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The map label for this gene is ptsH

Identifier: 16273600

GI number: 16273600

Start: 1785712

End: 1785969

Strand: Reverse

Name: ptsH

Synonym: HI1713

Alternate gene names: 16273600

Gene position: 1785969-1785712 (Counterclockwise)

Preceding gene: 16273601

Following gene: 16273599

Centisome position: 97.59

GC content: 39.53

Gene sequence:

>258_bases
ATGTATTCAAAAGATGTGGAAATTATTGCGTCAAACGGTTTACACACGCGCCCTGCTGCACAATTTGTGAAAGAAGCGAA
AGCATTTTCTTCTGAAATTACAGTAACCTCTGGGGGGAAAAGTGCAAGTGCAAAGAGCTTATTTAAGTTGCAAACATTAG
CTTTAACGCAGGGAACAATACTTACCATTTCTGCTGATGGAGAAGACGAGCAACAGGCAGTTGAACATTTAGTCGCGTTA
ATTCCAACTTTAGAATAA

Upstream 100 bases:

>100_bases
GCTTTTTAGTGTTTATTCTTGATTATTTTGCTTTGAAAAAGGATACTAGGGCAATTTTCTGGTTTTATATTTTTAATTAT
TGATTAGTCGAGGTAGCATT

Downstream 100 bases:

>100_bases
TTCTTACTTAGCCATAAAATTGTGAAATTTTATGGCTATTGTTTATTTATTACTTTGTTAATTGTTTCGGAAGGTATCTA
TGATTTCTGGCATTCTCGCA

Product: phosphocarrier protein HPr

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein

Number of amino acids: Translated: 85; Mature: 85

Protein sequence:

>85_residues
MYSKDVEIIASNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLALTQGTILTISADGEDEQQAVEHLVAL
IPTLE

Sequences:

>Translated_85_residues
MYSKDVEIIASNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLALTQGTILTISADGEDEQQAVEHLVAL
IPTLE
>Mature_85_residues
MYSKDVEIIASNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLALTQGTILTISADGEDEQQAVEHLVAL
IPTLE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain

Homologues:

Organism=Escherichia coli, GI1788755, Length=85, Percent_Identity=77.6470588235294, Blast_Score=135, Evalue=6e-34,

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PTHP_HAEIN (P43921)

Other databases:

- EMBL:   L42023
- PIR:   I64137
- RefSeq:   NP_439855.1
- ProteinModelPortal:   P43921
- SMR:   P43921
- GeneID:   950527
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1713
- TIGR:   HI_1713
- HOGENOM:   HBG653254
- OMA:   DEQQAVE
- ProtClustDB:   CLSK870289
- BioCyc:   HINF71421:HI_1713-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.30.1340.10
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; SSF55594 HPr_protein

EC number: NA

Molecular weight: Translated: 9004; Mature: 9004

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 15-15

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSKDVEIIASNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLALTQGTI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHEECCCEE
LTISADGEDEQQAVEHLVALIPTLE
EEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYSKDVEIIASNGLHTRPAAQFVKEAKAFSSEITVTSGGKSASAKSLFKLQTLALTQGTI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHEECCCEE
LTISADGEDEQQAVEHLVALIPTLE
EEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800