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The map label for this gene is ptsI

Identifier: 16273599

GI number: 16273599

Start: 1783905

End: 1785632

Strand: Reverse

Name: ptsI

Synonym: HI1712

Alternate gene names: 16273599

Gene position: 1785632-1783905 (Counterclockwise)

Preceding gene: 16273600

Following gene: 16273598

Centisome position: 97.57

GC content: 37.15

Gene sequence:

>1728_bases
ATGATTTCTGGCATTCTCGCATCTCCAGGTATTGCCTTTGGTAAGGCACTTGTTCTTAAAGAAGAAAAGATTGTTCTTGA
TACACAAAAAATTACTGATGATCAAATTGACGCAGAAGTCGCTCGATTTTATGAAGGGCGTAACGCGGCGGTAGAACAAC
TTAATTCAATTAGAGAACGTGCATTAATTTCTTTAGGCGAAGAAAAAGCGGCAATCTTTGAAGGTCACTTGATGATTCTT
GAGGATGAAGAATTAGAAGAAGAAATTCTTGATTATCTTCGTTCAAATAAAGTGAATGCAGGCGTTGCGGCGAGTAAAAT
TTTAGATCAACAAGTGACAATGTTATCTGAAATTGATGATGAATATTTGAAAGAACGTGCAGGCGATATTCGGGATATTG
CTAACCGTTTAGTTAAAAATATTTTGGGAATGTACATTGTTGATCTTGGTGATATTCAGGAAGAATCTATTCTTGTTGCT
TATGATCTGACTCCCTCAGAAACAGCTCAACTTAATCTTGAAAAAGTATTAGGGGTTGTCACTGATATTGGTGGTAGAAC
CTCACATACTTCTATTATGGCTCGTTCTTTAGAATTGCCAGCGATTGTCGGCACAAATAAAGTGACTAAATTAGTCAATA
CGGGCGATTATTTAATTTTAGATGCTATCAATAACCAAGTTTATATTAATCCAACAGCATCTCAGATTGATGAATTAAAA
GCACTTGAAGCGAAAATTTCTGAGGAAAAAGCAGAGTTAGCTAAATTAAAAGATCTTCCTGCAATCACATTAGATGGACA
TAAGGTTGATGTGGTTGCGAATATCGGGACGATTCGTGATTGTGACGGCGCAGAACGTAATGGAGCGGAAGGGATTGGGT
TATACCGAACTGAATTCTTATTTATGGATCGTGAACAGCTTCCCACAGAAGAAGAACAATTTATTGCCTATAAACAAGTG
GTAGAAGCAATGAACGGACGTTTAACAGTGATTCGTACTATGGATATTGGTGGCGATAAAGAGCTTTCATATTTAGATTT
ACCAAAAGAAATGAATCCATTCTTGGGATGGCGTGCGATTCGCATTGCATTAGATCGTCGTGAAATTTTAAATGCACAAT
TACGTGCGGTGTTACGTGCTTCAGCTTTTGGTAAATTGGCGGTGATGTTCCCGATGATTATTTCTGTAGAAGAAATTCGG
GAATTAAAAGCGGTTATTGAAACATTAAAAGCGGAATTACGTGAAGAGGGGAGACTGTTTGATAATAATATCCAAGTTGG
TGTAATGGTCGAAACCCCCTCTGCCGCGGTAAATGCAAAATTTTTAGCAAAAGAAGTAGATTTCTTTAGTATCGGTACTA
ACGATTTAACTCAATATACTTTGGCTGTAGATCGCGGTAATGAGTTTATTTCCCATCTTTATAATCCGATGCACCCCTCT
GTGCTTGGTTTAATTAAACAAGTGATTGATGCCTCTCACGCAGAAGGAAAATGGACGGGAATGTGTGGGGAATTAGCAGG
GGATGAACGTGCGACTTTATTATTGCTTGGTATGGGATTAGATGAATTTAGTATGAGTGCGATTTCTGTACCTCGAATTA
AAAAATTAATCCGTAATGTAAATTTTCAAGATGCAAAAGTGCTTGCTGATACTGCATTACAAAAACCGACCGCTGCAGAA
ATTGATCAATTAATTGAAGAATTTTTGCTGGAAAATTCATTAAATTAA

Upstream 100 bases:

>100_bases
TTAATTCCAACTTTAGAATAATTCTTACTTAGCCATAAAATTGTGAAATTTTATGGCTATTGTTTATTTATTACTTTGTT
AATTGTTTCGGAAGGTATCT

Downstream 100 bases:

