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The map label for this gene is purK

Identifier: 16273506

GI number: 16273506

Start: 1683529

End: 1684617

Strand: Direct

Name: purK

Synonym: HI1616

Alternate gene names: 16273506

Gene position: 1683529-1684617 (Clockwise)

Preceding gene: 16273505

Following gene: 16273507

Centisome position: 91.99

GC content: 38.48

Gene sequence:

>1089_bases
ATGCAAAACTCCACCTTATACCCAACGGTTTATGTACTCGGCAACGGACAACTCGGCAGAATGTTACGTTACGCTGGTGC
ACCTTTAGATATTTATGTTGAACCCTTAGCCTTCAATGCACCAGTTTTTGACTTACCTGAAAATGCGATCATCACGGCGG
AAATTGAACGCTGGGAAAAAACGCCTTTGACTGAATTACTCGGCAATCATAAAAACTTCGTCAATCAACATATATTTGGA
TTATTGGCAGATCGTTTTACTCAAAAATCCTTACTTGATGAACTGAATCTTTCTACCTCGCCTTGGTGTTTATTAAAAGA
TAAAAATCAATGGAATGATCTTTTCCAAACTGTTGGCGAGAAAGTCGTCGTTAAACGTAGAACAGGTGGATATGATGGAC
GAGGTCAATGGATTATCAGAGATGAAAATAGAGCGGACATCACCGATGATTTATTTGGTGAAGTGATCGCAGAAAAATTT
ATTCCTTTCGATTATGAAGTATCTATTGTAGGCGCAAGGTTTAAAAATGGCGAAAAGCGTTTTTATCCCGTTACACATAA
CCTGCAACAAAACGGTATTTTGCGTTACAGTGTGGTTGATTGTGCTTTTCCTCAACAATCCGTACAACAAAAGCAAGCAG
AAACAATGCTCGGCAAAATTATGGATAAATTAGGCTATGTGGGTGTGATGGCAATGGAATGTTTTGTTGTAGGCGACAAA
TTACTGATTAACGAACTTGCGCCTCGTGTACATAACAGTGGACATTGGACACAACTAGGATGCTCGATTAGCCAATTTGA
ATTACATTTACGTGCGCTACTCAATTTGCCAACGCCTGAATTACAAACATTCGCCCCAAGTGTAATGATTAATTTAATCG
GCACCAATCATAACCCTAAGTGGCTTAACATACCTTTTGCACAACTTCATTGGTACGGTAAAGAAGTGAGAATTGGACGT
AAAGTTGGGCATATCAATCTTTCCCATCCTAATAAAGCGGTCATTATTCAACAATTAGAAAAGCTTTGCACTGAATTACC
AGAAGATTATCAATCAGGATTAAATTGGGCGATTGAAAAACTAAAATAA

Upstream 100 bases:

>100_bases
GGTATTGGACAATCCTGATCCACGCACATAAAAACATCTTTAGATCTGACCGCACTTTTAAAGTGCGGTTGTTTTTTTCG
TAAATTTAACGAGAAATATT

Downstream 100 bases:

>100_bases
TGTAAAAAATGACCGCACTTTTTGATGAATATTTAAAAATATCAAAGCGTGGTTAATTTTTATAAAATCTTTCTTGATAA
AATAAAATGACTGAAGTATA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MQNSTLYPTVYVLGNGQLGRMLRYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEKTPLTELLGNHKNFVNQHIFG
LLADRFTQKSLLDELNLSTSPWCLLKDKNQWNDLFQTVGEKVVVKRRTGGYDGRGQWIIRDENRADITDDLFGEVIAEKF
IPFDYEVSIVGARFKNGEKRFYPVTHNLQQNGILRYSVVDCAFPQQSVQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
LLINELAPRVHNSGHWTQLGCSISQFELHLRALLNLPTPELQTFAPSVMINLIGTNHNPKWLNIPFAQLHWYGKEVRIGR
KVGHINLSHPNKAVIIQQLEKLCTELPEDYQSGLNWAIEKLK

Sequences:

