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The map label for this gene is truA [H]

Identifier: 16273533

GI number: 16273533

Start: 1709484

End: 1710293

Strand: Reverse

Name: truA [H]

Synonym: HI1644

Alternate gene names: 16273533

Gene position: 1710293-1709484 (Counterclockwise)

Preceding gene: 16273655

Following gene: 16273526

Centisome position: 93.45

GC content: 40.99

Gene sequence:

>810_bases
ATGAAAATTGCATTAGGCATTGAGTATAACGGGCAAAATTATTATGGTTGGCAGAGACAAGAAAAAGTCCGCAGTGTACA
AGAAGAATTAGAAAAGGCACTTTCTCACATTGCAAATGAAAAAATTGAAATATTTTGTGCTGGCAGAACGGATTCTGGCG
TAAGTGGAACGGGTCAGGTTGTTCATTTTGAAACCAATGCGGTTCGTCCAGAGAAGGCTTGGGCTTTTGGTACGAATGCT
CATTTACCTGATGACATTGCAGTGAGTTGGGCAAAACAAGTCGATGATGAATTCCATGCGAGATTTTCTGCAACAGCACG
CCGTTACCGCTATATTCTTTATTGTAATAAATTACGCTCTGCGATTTTAGCGGGAGGAATAACCCATTGTCATTTAGATT
TAGATGCGGAAAAAATGCATCAGGCAGGGCAATGTTTACTTGGCGAACAGGATTTTTCCTCTTTCCGTGCGGCGCAATGT
CAGTCTCATACGCCTTGGCGTAATGTGCATCATTTGAATGTGTCTCGTATCGGAAAATATATTATTGTTGATATTCAGGC
GAACGCTTTTGTGCATCATATGGTGCGCAATATTGTGGGAAGTTTGATTGAGGTTGGTACTGGAAATCAGCCGATTGAAT
GGATGCAATGGCTGCTTGAGCAGAAAAATCGTCAGCTTGCCGCACCAACAGCAAAACCCGATGGATTGTATTTGGTGGAT
GTGATTTATCCACAAAAGTTTGATATTCCTAAACGCCCAATTGGCCCTTTATTTTTAGAGGATGGTTTATTAAATCGTCC
TTTGAAATAA

Upstream 100 bases:

>100_bases
TCGCTTATTTTTGAATAAATTTTGGAGTGTTTTTATGCATTTGCGCTCCAAATAAACTTGGGCTACCATAACCGCACTTT
ATTAAGCCAAAAGGTAAAAA

Downstream 100 bases:

>100_bases
AGCGTAATTTCATCTTTTAAATAACCATATCTGAAAATATTCTTCATAAAAAAAGACCGCACTTTAAAAGTGCGGTCAAT
GTTAAGTGAATTTATATTAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKIALGIEYNGQNYYGWQRQEKVRSVQEELEKALSHIANEKIEIFCAGRTDSGVSGTGQVVHFETNAVRPEKAWAFGTNA
HLPDDIAVSWAKQVDDEFHARFSATARRYRYILYCNKLRSAILAGGITHCHLDLDAEKMHQAGQCLLGEQDFSSFRAAQC
QSHTPWRNVHHLNVSRIGKYIIVDIQANAFVHHMVRNIVGSLIEVGTGNQPIEWMQWLLEQKNRQLAAPTAKPDGLYLVD
VIYPQKFDIPKRPIGPLFLEDGLLNRPLK

Sequences:

>Translated_269_residues
MKIALGIEYNGQNYYGWQRQEKVRSVQEELEKALSHIANEKIEIFCAGRTDSGVSGTGQVVHFETNAVRPEKAWAFGTNA
HLPDDIAVSWAKQVDDEFHARFSATARRYRYILYCNKLRSAILAGGITHCHLDLDAEKMHQAGQCLLGEQDFSSFRAAQC
QSHTPWRNVHHLNVSRIGKYIIVDIQANAFVHHMVRNIVGSLIEVGTGNQPIEWMQWLLEQKNRQLAAPTAKPDGLYLVD
VIYPQKFDIPKRPIGPLFLEDGLLNRPLK
>Mature_269_residues
MKIALGIEYNGQNYYGWQRQEKVRSVQEELEKALSHIANEKIEIFCAGRTDSGVSGTGQVVHFETNAVRPEKAWAFGTNA
HLPDDIAVSWAKQVDDEFHARFSATARRYRYILYCNKLRSAILAGGITHCHLDLDAEKMHQAGQCLLGEQDFSSFRAAQC
QSHTPWRNVHHLNVSRIGKYIIVDIQANAFVHHMVRNIVGSLIEVGTGNQPIEWMQWLLEQKNRQLAAPTAKPDGLYLVD
VIYPQKFDIPKRPIGPLFLEDGLLNRPLK

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=69.2307692307692, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30530; Mature: 30530

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALGIEYNGQNYYGWQRQEKVRSVQEELEKALSHIANEKIEIFCAGRTDSGVSGTGQV
CEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCE
VHFETNAVRPEKAWAFGTNAHLPDDIAVSWAKQVDDEFHARFSATARRYRYILYCNKLRS
EEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHH
AILAGGITHCHLDLDAEKMHQAGQCLLGEQDFSSFRAAQCQSHTPWRNVHHLNVSRIGKY
HHHHCCCEEEEEECCHHHHHHHCCEECCCHHHHHHHHHHCCCCCCCCCEEEECHHHCCCE
IIVDIQANAFVHHMVRNIVGSLIEVGTGNQPIEWMQWLLEQKNRQLAAPTAKPDGLYLVD
EEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VIYPQKFDIPKRPIGPLFLEDGLLNRPLK
EECCCCCCCCCCCCCCCEECCCCCCCCCC
>Mature Secondary Structure
MKIALGIEYNGQNYYGWQRQEKVRSVQEELEKALSHIANEKIEIFCAGRTDSGVSGTGQV
CEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCE
VHFETNAVRPEKAWAFGTNAHLPDDIAVSWAKQVDDEFHARFSATARRYRYILYCNKLRS
EEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHH
AILAGGITHCHLDLDAEKMHQAGQCLLGEQDFSSFRAAQCQSHTPWRNVHHLNVSRIGKY
HHHHCCCEEEEEECCHHHHHHHCCEECCCHHHHHHHHHHCCCCCCCCCEEEECHHHCCCE
IIVDIQANAFVHHMVRNIVGSLIEVGTGNQPIEWMQWLLEQKNRQLAAPTAKPDGLYLVD
EEEEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VIYPQKFDIPKRPIGPLFLEDGLLNRPLK
EECCCCCCCCCCCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA