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The map label for this gene is ponB

Identifier: 16273611

GI number: 16273611

Start: 1796174

End: 1798519

Strand: Direct

Name: ponB

Synonym: HI1725

Alternate gene names: 16273611

Gene position: 1796174-1798519 (Clockwise)

Preceding gene: 30995474

Following gene: 30995475

Centisome position: 98.14

GC content: 40.49

Gene sequence:

>2346_bases
ATGACTTCTCAACACTCCGCTAAAAAATCATCGAAAAACACGCCAAAAAATAACCGCACTTTTAAAGGTTTTTTACTTAA
ATTTAGCTTCACTGCACTTGTCTTAACGATTTTTTACGGCGGTTATCTCGATTGGCAAATTCGTTCTAAAATGGACGGTC
AAATTTGGCATTTGCCTGCGGAAGTTTATAGTCGATTAGAAAGTGTGAAAATTGCCGATAATCTCGCTTTTGATGAAGTC
ATTCAAATTTTATTAGATAACGAATATCGCCAAACGACCATGGTTGCCGCCCCTGGCGATTTCAAATTAGAAGATGATAG
CATTGTAGTGCTTCGCCGCGCTTTTCCTTTCCCCGATAAAGCAGAGCCTCAGCGAGTTTTGCGCTTGCGTTTTAGCCATA
ATAAGTTATCTCGCATTGAAGATTTAGTGACGGTCAAAACTGTTGATGAATTTCGTCTTGCACCAAAGTTGATTGCGATG
TTGCAATCAGACAACGAAGATCGCCTTGCTATTCCTTTACAAAATTATCCCCGTTTATTAATTGATACATTAATTTTAAC
GGAAGATCGCCGCTTTTATGAACATAATGGCATTAATCCAGTGGGGATTTTACGTGCATTAATTGCGAATATTCGTGCTG
GTCAAACGGTACAGGGCGGAAGTACGCTCACTCAACAATTAGTCAAAAATTTATTTTTATCGCGCGAACGCACCATTACA
CGCAAAGCCAACGAAGCCTTGATGTCATTGGTTTTAGATTGGCGTTACGATAAAAACCGAATTCTCGAAACCTATCTCAA
TGAAATTTATCTCGGGCAAAATGGCGATACGCAAATTCACGGTTTTGAATTAGCCAGCCAATTTTATTTTGGTCGTTCGA
TTCGTGAAATTAGCTTAGATCAAATTGCCCTTTTAGTGGGGATGGTCAAAGGCCCTTCACTTTATAATCCGTGGCGTAAT
CCACAAAACGCACTCGAACGTCGCAACATTGTTTTAAGGCTAATGCTTGAACATAAAATGATCGGCGATGAACTTTATCA
ATTATTAAGTCAGCGACCTTTAGGTGTGCAAAAGAAAGGGCAAATCTCGCGTAAATACCCTGCATTTATTCAAACTTTAC
AAGCAGATTTACGCCGTAAGCTGGGCGAACACAAAATATCGAGCTTACTTGGCGCACGTATTTTTTCCACAATGGATTTA
AAACAACAAGCTCAAGCAGAAAATGCAGTGGGCAATACCGTTTCGCAATTACAACTGAAAATGAAAAATCCCCATTTAGA
GGGAGCGATGATTATAACGGATTATCGCACAGGCGAAATTCGTGCAGTAGTTGGCGGTTTACAAACGCAATATGCAGGCT
TTAATCGTGCATTAATGGCAAAACGTCAGATTGGTTCTTTAGTAAAACCTTCTATTTATTTGACCGCACTTTCTAACCCA
GAACAGTTCCGTTTGAATACCCCAATTAATAACCAACCAATAACAATTAATGTCAAAGGTAGCCCGCCGTGGCAGCCACG
CAACTATGACAAAAAATATAGTGATTCTGTAATGTTAATGGATGCCTTAGCCCGTTCTTTAAATATTCCCACTGTAAATA
TTGGTATGAAAGTTGGCTTGAGCAAAGTCATTGACACGCAAAAAGCGATGGGCTGGGACAACGTGGAAATTCCTAAAGTG
CCTGCGATGTTGCTCGGTTCTTATACCATCTCGCCTTATGATGTCACAAAACTTTATCAAACCCTTGCCAATCAAGGTGG
GAGAATTGCGCTTACAACCGTCGATAGTATTGCAGATCGTCAAGGTAACCTAATTTTTCAACACGATAAAAGTGCAAAAC
AAGTGGTGCCACAAGAAGCGGCATTCCAAACCTTATTTGCAATGCAACAAACTGTTGAACGTGGTACAGCTCGCAGTTTG
CAAAAGGATTACGCTGATTTACATTTAGCAGGCAAAACAGGCACAACCAATGAATCACGCGATACGTGGTTTGTGGGAAT
TGACGGAAAAAATATCAGCACCGTCTGGCTTGGTCGCGATGATAATGGCGAAACAAAACTGACTGGTGCTTCTGGCGCAT
TGCAAATTTATAAAGATTATTTAAACCGTACAAATATTGAAAAATTAGCAATTACTCCCCCTACAACAGTGAAATGGGTG
GGGATAAATCAATACGGTGACTGGGATTGTGAGAGTTATCGAACAATTCCTATTTGGCTAAATAATGGTCAGAATTTCTG
TGGCGAAACATCATCTCCCTCTCTCACGCCAACAACTGAAACAGAAACACCACCACAAGAAAGCCTGTGGGACGTATTGG
ATAATCCCAATCCACCCGCTCAATAA

Upstream 100 bases:

>100_bases
AGATTTCCATTATTATGACGAAGAAAGCCTTGCTTTTTGCGGTACGCAAGATTTTATTCATTTTCTCCAATCTTATGTTG
ATTTTATTGCTTAACTCACT

Downstream 100 bases:

>100_bases
TTAAGCCGTAGGATTTACTAAATTTCCAATAATAAACATTTTCCCCCTATACTTCCCCTAACATTTCAGAGGATTTTTAT
TTCTTTTTAAAAAATAGCAA

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase

Number of amino acids: Translated: 781; Mature: 780

Protein sequence:

>781_residues
MTSQHSAKKSSKNTPKNNRTFKGFLLKFSFTALVLTIFYGGYLDWQIRSKMDGQIWHLPAEVYSRLESVKIADNLAFDEV
IQILLDNEYRQTTMVAAPGDFKLEDDSIVVLRRAFPFPDKAEPQRVLRLRFSHNKLSRIEDLVTVKTVDEFRLAPKLIAM
LQSDNEDRLAIPLQNYPRLLIDTLILTEDRRFYEHNGINPVGILRALIANIRAGQTVQGGSTLTQQLVKNLFLSRERTIT
RKANEALMSLVLDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASQFYFGRSIREISLDQIALLVGMVKGPSLYNPWRN
PQNALERRNIVLRLMLEHKMIGDELYQLLSQRPLGVQKKGQISRKYPAFIQTLQADLRRKLGEHKISSLLGARIFSTMDL
KQQAQAENAVGNTVSQLQLKMKNPHLEGAMIITDYRTGEIRAVVGGLQTQYAGFNRALMAKRQIGSLVKPSIYLTALSNP
EQFRLNTPINNQPITINVKGSPPWQPRNYDKKYSDSVMLMDALARSLNIPTVNIGMKVGLSKVIDTQKAMGWDNVEIPKV
PAMLLGSYTISPYDVTKLYQTLANQGGRIALTTVDSIADRQGNLIFQHDKSAKQVVPQEAAFQTLFAMQQTVERGTARSL
QKDYADLHLAGKTGTTNESRDTWFVGIDGKNISTVWLGRDDNGETKLTGASGALQIYKDYLNRTNIEKLAITPPTTVKWV
GINQYGDWDCESYRTIPIWLNNGQNFCGETSSPSLTPTTETETPPQESLWDVLDNPNPPAQ

Sequences:

