Click here to switch to the map view.

The map label for this gene is rsmI

Identifier: 16273541

GI number: 16273541

Start: 1720014

End: 1720865

Strand: Reverse

Name: rsmI

Synonym: HI1654

Alternate gene names: 16273541

Gene position: 1720865-1720014 (Counterclockwise)

Preceding gene: 16273548

Following gene: 16273540

Centisome position: 94.03

GC content: 40.38

Gene sequence:

>852_bases
ATGACTGATTTAACCGGAATTTTATACATTGTTGCCACGCCCATTGGCAATTTACAAGATATTACCCAACGTGCTTTAGA
GACTTTTGCTCAAGTGGATTTAATTGCAGCAGAAGATACTCGCCATAGTGGACTTTTATTGAGCCATTACGGCATTAAGA
AGCCATTTTTTGCTTTGCACGATCATAACGAACAAGAAAAAGCGCATATTTTGGTGGAAAAGCTCAAGCAGGGGAGTAAT
ATTGCCTTGATTTCTGATGCGGGGACGCCATTAATCAGTGACCCTGGTTTTCATTTAGTACGCCAATGCCGTGAAGCTGG
CATTCGAGTTGTGCCTTTGCCAGGAGCTTGTGCGGCAATTACCGCTCTTTGTGCATCGGGGATTGCTTCTGATAGATTTT
GTTTTGAAGGCTTTTTACCTGCGAAAAGTAAAGCACGCAAAGATAAATTAGAAAATATCGCAGAAGAAGACCGCACTTTG
ATTTTTTATGAATCCACTCACCGTATTTTAGATACACTAGAAGATATGCAAGCGGTGCTAGGGGAAGAACGATACATTGT
GTTAGCCCGTGAAATGACTAAAACTTGGGAAACGATTACGGGGAATACGATTAAAAATTTACGCGAATGGCTTTTAGAAG
ATCCCAATCGTACAAAAGGCGAGATGGTTTTGATTGTGGAAGGCAAACCAAAGTCTGACAATAACGATGAAATTTCGCCG
CAAGCGGTAAAGGCACTTGAGTTAATTGCAGAAGAATTGCCACTTAAAAAAGCAGCAGCTATAGTTGCTGAGTTGTATGG
TTATAAGAAGAATGCTTTGTATCAATTTGGATTAGCGCATTTGGAAAAATAA

Upstream 100 bases:

>100_bases
AAACGTTCGCCTTGTAATAGAATAGACATCTTTGCTCCATCATAAACAGACAATAGTCGTAGCAGATCTTACTTATCTCA
TAACTTAAAAGCAAACAAAA

Downstream 100 bases:

>100_bases
CCTCAAAATCAACCGCACTTTATTAATCCGATTTTAATCTGTGCTGTTGTATTAAACGGATACAAGCACTCACAAGATAT
TTACTTTAGACTGGAAAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA 2'-O-ribose C1402 methyltransferase; rRNA (cytidine-2'-O-)-methyltransferase RsmI

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTDLTGILYIVATPIGNLQDITQRALETFAQVDLIAAEDTRHSGLLLSHYGIKKPFFALHDHNEQEKAHILVEKLKQGSN
IALISDAGTPLISDPGFHLVRQCREAGIRVVPLPGACAAITALCASGIASDRFCFEGFLPAKSKARKDKLENIAEEDRTL
IFYESTHRILDTLEDMQAVLGEERYIVLAREMTKTWETITGNTIKNLREWLLEDPNRTKGEMVLIVEGKPKSDNNDEISP
QAVKALELIAEELPLKKAAAIVAELYGYKKNALYQFGLAHLEK

Sequences:

>Translated_283_residues
MTDLTGILYIVATPIGNLQDITQRALETFAQVDLIAAEDTRHSGLLLSHYGIKKPFFALHDHNEQEKAHILVEKLKQGSN
IALISDAGTPLISDPGFHLVRQCREAGIRVVPLPGACAAITALCASGIASDRFCFEGFLPAKSKARKDKLENIAEEDRTL
IFYESTHRILDTLEDMQAVLGEERYIVLAREMTKTWETITGNTIKNLREWLLEDPNRTKGEMVLIVEGKPKSDNNDEISP
QAVKALELIAEELPLKKAAAIVAELYGYKKNALYQFGLAHLEK
>Mature_282_residues
TDLTGILYIVATPIGNLQDITQRALETFAQVDLIAAEDTRHSGLLLSHYGIKKPFFALHDHNEQEKAHILVEKLKQGSNI
ALISDAGTPLISDPGFHLVRQCREAGIRVVPLPGACAAITALCASGIASDRFCFEGFLPAKSKARKDKLENIAEEDRTLI
FYESTHRILDTLEDMQAVLGEERYIVLAREMTKTWETITGNTIKNLREWLLEDPNRTKGEMVLIVEGKPKSDNNDEISPQ
AVKALELIAEELPLKKAAAIVAELYGYKKNALYQFGLAHLEK

Specific function: Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA

COG id: COG0313

COG function: function code R; Predicted methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmI family

Homologues:

Organism=Escherichia coli, GI1789535, Length=273, Percent_Identity=68.1318681318681, Blast_Score=370, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMI_HAEIN (P45298)

Other databases:

- EMBL:   L42023
- PIR:   A64174
- RefSeq:   NP_439796.1
- ProteinModelPortal:   P45298
- SMR:   P45298
- GeneID:   950491
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1654
- NMPDR:   fig|71421.1.peg.1568
- TIGR:   HI_1654
- HOGENOM:   HBG529058
- OMA:   ASDRFCF
- ProtClustDB:   CLSK870184
- BioCyc:   HINF71421:HI_1654-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01877
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR008189
- InterPro:   IPR018063
- Gene3D:   G3DSA:3.40.1010.10
- TIGRFAMs:   TIGR00096

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: NA

Molecular weight: Translated: 31464; Mature: 31332

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS01296 RSMI

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTGILYIVATPIGNLQDITQRALETFAQVDLIAAEDTRHSGLLLSHYGIKKPFFALH
CCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECCCCCCCEEEEC
DHNEQEKAHILVEKLKQGSNIALISDAGTPLISDPGFHLVRQCREAGIRVVPLPGACAAI
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHH
TALCASGIASDRFCFEGFLPAKSKARKDKLENIAEEDRTLIFYESTHRILDTLEDMQAVL
HHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
GEERYIVLAREMTKTWETITGNTIKNLREWLLEDPNRTKGEMVLIVEGKPKSDNNDEISP
CCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCH
QAVKALELIAEELPLKKAAAIVAELYGYKKNALYQFGLAHLEK
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDLTGILYIVATPIGNLQDITQRALETFAQVDLIAAEDTRHSGLLLSHYGIKKPFFALH
CCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECCCCCCCEEEEC
DHNEQEKAHILVEKLKQGSNIALISDAGTPLISDPGFHLVRQCREAGIRVVPLPGACAAI
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHH
TALCASGIASDRFCFEGFLPAKSKARKDKLENIAEEDRTLIFYESTHRILDTLEDMQAVL
HHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
GEERYIVLAREMTKTWETITGNTIKNLREWLLEDPNRTKGEMVLIVEGKPKSDNNDEISP
CCCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCH
QAVKALELIAEELPLKKAAAIVAELYGYKKNALYQFGLAHLEK
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800