>100_bases
ACAAAATTATTTGATTTTACGTTAAAATAGCAACCATCTTGAATGGATAGGAGATTAAAATGGGCTTATTTGACAAGTTA
TTTGGTTCAAAAGAAAACAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRNAAVEQLNSIRERALISLGEEKAAIFEGHLMIL
EDEELEEEILDYLRSNKVNAGVAASKILDQQVTMLSEIDDEYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVA
YDLTPSETAQLNLEKVLGVVTDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAINNQVYINPTASQIDELK
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFLFMDREQLPTEEEQFIAYKQV
VEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAIRIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAVIETLKAELREEGRLFDNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNVNFQDAKVLADTALQKPTAAE
IDQLIEEFLLENSLN

Sequences:

>Translated_575_residues
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRNAAVEQLNSIRERALISLGEEKAAIFEGHLMIL
EDEELEEEILDYLRSNKVNAGVAASKILDQQVTMLSEIDDEYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVA
YDLTPSETAQLNLEKVLGVVTDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAINNQVYINPTASQIDELK
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFLFMDREQLPTEEEQFIAYKQV
VEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAIRIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAVIETLKAELREEGRLFDNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNVNFQDAKVLADTALQKPTAAE
IDQLIEEFLLENSLN
>Mature_575_residues
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRNAAVEQLNSIRERALISLGEEKAAIFEGHLMIL
EDEELEEEILDYLRSNKVNAGVAASKILDQQVTMLSEIDDEYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVA
YDLTPSETAQLNLEKVLGVVTDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAINNQVYINPTASQIDELK
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFLFMDREQLPTEEEQFIAYKQV
VEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAIRIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAVIETLKAELREEGRLFDNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNVNFQDAKVLADTALQKPTAAE
IDQLIEEFLLENSLN

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1788756, Length=574, Percent_Identity=70.383275261324, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=526, Percent_Identity=39.3536121673004, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI48994992, Length=521, Percent_Identity=37.0441458733205, Blast_Score=324, Evalue=7e-90,
Organism=Escherichia coli, GI1789193, Length=579, Percent_Identity=32.6424870466321, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1787994, Length=430, Percent_Identity=27.906976744186, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI226510935, Length=208, Percent_Identity=25.4807692307692, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PT1_HAEIN (P43922)

Other databases:

- EMBL:   L42023
- PIR:   H64137
- RefSeq:   NP_439854.1
- ProteinModelPortal:   P43922
- SMR:   P43922
- GeneID:   950872
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1712
- TIGR:   HI_1712
- HOGENOM:   HBG456539
- OMA:   MDREQLP
- ProtClustDB:   PRK11177
- BioCyc:   HINF71421:HI_1712-MONOMER
- BRENDA:   2.7.3.9
- GO:   GO:0005737
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:1.10.274.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01417

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; SSF47831 PEP-utilisers_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.3.9

Molecular weight: Translated: 63692; Mature: 63692

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 189-189 ACT_SITE 502-502 BINDING 296-296 BINDING 332-332 BINDING 431-431 BINDING 452-452 BINDING 453-453 BINDING 454-454 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRNAAVEQLNSIRER
CCCCCCCCCCHHHCCEEEEECCEEEEEHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
ALISLGEEKAAIFEGHLMILEDEELEEEILDYLRSNKVNAGVAASKILDQQVTMLSEIDD
HHHHCCCCHHEEEECCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
EYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVAYDLTPSETAQLNLEKVLGVV
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAINNQVYINPTASQIDELK
HHCCCCCCHHHHHHHHCCCCEEECCCHHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHH
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFL
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEHEEE
FMDREQLPTEEEQFIAYKQVVEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAI
EECHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHCCHHHCCEEE
RIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIRELKAVIETLKAELREEGRLF
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
DNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
CCCEEEEEEEECCCCHHHHHHHHHHCCEEECCCCCCEEEEEEEECCHHHHHHHCCCCCHH
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNV
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHCC
NFQDAKVLADTALQKPTAAEIDQLIEEFLLENSLN
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRNAAVEQLNSIRER
CCCCCCCCCCHHHCCEEEEECCEEEEEHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
ALISLGEEKAAIFEGHLMILEDEELEEEILDYLRSNKVNAGVAASKILDQQVTMLSEIDD
HHHHCCCCHHEEEECCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCH
EYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVAYDLTPSETAQLNLEKVLGVV
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAINNQVYINPTASQIDELK
HHCCCCCCHHHHHHHHCCCCEEECCCHHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHH
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFL
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEHEEE
FMDREQLPTEEEQFIAYKQVVEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAI
EECHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHCCHHHCCEEE
RIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIRELKAVIETLKAELREEGRLF
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
DNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
CCCEEEEEEEECCCCHHHHHHHHHHCCEEECCCCCCEEEEEEEECCHHHHHHHCCCCCHH
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNV
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHCC
NFQDAKVLADTALQKPTAAEIDQLIEEFLLENSLN
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800