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The map label for this gene is purU

Identifier: 16273482

GI number: 16273482

Start: 1656239

End: 1657075

Strand: Direct

Name: purU

Synonym: HI1588

Alternate gene names: 16273482

Gene position: 1656239-1657075 (Clockwise)

Preceding gene: 16273481

Following gene: 16273483

Centisome position: 90.5

GC content: 34.65

Gene sequence:

>837_bases
ATGATCGAAAAAAAAATATTACTCACTGATTGCCCTGATGATAAAGGCTTAATCGCAAAAATAACTAATATTTGTTACAA
ACATCAACTAAATATTCTTCATAATAATGAATTTGTAGATTTTGAAACCAAACATTTCTTTATGCGCACAGAATTGGAAG
GTATTTTTAACGAAGCTACTTTATTAGAAGATCTAAAATATAGCTTACCAGAAGAAACAAATTGTCGATTAATTGGCACC
CAACGTAAGCGAATTGTAATTTTAGTCACCAAAGAAGCTCACTGCCTCGGCGATATTTTAATGAAAAATTATTATGGTGC
GCTTGATGTAGAAATTGCAGCCGTAATCGGCAATCACGATAATTTACGTGAACTTGTTGAACGTTTTAATATTCCATTTC
ATTTAGTTAGCCACGAAAACTTAACCCGTGTAGAACACGACAAATTACTGGCAGAAAAAATTGATGAATATACGCCAGAT
TATATTGTTCTCGCTAAATATATGCGTGTGCTAAACCCTGAATTTGTCGCACGTTATCCAAATCGAGTGATTAATATCCA
TCATTCATTCTTGCCTGCTTTTATTGGTGCCAAGCCTTATCAACAAGCCTATAAACGCGGTGTAAAAATTATTGGCGCAA
CGGCACATTTTATTAATAACGAATTAGATCAAGGCCCAATCATTATGCAAAATGTGATTAATGTGGATCATACTTATAAT
GCAGAAGCGATGATGCGTGCTGGCCGTGATGTGGAAAAAACCGTGTTAAGTCGCGCCCTTGATCTCGCTTTACACGATAG
AATTTTTGTTTATAAAAATAAAACGGTGGTGTTGTAA

Upstream 100 bases:

>100_bases
AGGTAAATCATTATCTGATTTCGAAATTTAATTTTTCTATTTGAAACTAGAACCTATTTTATGGGTTCTATTTTTTTATC
TAAACATAGCACACAACATT

Downstream 100 bases:

>100_bases
TGGAAAAAATTACTCTTGCACCAATAAGTGCGGTTGAAGGAACAATTAATTTACCAGGTTCTAAAAGTTTATCGAATCGC
GCATTACTTTTAGCCGCCTT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEATLLEDLKYSLPEETNCRLIGT
QRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHDNLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPD
YIVLAKYMRVLNPEFVARYPNRVINIHHSFLPAFIGAKPYQQAYKRGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL

Sequences:

>Translated_278_residues
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEATLLEDLKYSLPEETNCRLIGT
QRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHDNLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPD
YIVLAKYMRVLNPEFVARYPNRVINIHHSFLPAFIGAKPYQQAYKRGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
>Mature_278_residues
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEATLLEDLKYSLPEETNCRLIGT
QRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHDNLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPD
YIVLAKYMRVLNPEFVARYPNRVINIHHSFLPAFIGAKPYQQAYKRGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=28.1081081081081, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=28.1081081081081, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=28.1081081081081, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=72.5631768953069, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1788846, Length=154, Percent_Identity=33.7662337662338, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=217, Percent_Identity=31.3364055299539, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=26.5625, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURU_HAEIN (Q03432)

Other databases:

- EMBL:   L42023
- EMBL:   L04686
- PIR:   E64131
- RefSeq:   NP_439733.1
- ProteinModelPortal:   Q03432
- SMR:   Q03432
- GeneID:   950306
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1588
- NMPDR:   fig|71421.1.peg.1508
- TIGR:   HI_1588
- HOGENOM:   HBG286016
- OMA:   HQLNIIK
- ProtClustDB:   PRK06027
- BioCyc:   HINF71421:HI_1588-MONOMER
- BRENDA:   3.5.1.10
- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810
- Gene3D:   G3DSA:3.40.50.170
- PIRSF:   PIRSF036480
- PRINTS:   PR01575
- TIGRFAMs:   TIGR00655

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N; SSF53328 formyl_transf

EC number: =3.5.1.10

Molecular weight: Translated: 32174; Mature: 32174

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: ACT_SITE 223-223

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEAT
CCCCEEEEEECCCCCCHHHHHHHHHHHHHEEEEECCEEEEECHHHEEHHHHHHHHHHHHH
LLEDLKYSLPEETNCRLIGTQRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHD
HHHHHHHCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCCH
NLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPDYIVLAKYMRVLNPEFVARYP
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHCC
NRVINIHHSFLPAFIGAKPYQQAYKRGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
CCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCCCCCC
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
HHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCEEEC
>Mature Secondary Structure
MIEKKILLTDCPDDKGLIAKITNICYKHQLNILHNNEFVDFETKHFFMRTELEGIFNEAT
CCCCEEEEEECCCCCCHHHHHHHHHHHHHEEEEECCEEEEECHHHEEHHHHHHHHHHHHH
LLEDLKYSLPEETNCRLIGTQRKRIVILVTKEAHCLGDILMKNYYGALDVEIAAVIGNHD
HHHHHHHCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCCH
NLRELVERFNIPFHLVSHENLTRVEHDKLLAEKIDEYTPDYIVLAKYMRVLNPEFVARYP
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHCC
NRVINIHHSFLPAFIGAKPYQQAYKRGVKIIGATAHFINNELDQGPIIMQNVINVDHTYN
CCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCCCCCC
AEAMMRAGRDVEKTVLSRALDLALHDRIFVYKNKTVVL
HHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 8335255