>Translated_362_residues
MQNSTLYPTVYVLGNGQLGRMLRYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEKTPLTELLGNHKNFVNQHIFG
LLADRFTQKSLLDELNLSTSPWCLLKDKNQWNDLFQTVGEKVVVKRRTGGYDGRGQWIIRDENRADITDDLFGEVIAEKF
IPFDYEVSIVGARFKNGEKRFYPVTHNLQQNGILRYSVVDCAFPQQSVQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
LLINELAPRVHNSGHWTQLGCSISQFELHLRALLNLPTPELQTFAPSVMINLIGTNHNPKWLNIPFAQLHWYGKEVRIGR
KVGHINLSHPNKAVIIQQLEKLCTELPEDYQSGLNWAIEKLK
>Mature_362_residues
MQNSTLYPTVYVLGNGQLGRMLRYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEKTPLTELLGNHKNFVNQHIFG
LLADRFTQKSLLDELNLSTSPWCLLKDKNQWNDLFQTVGEKVVVKRRTGGYDGRGQWIIRDENRADITDDLFGEVIAEKF
IPFDYEVSIVGARFKNGEKRFYPVTHNLQQNGILRYSVVDCAFPQQSVQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
LLINELAPRVHNSGHWTQLGCSISQFELHLRALLNLPTPELQTFAPSVMINLIGTNHNPKWLNIPFAQLHWYGKEVRIGR
KVGHINLSHPNKAVIIQQLEKLCTELPEDYQSGLNWAIEKLK

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786733, Length=353, Percent_Identity=55.5240793201133, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1788155, Length=254, Percent_Identity=27.1653543307087, Blast_Score=85, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324702, Length=384, Percent_Identity=29.4270833333333, Blast_Score=140, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURK_HAEIN (P43850)

Other databases:

- EMBL:   L42023
- PIR:   H64132
- RefSeq:   NP_439758.1
- ProteinModelPortal:   P43850
- GeneID:   950468
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1616
- NMPDR:   fig|71421.1.peg.1532
- TIGR:   HI_1616
- HOGENOM:   HBG516369
- OMA:   FSQLHWY
- ProtClustDB:   PRK06019
- BioCyc:   HINF71421:HI_1616-MONOMER
- BRENDA:   4.1.1.21
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF1
- TIGRFAMs:   TIGR01161

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.18

Molecular weight: Translated: 41274; Mature: 41274

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 85-85 BINDING 125-125 BINDING 136-136 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSTLYPTVYVLGNGQLGRMLRYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEK
CCCCEECEEEEEEECCCHHHHHHHCCCCEEEEEECEEECCCCEECCCCCEEEEEHHHCCC
TPLTELLGNHKNFVNQHIFGLLADRFTQKSLLDELNLSTSPWCLLKDKNQWNDLFQTVGE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCH
KVVVKRRTGGYDGRGQWIIRDENRADITDDLFGEVIAEKFIPFDYEVSIVGARFKNGEKR
HEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCE
FYPVTHNLQQNGILRYSVVDCAFPQQSVQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
EEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLINELAPRVHNSGHWTQLGCSISQFELHLRALLNLPTPELQTFAPSVMINLIGTNHNPK
HHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEEECCCCCC
WLNIPFAQLHWYGKEVRIGRKVGHINLSHPNKAVIIQQLEKLCTELPEDYQSGLNWAIEK
EEECCHHHHHCCCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LK
CC
>Mature Secondary Structure
MQNSTLYPTVYVLGNGQLGRMLRYAGAPLDIYVEPLAFNAPVFDLPENAIITAEIERWEK
CCCCEECEEEEEEECCCHHHHHHHCCCCEEEEEECEEECCCCEECCCCCEEEEEHHHCCC
TPLTELLGNHKNFVNQHIFGLLADRFTQKSLLDELNLSTSPWCLLKDKNQWNDLFQTVGE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCH
KVVVKRRTGGYDGRGQWIIRDENRADITDDLFGEVIAEKFIPFDYEVSIVGARFKNGEKR
HEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCE
FYPVTHNLQQNGILRYSVVDCAFPQQSVQQKQAETMLGKIMDKLGYVGVMAMECFVVGDK
EEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLINELAPRVHNSGHWTQLGCSISQFELHLRALLNLPTPELQTFAPSVMINLIGTNHNPK
HHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEEEECCCCCC
WLNIPFAQLHWYGKEVRIGRKVGHINLSHPNKAVIIQQLEKLCTELPEDYQSGLNWAIEK
EEECCHHHHHCCCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800