>Translated_781_residues
MTSQHSAKKSSKNTPKNNRTFKGFLLKFSFTALVLTIFYGGYLDWQIRSKMDGQIWHLPAEVYSRLESVKIADNLAFDEV
IQILLDNEYRQTTMVAAPGDFKLEDDSIVVLRRAFPFPDKAEPQRVLRLRFSHNKLSRIEDLVTVKTVDEFRLAPKLIAM
LQSDNEDRLAIPLQNYPRLLIDTLILTEDRRFYEHNGINPVGILRALIANIRAGQTVQGGSTLTQQLVKNLFLSRERTIT
RKANEALMSLVLDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASQFYFGRSIREISLDQIALLVGMVKGPSLYNPWRN
PQNALERRNIVLRLMLEHKMIGDELYQLLSQRPLGVQKKGQISRKYPAFIQTLQADLRRKLGEHKISSLLGARIFSTMDL
KQQAQAENAVGNTVSQLQLKMKNPHLEGAMIITDYRTGEIRAVVGGLQTQYAGFNRALMAKRQIGSLVKPSIYLTALSNP
EQFRLNTPINNQPITINVKGSPPWQPRNYDKKYSDSVMLMDALARSLNIPTVNIGMKVGLSKVIDTQKAMGWDNVEIPKV
PAMLLGSYTISPYDVTKLYQTLANQGGRIALTTVDSIADRQGNLIFQHDKSAKQVVPQEAAFQTLFAMQQTVERGTARSL
QKDYADLHLAGKTGTTNESRDTWFVGIDGKNISTVWLGRDDNGETKLTGASGALQIYKDYLNRTNIEKLAITPPTTVKWV
GINQYGDWDCESYRTIPIWLNNGQNFCGETSSPSLTPTTETETPPQESLWDVLDNPNPPAQ
>Mature_780_residues
TSQHSAKKSSKNTPKNNRTFKGFLLKFSFTALVLTIFYGGYLDWQIRSKMDGQIWHLPAEVYSRLESVKIADNLAFDEVI
QILLDNEYRQTTMVAAPGDFKLEDDSIVVLRRAFPFPDKAEPQRVLRLRFSHNKLSRIEDLVTVKTVDEFRLAPKLIAML
QSDNEDRLAIPLQNYPRLLIDTLILTEDRRFYEHNGINPVGILRALIANIRAGQTVQGGSTLTQQLVKNLFLSRERTITR
KANEALMSLVLDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASQFYFGRSIREISLDQIALLVGMVKGPSLYNPWRNP
QNALERRNIVLRLMLEHKMIGDELYQLLSQRPLGVQKKGQISRKYPAFIQTLQADLRRKLGEHKISSLLGARIFSTMDLK
QQAQAENAVGNTVSQLQLKMKNPHLEGAMIITDYRTGEIRAVVGGLQTQYAGFNRALMAKRQIGSLVKPSIYLTALSNPE
QFRLNTPINNQPITINVKGSPPWQPRNYDKKYSDSVMLMDALARSLNIPTVNIGMKVGLSKVIDTQKAMGWDNVEIPKVP
AMLLGSYTISPYDVTKLYQTLANQGGRIALTTVDSIADRQGNLIFQHDKSAKQVVPQEAAFQTLFAMQQTVERGTARSLQ
KDYADLHLAGKTGTTNESRDTWFVGIDGKNISTVWLGRDDNGETKLTGASGALQIYKDYLNRTNIEKLAITPPTTVKWVG
INQYGDWDCESYRTIPIWLNNGQNFCGETSSPSLTPTTETETPPQESLWDVLDNPNPPAQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786343, Length=731, Percent_Identity=47.8796169630643, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=285, Percent_Identity=33.6842105263158, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788867, Length=592, Percent_Identity=25.8445945945946, Blast_Score=138, Evalue=2e-33,
Organism=Escherichia coli, GI1789601, Length=154, Percent_Identity=33.1168831168831, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPB_HAEIN (P45345)

Other databases:

- EMBL:   L42023
- PIR:   D64138
- RefSeq:   NP_439866.1
- ProteinModelPortal:   P45345
- SMR:   P45345
- GeneID:   950545
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1725
- NMPDR:   fig|71421.1.peg.1637
- TIGR:   HI_1725
- HOGENOM:   HBG672432
- OMA:   PAEVYSR
- ProtClustDB:   CLSK870113
- BioCyc:   HINF71421:HI_1725-MONOMER
- BRENDA:   2.4.1.129
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460
- PIRSF:   PIRSF002799
- TIGRFAMs:   TIGR02071

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: =2.4.1.129

Molecular weight: Translated: 88258; Mature: 88127

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: ACT_SITE 188-188 ACT_SITE 466-466

