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The map label for this gene is nth

Identifier: 16273576

GI number: 16273576

Start: 1759288

End: 1759923

Strand: Direct

Name: nth

Synonym: HI1689

Alternate gene names: 16273576

Gene position: 1759288-1759923 (Clockwise)

Preceding gene: 16273575

Following gene: 16273577

Centisome position: 96.13

GC content: 38.21

Gene sequence:

>636_bases
ATGAACAAAACAAAAAGAATCGAAATACTCACAAGATTACGAGAGCAAAACCCCCACCCGACCACAGAATTACAGTATAA
TTCGCCCTTTGAATTATTAATTGCGGTCATTCTATCGGCGCAAGCAACCGATAAAGGCGTGAATAAAGCAACAGAAAAAC
TATTCCCCGTGGCAAACACGCCTCAAGCGATTTTAGATTTAGGATTAGATGGTTTAAAAAGCTATATTAAAACTATCGGG
CTTTTTAATAGCAAAGCAGAAAATATTATCAAAACTTGCCGAGATTTAATTGAAAAGCACAACGGTGAAGTGCCCGAAAA
TCGCGAAGCCTTAGAAGCATTAGCGGGTGTGGGTAGAAAAACAGCCAATGTTGTGCTAAATACGGCATTTGGTCATCCAA
CTATTGCGGTAGATACCCATATTTTCCGTGTATGCAATCGCACTAATTTTGCAGCGGGTAAAGATGTGGTAAAAGTGGAA
GAAAAGCTGCTTAAAGTCGTACCTAATGAATTTAAAGTGGATGTTCATCATTGGCTAATTTTACACGGACGCTATACTTG
TATCGCGCGTAAACCTCGTTGTGGATCTTGCATTATTGAAGATCTTTGTGAATATAAAGAAAAAGTTGAATTTTAA

Upstream 100 bases:

>100_bases
CCTGCTTGCGGGGGAAAGTGTCACGAAGCGACGGAAGGGGGCTTACAGTCTTCTAAACAGATAATTCCAATAAATTAAAT
AGGCTAATTTCTACAAAAAT

Downstream 100 bases:

>100_bases
TTTTGCGGTAAAACAACCTAATTATTTACCGCACTTTTATTAAAAATAGGATAAAAAATGACAACAAACAATAAACAGCG
ACAAACTTGGTCGAGCCGCC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKSYIKTIG
LFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVE
EKLLKVVPNEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVEF

Sequences:

>Translated_211_residues
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKSYIKTIG
LFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVE
EKLLKVVPNEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVEF
>Mature_211_residues
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKSYIKTIG
LFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVE
EKLLKVVPNEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVEF

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1787920, Length=211, Percent_Identity=83.4123222748815, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=30.2857142857143, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=190, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END3_HAEIN (P44319)

Other databases:

- EMBL:   L42023
- PIR:   G64136
- RefSeq:   NP_439831.1
- ProteinModelPortal:   P44319
- SMR:   P44319
- GeneID:   950507
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1689
- NMPDR:   fig|71421.1.peg.1602
- TIGR:   HI_1689
- HOGENOM:   HBG464473
- OMA:   FGEPTIA
- ProtClustDB:   CLSK870076
- BioCyc:   HINF71421:HI_1689-MONOMER
- BRENDA:   4.2.99.18
- GO:   GO:0005622
- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478
- SMART:   SM00525
- SMART:   SM00278
- TIGRFAMs:   TIGR01083

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD; SSF48150 DNA_glycsylse

EC number: =4.2.99.18

Molecular weight: Translated: 23716; Mature: 23716

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00764 ENDONUCLEASE_III_1; PS01155 ENDONUCLEASE_III_2

Important sites: ACT_SITE 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANT
CCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQAILDLGLDGLKSYIKTIGLFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRK
HHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCH
TANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVEEKLLKVVPNEFKVDVHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHEEHHHHEEE
LHGRYTCIARKPRCGSCIIEDLCEYKEKVEF
EECCEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKTKRIEILTRLREQNPHPTTELQYNSPFELLIAVILSAQATDKGVNKATEKLFPVANT
CCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQAILDLGLDGLKSYIKTIGLFNSKAENIIKTCRDLIEKHNGEVPENREALEALAGVGRK
HHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCH
TANVVLNTAFGHPTIAVDTHIFRVCNRTNFAAGKDVVKVEEKLLKVVPNEFKVDVHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHEEHHHHEEE
LHGRYTCIARKPRCGSCIIEDLCEYKEKVEF
EECCEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800