=37.7551020408163, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI5174695, Length=294, Percent_Identity=37.7551020408163, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=38.0228136882129, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=37.5451263537906, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI4502049, Length=279, Percent_Identity=38.3512544802867, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=33.9350180505415, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI310109926, Length=126, Percent_Identity=43.6507936507937, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI207028673, Length=126, Percent_Identity=43.6507936507937, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI310109924, Length=148, Percent_Identity=38.5135135135135, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI87082198, Length=278, Percent_Identity=51.0791366906475, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1786400, Length=257, Percent_Identity=37.3540856031128, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=30.8550185873606, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI48994888, Length=237, Percent_Identity=28.6919831223629, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=39.2592592592593, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17561298, Length=270, Percent_Identity=35.9259259259259, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17537077, Length=291, Percent_Identity=37.1134020618557, Blast_Score=184, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17537075, Length=301, Percent_Identity=35.8803986710963, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17564128, Length=298, Percent_Identity=35.9060402684564, Blast_Score=180, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=37.5, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=34.7169811320755, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17566692, Length=283, Percent_Identity=36.7491166077738, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17550248, Length=288, Percent_Identity=36.8055555555556, Blast_Score=171, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17537079, Length=300, Percent_Identity=32.6666666666667, Blast_Score=160, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17561300, Length=254, Percent_Identity=34.6456692913386, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17562292, Length=254, Percent_Identity=33.0708661417323, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324694, Length=301, Percent_Identity=42.1926910299003, Blast_Score=217, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6320576, Length=290, Percent_Identity=41.0344827586207, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322556, Length=252, Percent_Identity=36.5079365079365, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319625, Length=279, Percent_Identity=38.3512544802867, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320079, Length=280, Percent_Identity=39.2857142857143, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321896, Length=303, Percent_Identity=33.6633663366337, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24657054, Length=278, Percent_Identity=41.726618705036, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI21356425, Length=282, Percent_Identity=40.0709219858156, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24662789, Length=301, Percent_Identity=36.5448504983389, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24663317, Length=280, Percent_Identity=39.2857142857143, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI20129731, Length=275, Percent_Identity=37.8181818181818, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24644950, Length=296, Percent_Identity=38.1756756756757, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI221378297, Length=316, Percent_Identity=36.0759493670886, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI45553081, Length=275, Percent_Identity=38.9090909090909, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24662781, Length=300, Percent_Identity=39.6666666666667, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24662785, Length=297, Percent_Identity=39.3939393939394, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI281366140, Length=239, Percent_Identity=38.4937238493724, Blast_Score=151, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 30640; Mature: 30509

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEKGVGKAI
CCCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEE
NGSGIARADIFLTTKLWNSDQGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMET
CCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHH
WRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLNQIELHPQFQQDELRLFHGKH
HHHHHEECCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHCCCC
DIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKEN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCHHHHHCC
FDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA
CCEEEEEECCCCCHHEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEKGVGKAI
CCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEE
NGSGIARADIFLTTKLWNSDQGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMET
CCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHH
WRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLNQIELHPQFQQDELRLFHGKH
HHHHHEECCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHCCCC
DIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKEN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCHHHHHCC
FDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA
CCEEEEEECCCCCHHEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA