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The map label for this gene is purR [H]

Identifier: 16273524

GI number: 16273524

Start: 1697612

End: 1698622

Strand: Direct

Name: purR [H]

Synonym: HI1635

Alternate gene names: 16273524

Gene position: 1697612-1698622 (Clockwise)

Preceding gene: 16273522

Following gene: 16273525

Centisome position: 92.76

GC content: 38.87

Gene sequence:

>1011_bases
ATGGCAACAATTAAAGATGTGGCAAAAATGGCTGGTGTGTCCACCACAACCGTATCTCACGTAATTAATAAAACCCGTTT
CGTTGCAAAAGATACGGAAGAAGCGGTGTTATCCGCAATTAAACAACTTAATTATTCCCCTAGTGCTGTGGCACGCAGTT
TAAAAGTCAATACCACCAAATCCATTGGTATGATTGTGACAACCAGTGAAGCACCGTATTTTGCCGAAATAATCCATTCT
GTTGAAGAACATTGCTATCGACAAGGTTATTCTTTATTTTGTGTAACACACAAAATGGATCCTGAAAAAGTCAAAAATCA
TTTAGAAATGTTGGCAAAAAAAAGGGTCGATGGTTTGCTTGTAATGTGTTCTGAATATACTCAGGATTCATTGGATTTAC
TTTCATCATTTTCCACTATTCCAATGGTTGTAATGGATTGGGGCCCAAATGCGAATACCGATGTGATTGATGATCATAGT
TTTGATGGCGGTTATTTAGCAACCAAACATCTTATAGAGTGCGGTCATAAAAAAATCGGGATTATCTGCGGCGAACTCAA
TAAAACTACCGCAAGAACGCGTTATGAAGGCTTTGAAAAAGCAATGGAAGAAGCTAAATTAACAATCAATCCGTCTTGGG
TACTAGAGGGTGCATTTGAGCCTGAAGATGGCTATGAATGTATGAATCGTTTACTGACTCAAGAGAAATTACCAACCGCC
CTATTCTGCTGTAACGATGTGATGGCGCTCGGTGCAATATCAGCACTAACAGAAAAAGGCTTGCGTGTGCCAGAGGATAT
GTCAATTATCGGCTATGATGATATTCACGCCTCACGTTTCTACGCGCCACCATTAACCACCATTCATCAATCAAAACTAC
GTTTAGGCAGACAGGCAATAAATATTCTGTTAGAACGAATTACTCACAAAGATGAAGGTGTTCAACAATATAGTCGTATC
GATATAACCCCTGAGCTTATCATTCGAAAATCCGTTAAATCGATTTTATAA

Upstream 100 bases:

>100_bases
ATACGAAAAAATCACTTCTTTTTATAAAAAATTTGCAATAAAGATGACTAAATTCTCAACAAACCTTTATAATAGGAAAA
ATTTTTAGGGATTTAAATAT

Downstream 100 bases:

>100_bases
ACTTAGCAAAAATAGACCGCACTTTTTAAAAAATCCCTAAAAAGTGCGGGTCTTTTTTAGCTATTTTTTAGCTTTTCTTG
ATTATTTTCAAAATTTCTCT

Product: DNA-binding transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTKSIGMIVTTSEAPYFAEIIHS
VEEHCYRQGYSLFCVTHKMDPEKVKNHLEMLAKKRVDGLLVMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHS
FDGGYLATKHLIECGHKKIGIICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEKLPTA
LFCCNDVMALGAISALTEKGLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAINILLERITHKDEGVQQYSRI
DITPELIIRKSVKSIL

Sequences:

>Translated_336_residues
MATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTKSIGMIVTTSEAPYFAEIIHS
VEEHCYRQGYSLFCVTHKMDPEKVKNHLEMLAKKRVDGLLVMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHS
FDGGYLATKHLIECGHKKIGIICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEKLPTA
LFCCNDVMALGAISALTEKGLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAINILLERITHKDEGVQQYSRI
DITPELIIRKSVKSIL
>Mature_335_residues
ATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTKSIGMIVTTSEAPYFAEIIHSV
EEHCYRQGYSLFCVTHKMDPEKVKNHLEMLAKKRVDGLLVMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHSF
DGGYLATKHLIECGHKKIGIICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEKLPTAL
FCCNDVMALGAISALTEKGLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAINILLERITHKDEGVQQYSRID
ITPELIIRKSVKSIL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=55.9880239520958, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=47.289156626506, Blast_Score=320, Evalue=6e-89,
Organism=Escherichia coli, GI1790369, Length=336, Percent_Identity=33.3333333333333, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=35.6164383561644, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1786540, Length=304, Percent_Identity=32.5657894736842, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=29.1095890410959, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1788474, Length=308, Percent_Identity=31.1688311688312, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.3492063492064, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1789456, Length=317, Percent_Identity=29.6529968454259, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=25.8928571428571, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=26.3492063492064, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994940, Length=305, Percent_Identity=25.5737704918033, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790715, Length=317, Percent_Identity=25.5520504731861, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786268, Length=306, Percent_Identity=22.8758169934641, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790192, Length=244, Percent_Identity=25.4098360655738, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37509; Mature: 37378

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTK
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SIGMIVTTSEAPYFAEIIHSVEEHCYRQGYSLFCVTHKMDPEKVKNHLEMLAKKRVDGLL
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEE
VMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHSFDGGYLATKHLIECGHKKIG
EEEHHHCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
IICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEKLPTA
EEECCCCCHHHHHHHHHHHHHHHHCEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCHH
LFCCNDVMALGAISALTEKGLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAI
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NILLERITHKDEGVQQYSRIDITPELIIRKSVKSIL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
ATIKDVAKMAGVSTTTVSHVINKTRFVAKDTEEAVLSAIKQLNYSPSAVARSLKVNTTK
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SIGMIVTTSEAPYFAEIIHSVEEHCYRQGYSLFCVTHKMDPEKVKNHLEMLAKKRVDGLL
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCEE
VMCSEYTQDSLDLLSSFSTIPMVVMDWGPNANTDVIDDHSFDGGYLATKHLIECGHKKIG
EEEHHHCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEE
IICGELNKTTARTRYEGFEKAMEEAKLTINPSWVLEGAFEPEDGYECMNRLLTQEKLPTA
EEECCCCCHHHHHHHHHHHHHHHHCEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCHH
LFCCNDVMALGAISALTEKGLRVPEDMSIIGYDDIHASRFYAPPLTTIHQSKLRLGRQAI
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NILLERITHKDEGVQQYSRIDITPELIIRKSVKSIL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA