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The map label for this gene is prfA [H]

Identifier: 16273461

GI number: 16273461

Start: 1630955

End: 1632040

Strand: Reverse

Name: prfA [H]

Synonym: HI1561

Alternate gene names: 16273461

Gene position: 1632040-1630955 (Counterclockwise)

Preceding gene: 16273462

Following gene: 16273460

Centisome position: 89.18

GC content: 40.61

Gene sequence:

>1086_bases
ATGATGAAAGATTCCATTATTGCAAAATTAGAAAGTTTAAAAGAACGCTATGAAGAGCTAGAAGCCTTATTGGGCGATGT
TTCTGTAATTAGTGATCAGGATAAATTCCGTGCGTATTCTAAAGAATATTCGCAACTTGAAGAAGTGGTGAAATGTTTTA
ATCGTTGGACGCAATTAAATCAAAATATTGAAGAAGCTGAAATATTGCTCGACGATCCTGAAATGAAAGAAATGGCACAA
ATGGAAATCGAAGAATCCAAAGCGGAAATTGAAGAAGTGGAACAACAACTTCAAATTCTTTTACTGCCGAAAGATCCTAA
TGATGAATATAACTGCTATTTAGAAATCCGAGCGGGAACAGGCGGTGATGAAGCGGGTATTTTTGCGGGTGATTTATTCC
GTATGTACAGTCGTTATGCTGAAAGTAAACGCTGGCGTGTAGAAATGCTAAGTGCTAACGAAAGTGAGCAAGGCGGTTAT
AAAGAGGTGATTGTTAAAGTCACTGGCGAAGGTGTGTATGGTCAGTTGAAGTTTGAGTCTGGTGGTCATCGTGTGCAACG
TGTACCAAAAACTGAAAGCCAAGGGCGTATCCACACGTCTGCTTGTACCGTTGCGGTAATGCCAGAATTGCCTGAATCTG
AAATGCCAGAAATTAATCCAGCAGATTTGCGTATTGATACTTATCGTTCATCAGGCGCTGGTGGTCAGCACGTAAATACA
ACGGATTCTGCGGTGCGTATTACACATATTCCAACAGGTATTGTGGTGGAATGTCAGGATGAGCGTTCACAGCACAAAAA
TAAAGCGAAAGCAATGTCAGTATTAGCGTCACGTATTGTTCAAGCAGAGCAAGAACGCCAAGCCGCAGAACAAACAGATA
TGCGTCGTAACTTATTAGGTTCGGGCGATCGTTCTGATAAAATTCGTACATATAATTATCCGCAAGGTCGAGTTACAGAT
CACCGTATCAATCTCACCATTTACCGTTTGGATGAAGTGATGAATGGTAAAATTGATGAACTGATTCAGCCGATTATTAC
CGAATATCAAGCGGATCAGTTGGCTGCATTGTCTGAACAGAATTAA

Upstream 100 bases:

>100_bases
AATCACAAATAAAGCGTCTGAGATTCTTAATTTCCTTTTCGCTGTCAATTTTCCCTAAAATCGGCTAGAATACGCCGATT
TTATTTTCGGTGACTTACAA

Downstream 100 bases:

>100_bases
TGATAGGGACATCTGATTTCAGATGTCCTTTTTAACTTTAAAAGGAAATAAAATGATCATTGAAAATCAAAAAGATGCTG
AATTTTCTTCTGCTTTCAAG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MMKDSIIAKLESLKERYEELEALLGDVSVISDQDKFRAYSKEYSQLEEVVKCFNRWTQLNQNIEEAEILLDDPEMKEMAQ
MEIEESKAEIEEVEQQLQILLLPKDPNDEYNCYLEIRAGTGGDEAGIFAGDLFRMYSRYAESKRWRVEMLSANESEQGGY
KEVIVKVTGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEINPADLRIDTYRSSGAGGQHVNT
TDSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEQERQAAEQTDMRRNLLGSGDRSDKIRTYNYPQGRVTD
HRINLTIYRLDEVMNGKIDELIQPIITEYQADQLAALSEQN

Sequences:

>Translated_361_residues
MMKDSIIAKLESLKERYEELEALLGDVSVISDQDKFRAYSKEYSQLEEVVKCFNRWTQLNQNIEEAEILLDDPEMKEMAQ
MEIEESKAEIEEVEQQLQILLLPKDPNDEYNCYLEIRAGTGGDEAGIFAGDLFRMYSRYAESKRWRVEMLSANESEQGGY
KEVIVKVTGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEINPADLRIDTYRSSGAGGQHVNT
TDSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEQERQAAEQTDMRRNLLGSGDRSDKIRTYNYPQGRVTD
HRINLTIYRLDEVMNGKIDELIQPIITEYQADQLAALSEQN
>Mature_361_residues
MMKDSIIAKLESLKERYEELEALLGDVSVISDQDKFRAYSKEYSQLEEVVKCFNRWTQLNQNIEEAEILLDDPEMKEMAQ
MEIEESKAEIEEVEQQLQILLLPKDPNDEYNCYLEIRAGTGGDEAGIFAGDLFRMYSRYAESKRWRVEMLSANESEQGGY
KEVIVKVTGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEINPADLRIDTYRSSGAGGQHVNT
TDSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEQERQAAEQTDMRRNLLGSGDRSDKIRTYNYPQGRVTD
HRINLTIYRLDEVMNGKIDELIQPIITEYQADQLAALSEQN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=305, Percent_Identity=47.5409836065574, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI34577120, Length=365, Percent_Identity=36.7123287671233, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI166795305, Length=210, Percent_Identity=47.6190476190476, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=73.2590529247911, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=41.0029498525074, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17542784, Length=291, Percent_Identity=36.426116838488, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=342, Percent_Identity=41.5204678362573, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI19921226, Length=359, Percent_Identity=37.883008356546, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41049; Mature: 41049

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKDSIIAKLESLKERYEELEALLGDVSVISDQDKFRAYSKEYSQLEEVVKCFNRWTQLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QNIEEAEILLDDPEMKEMAQMEIEESKAEIEEVEQQLQILLLPKDPNDEYNCYLEIRAGT
CCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEEEEEECC
GGDEAGIFAGDLFRMYSRYAESKRWRVEMLSANESEQGGYKEVIVKVTGEGVYGQLKFES
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEECCCEEEEEEECC
GGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEINPADLRIDTYRSSGAGGQHVNT
CCCHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
TDSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEQERQAAEQTDMRRNLLG
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGDRSDKIRTYNYPQGRVTDHRINLTIYRLDEVMNGKIDELIQPIITEYQADQLAALSEQ
CCCCCCCEEEEECCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
N
C
>Mature Secondary Structure
MMKDSIIAKLESLKERYEELEALLGDVSVISDQDKFRAYSKEYSQLEEVVKCFNRWTQLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QNIEEAEILLDDPEMKEMAQMEIEESKAEIEEVEQQLQILLLPKDPNDEYNCYLEIRAGT
CCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEEEEEECC
GGDEAGIFAGDLFRMYSRYAESKRWRVEMLSANESEQGGYKEVIVKVTGEGVYGQLKFES
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEECCCEEEEEEECC
GGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEINPADLRIDTYRSSGAGGQHVNT
CCCHHHCCCCCCCCCEEEECEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
TDSAVRITHIPTGIVVECQDERSQHKNKAKAMSVLASRIVQAEQERQAAEQTDMRRNLLG
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGDRSDKIRTYNYPQGRVTDHRINLTIYRLDEVMNGKIDELIQPIITEYQADQLAALSEQ
CCCCCCCEEEEECCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA