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The map label for this gene is purA [H]

Identifier: 16273522

GI number: 16273522

Start: 1695071

End: 1696369

Strand: Direct

Name: purA [H]

Synonym: HI1633

Alternate gene names: 16273522

Gene position: 1695071-1696369 (Clockwise)

Preceding gene: 16273521

Following gene: 16273524

Centisome position: 92.62

GC content: 41.26

Gene sequence:

>1299_bases
ATGGGAAAAAGCGTTGTTATCTTAGGCGCACAATGGGGCGATGAAGGTAAAGGAAAAATCGTTGATCTTTTGACGGATCG
CGTAAAATACGTAGTTCGTTATCAAGGCGGCCATAACGCAGGTCACACACTTATTATCAATGGCGAAAAAACCGTATTAC
GTTTAATTCCATCTGGTATGTTACATCCAAACGTAACTTGCTTAATTGGTAATGGCGTTGTGGTTTCGCCTGAAGCATTA
ATGAAAGAAATGGGCGAACTTGAAAGCCGAGGTATTAAAGTTCGTGAACGTTTATTAATTTCAGAAGCTTGTCCTTTGAT
TCTGCCTTATCATGTGGCTATGGATCACGCGCGCGAAGCAGCCCTTGGCAAAAAAGCTATAGGTACAACTGGTCGTGGTA
TTGGCCCAGCTTATGAAGATAAAGTAGCTCGTCGTGGTTTACGCGTAGGCGATTTATTTAATAAAGAAGCCTTTGCCGAA
AAACTCAAAAACATCCTTGAATACTATAATTTCCAATTGGTGAACTACTACAAAGTTGAACCTGTCGATTATCAAAAAAC
CTTAGATGATGTAATGGCAATTGCCGATGTAATCACAGGTATGGTGGCAGACATCACTACAATTCTTGATACTGCGCGCA
AAAATGGCGAACATATTTTATTTGAAGGCGCACAAGGGACAATGTTAGATATCGACCACGGTACTTATCCGTATGTAACC
AGCTCAAACACAACGGCTGGTGGTGTTGCAACAGGTTCTGGTTTTGGCCCGCGTAATCTTGATTATGTACTTGGTATCAT
TAAGGCTTACTGTACTCGTGTAGGTGGTGGCCCATTCACGACGGAATTATTTGATGATGTGGGTGCTGAAATTGCGCGTA
AAGGTAACGAATTTGGTGCGGTAACAGGTCGTCCACGTCGCTGTGGTTGGTTTGATGCCGTCGCCATTCGTCGTGCAATC
CAACTTAACTCTATTTCTGGTTTCTGTATGACTAAATTAGACGTATTAGATGGTTTTGATGAAGTAAAAATCTGTGTTGC
TTACAAAATGCCAAATGGCGAAATCGTTGAATATGCACCGTTAGCGGCGAAAGATTGGGAAGGTGTAGAACCGATTTATG
AAACATTACCTGGTTGGAAAGAAAATACTTTCCGTATCACCGATGTAAATAAATTACCACAAAACTGCATTAACTATATT
AAACGTATTGAAGAAGTGACTGGCGTACCAATTGATATTCTTTCAACTGGCCCTGATCGTGTGGAAACAATGATTTTACG
TGAACCGTTCGCTGCTTAA

Upstream 100 bases:

>100_bases
CACCACACTTTTTCTTTTATATCATTGAGATTAAAGCCGAAAAAGAGTAAAGTAAACGGCTGTTCATTTAACTAATTTAA
CCGAATAAAAGGCGGAAATT

Downstream 100 bases:

>100_bases
TTTCTTTTGATTTTCAGACCGCACTTTAAAAGTGCGGTCTTTTTTTATATTTTTTACTATTCTTCAATAGAACGTAATAA
TTCATTGATGCCCACTTTTC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGMLHPNVTCLIGNGVVVSPEAL
MKEMGELESRGIKVRERLLISEACPLILPYHVAMDHAREAALGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFNKEAFAE
KLKNILEYYNFQLVNYYKVEPVDYQKTLDDVMAIADVITGMVADITTILDTARKNGEHILFEGAQGTMLDIDHGTYPYVT
SSNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAIRRAI
QLNSISGFCMTKLDVLDGFDEVKICVAYKMPNGEIVEYAPLAAKDWEGVEPIYETLPGWKENTFRITDVNKLPQNCINYI
KRIEEVTGVPIDILSTGPDRVETMILREPFAA

Sequences:

>Translated_432_residues
MGKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGMLHPNVTCLIGNGVVVSPEAL
MKEMGELESRGIKVRERLLISEACPLILPYHVAMDHAREAALGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFNKEAFAE
KLKNILEYYNFQLVNYYKVEPVDYQKTLDDVMAIADVITGMVADITTILDTARKNGEHILFEGAQGTMLDIDHGTYPYVT
SSNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAIRRAI
QLNSISGFCMTKLDVLDGFDEVKICVAYKMPNGEIVEYAPLAAKDWEGVEPIYETLPGWKENTFRITDVNKLPQNCINYI
KRIEEVTGVPIDILSTGPDRVETMILREPFAA
>Mature_431_residues
GKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGMLHPNVTCLIGNGVVVSPEALM
KEMGELESRGIKVRERLLISEACPLILPYHVAMDHAREAALGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFNKEAFAEK
LKNILEYYNFQLVNYYKVEPVDYQKTLDDVMAIADVITGMVADITTILDTARKNGEHILFEGAQGTMLDIDHGTYPYVTS
SNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAIRRAIQ
LNSISGFCMTKLDVLDGFDEVKICVAYKMPNGEIVEYAPLAAKDWEGVEPIYETLPGWKENTFRITDVNKLPQNCINYIK
RIEEVTGVPIDILSTGPDRVETMILREPFAA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=41.2587412587413, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=40.6032482598608, Blast_Score=314, Evalue=8e-86,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=39.7984886649874, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=75.6944444444444, Blast_Score=697, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=41.8604651162791, Blast_Score=330, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=41.8604651162791, Blast_Score=330, Evalue=8e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=41.2442396313364, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=42.4170616113744, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47367; Mature: 47236

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGM
CCCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCCCC
LHPNVTCLIGNGVVVSPEALMKEMGELESRGIKVRERLLISEACPLILPYHVAMDHAREA
CCCCEEEEECCCEEECHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
ALGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFNKEAFAEKLKNILEYYNFQLVNYYKVE
HHCHHHCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHCEEEEEEEEEC
PVDYQKTLDDVMAIADVITGMVADITTILDTARKNGEHILFEGAQGTMLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEE
SSNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDDVGAEIARKGNEFGA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
VTGRPRRCGWFDAVAIRRAIQLNSISGFCMTKLDVLDGFDEVKICVAYKMPNGEIVEYAP
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECC
LAAKDWEGVEPIYETLPGWKENTFRITDVNKLPQNCINYIKRIEEVTGVPIDILSTGPDR
CCCCCCCCCCHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
VETMILREPFAA
HHHHHHCCCCCC
>Mature Secondary Structure 
GKSVVILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGM
CCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCCCC
LHPNVTCLIGNGVVVSPEALMKEMGELESRGIKVRERLLISEACPLILPYHVAMDHAREA
CCCCEEEEECCCEEECHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
ALGKKAIGTTGRGIGPAYEDKVARRGLRVGDLFNKEAFAEKLKNILEYYNFQLVNYYKVE
HHCHHHCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHCEEEEEEEEEC
PVDYQKTLDDVMAIADVITGMVADITTILDTARKNGEHILFEGAQGTMLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEE
SSNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGGGPFTTELFDDVGAEIARKGNEFGA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
VTGRPRRCGWFDAVAIRRAIQLNSISGFCMTKLDVLDGFDEVKICVAYKMPNGEIVEYAP
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEEEECC
LAAKDWEGVEPIYETLPGWKENTFRITDVNKLPQNCINYIKRIEEVTGVPIDILSTGPDR
CCCCCCCCCCHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHH
VETMILREPFAA
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA