Click here to switch to the map view.

The map label for this gene is sucB

Identifier: 16273548

GI number: 16273548

Start: 1728171

End: 1729400

Strand: Reverse

Name: sucB

Synonym: HI1661

Alternate gene names: 16273548

Gene position: 1729400-1728171 (Counterclockwise)

Preceding gene: 30995467

Following gene: 16273541

Centisome position: 94.5

GC content: 40.41

Gene sequence:

>1230_bases
ATGGCAATCGAAATTCTTGTTCCAGACCTACCTGAATCAGTTGCAGATGCAACTGTTGCCACTTGGCATAAAAAACTGGG
CGATACTGTGAAACGTGATGAGGTTATTGTGGAAATTGAAACGGATAAAGTCGTGCTAGAAGTTCCCGCACTTTCTGATG
GTGTACTGGCTGAAGTTGTTCAAGCTGAGGGCGAAACAGTGGTGAGTAAACAGTTGCTAGGCAAAATTTCTACGGCTCAA
GAAGGAGATGTCAGTTCAGCGACGTTAAAAGCAACGAATGAACCCACACCATCTGATCGTCAAAATGCAGCGATTGAAAA
TAGTCATAATCATAATGCGGATCAGAGCCCTGTAATTCGTCGTTTATTAGCAGAACATGATTTACAAGCAGATCAAATTC
AAGGTTCGGGTGTGGGTGGTCGTTTAACACGTGAAGATATTGAACGTGAAATTGCAAAACGACAAGCACAGCAAGTGAAA
CAAGAAGCTGCCACTGAGCAAAATACAATTAGTACGGTGGCTTATAGCGCACGTTCTGAAAAACGTGTGCCAATGACCCG
TTTGCGTAAACGTATTGCTGAACGTTTACTTGAAGCCAAAAATAGTACGGCGATGCTTACCACTTTCAATGAAGTGGATA
TGCAGCCGATAATGACTCTGCGTAAAACCTACGGCGAAAAATTTGAAAAACAACATTCAGTACGTTTAGGATTTATGTCT
TTTTATATTAAAGCGGTTGTGGAAGCATTAAAACGTTATCCTGAAGTTAATGCTTCTATTGATGGGGATGATGTTGTTTA
CCATAACTATTTTGATATTAGTATTGCAGTTTCAACACCACGTGGATTAGTCACACCAGTGCTTCGTGATTGCGATAAGC
TCAGTATGGCAGAGATCGAAAAACAAATTAAAGCATTAGCTGAAAAAGGTCGTGATGGCAAATTAACAGTAGAAGATCTT
ACTGGCGGCAATTTTACTATTACAAATGGTGGCGTGTTCGGTTCTCTTATGTCCACTCCAATTATCAATCCACCACAAAG
TGCGATTTTAGGAATGCACGCTATTAAAGAACGCCCGATTGCGCTTAATGGTCAAGTTGTGATTCGTCCGATGATGTATC
TTGCCTTATCTTATGATCATCGCCTAATTGACGGCCGTGAATCTGTTGGTTTCTTGGTAACGATTAAAGAATTATTGGAA
GATCCAACAAGATTGTTATTAGAAATCTAA

Upstream 100 bases:

>100_bases
TGCGGTTAATTTAATAAAGGTTTTTATCTTTGCTTTCCTCTGTAAATAGGGGAGCCAAGATAATTAAGATATAGTAAAAA
AGAAGAAAAGGAAAAATAAA

Downstream 100 bases:

>100_bases
GAAATAAAAGGCTTTCTGTGTTAGAAAGCCTTTTAAAATGAACAAAAAATTAACCGCACTTTAAATATTTAAAAAGAATA
TTCCAAAGTGCGGTTAGTTT

Product: 2-oxoglutarate dehydrogenase E2 component dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MAIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGETVVSKQLLGKISTAQ
EGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPVIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVK
QEAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMS
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDL
TGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLE
DPTRLLLEI

Sequences:

>Translated_409_residues
MAIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGETVVSKQLLGKISTAQ
EGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPVIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVK
QEAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMS
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDL
TGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLE
DPTRLLLEI
>Mature_408_residues
AIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGETVVSKQLLGKISTAQE
GDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPVIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQ
EAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMSF
YIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDLT
GGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLED
PTRLLLEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=58.5470085470086, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=27.9379157427938, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=27.9379157427938, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=28.0459770114943, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=26.3279445727483, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=36.3095238095238, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=73.7226277372263, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=29.1764705882353, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=402, Percent_Identity=41.5422885572139, Blast_Score=310, Evalue=8e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=30.3854875283447, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=23.6533957845433, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=28.6644951140065, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=42.4691358024691, Blast_Score=328, Evalue=7e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=25.9911894273128, Blast_Score=152, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=60, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=28.3018867924528, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24582497, Length=296, Percent_Identity=31.0810810810811, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI20129315, Length=296, Percent_Identity=31.0810810810811, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODO2_HAEIN (P45302)

Other databases:

- EMBL:   L42023
- PIR:   D64135
- RefSeq:   NP_439803.1
- ProteinModelPortal:   P45302
- GeneID:   950492
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1661
- NMPDR:   fig|71421.1.peg.1575
- TIGR:   HI_1661
- HOGENOM:   HBG630916
- OMA:   MLTTYNE
- ProtClustDB:   CLSK892422
- BioCyc:   HINF71421:HI_1661-MONOMER
- BRENDA:   2.3.1.61
- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10
- TIGRFAMs:   TIGR01347

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.61

Molecular weight: Translated: 45163; Mature: 45032

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 380-380 ACT_SITE 384-384

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVV
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHH
QAEGETVVSKQLLGKISTAQEGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPVIR
HHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQEAATEQNTISTVAYSARSE
HHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMS
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCHHHHHHH
KQIKALAEKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPI
HHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
ALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLEI
EECCCCHHHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCHHHHHHCC
>Mature Secondary Structure 
AIEILVPDLPESVADATVATWHKKLGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVV
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHH
QAEGETVVSKQLLGKISTAQEGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPVIR
HHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQEAATEQNTISTVAYSARSE
HHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHSVRLGFMS
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCHHHHHHH
KQIKALAEKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPI
HHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
ALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLEI
EECCCCHHHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800