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQHSAKKSSKNTPKNNRTFKGFLLKFSFTALVLTIFYGGYLDWQIRSKMDGQIWHLPA
CCCCCCCHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEHH
EVYSRLESVKIADNLAFDEVIQILLDNEYRQTTMVAAPGDFKLEDDSIVVLRRAFPFPDK
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCEECCCCEEEEEECCCCCCC
AEPQRVLRLRFSHNKLSRIEDLVTVKTVDEFRLAPKLIAMLQSDNEDRLAIPLQNYPRLL
CCHHHHHHHHHCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHH
IDTLILTEDRRFYEHNGINPVGILRALIANIRAGQTVQGGSTLTQQLVKNLFLSRERTIT
HHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHH
RKANEALMSLVLDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASQFYFGRSIREISLD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCEEEHHHHHHHHHHCCCHHHHHHH
QIALLVGMVKGPSLYNPWRNPQNALERRNIVLRLMLEHKMIGDELYQLLSQRPLGVQKKG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QISRKYPAFIQTLQADLRRKLGEHKISSLLGARIFSTMDLKQQAQAENAVGNTVSQLQLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEE
MKNPHLEGAMIITDYRTGEIRAVVGGLQTQYAGFNRALMAKRQIGSLVKPSIYLTALSNP
ECCCCCCCEEEEEECCCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCC
EQFRLNTPINNQPITINVKGSPPWQPRNYDKKYSDSVMLMDALARSLNIPTVNIGMKVGL
CEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHH
SKVIDTQKAMGWDNVEIPKVPAMLLGSYTISPYDVTKLYQTLANQGGRIALTTVDSIADR
HHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHCC
QGNLIFQHDKSAKQVVPQEAAFQTLFAMQQTVERGTARSLQKDYADLHLAGKTGTTNESR
CCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
DTWFVGIDGKNISTVWLGRDDNGETKLTGASGALQIYKDYLNRTNIEKLAITPPTTVKWV
CEEEEEECCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEECCCCEEEEE
GINQYGDWDCESYRTIPIWLNNGQNFCGETSSPSLTPTTETETPPQESLWDVLDNPNPPA
ECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
Q
C
>Mature Secondary Structure 
TSQHSAKKSSKNTPKNNRTFKGFLLKFSFTALVLTIFYGGYLDWQIRSKMDGQIWHLPA
CCCCCCHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEHH
EVYSRLESVKIADNLAFDEVIQILLDNEYRQTTMVAAPGDFKLEDDSIVVLRRAFPFPDK
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCEECCCCEEEEEECCCCCCC
AEPQRVLRLRFSHNKLSRIEDLVTVKTVDEFRLAPKLIAMLQSDNEDRLAIPLQNYPRLL
CCHHHHHHHHHCCCHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCHHH
IDTLILTEDRRFYEHNGINPVGILRALIANIRAGQTVQGGSTLTQQLVKNLFLSRERTIT
HHHHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHH
RKANEALMSLVLDWRYDKNRILETYLNEIYLGQNGDTQIHGFELASQFYFGRSIREISLD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCEEEHHHHHHHHHHCCCHHHHHHH
QIALLVGMVKGPSLYNPWRNPQNALERRNIVLRLMLEHKMIGDELYQLLSQRPLGVQKKG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QISRKYPAFIQTLQADLRRKLGEHKISSLLGARIFSTMDLKQQAQAENAVGNTVSQLQLK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEE
MKNPHLEGAMIITDYRTGEIRAVVGGLQTQYAGFNRALMAKRQIGSLVKPSIYLTALSNP
ECCCCCCCEEEEEECCCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCC
EQFRLNTPINNQPITINVKGSPPWQPRNYDKKYSDSVMLMDALARSLNIPTVNIGMKVGL
CEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHH
SKVIDTQKAMGWDNVEIPKVPAMLLGSYTISPYDVTKLYQTLANQGGRIALTTVDSIADR
HHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHCC
QGNLIFQHDKSAKQVVPQEAAFQTLFAMQQTVERGTARSLQKDYADLHLAGKTGTTNESR
CCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
DTWFVGIDGKNISTVWLGRDDNGETKLTGASGALQIYKDYLNRTNIEKLAITPPTTVKWV
CEEEEEECCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEECCCCEEEEE
GINQYGDWDCESYRTIPIWLNNGQNFCGETSSPSLTPTTETETPPQESLWDVLDNPNPPA
ECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800