BacMap

Definition	Haemophilus influenzae Rd KW20 chromosome, complete genome.
Accession	NC_000907
Length	1,830,138

Click here to switch to the map view.

The map label for this gene is serA [C]

Identifier: 16273456

GI number: 16273456

Start: 1626905

End: 1627852

Strand: Reverse

Name: serA [C]

Synonym: HI1556

Alternate gene names: 16273456

Gene position: 1627852-1626905 (Counterclockwise)

Preceding gene: 16273457

Following gene: 16273455

Centisome position: 88.95

GC content: 39.35

Gene sequence:

>948_bases
ATGAAAATCGTATTTTTAGATAGCACGGCTATTCCTAAACATATTTCAATTCCTCGCCCAAGTTTTGAACATACTTGGAC
AGAATATGAACATACATCTGCTGAGCAAACGATTGAACGCGTAAAGGATGCAGATATTGTGATTACGAGCAAAGTGATTT
TTGATCGTGAAACTTTGCAACAACTGCCAAAATTAAAGCTGATTGCCATTACTGCGACAGGTACAAATAATGTTGATCTT
GTGGCTGCAGAAGAAATGGGCATCGCCGTGCGTAATGTGACAGGTTATTCCAGTACAACCGTGCCAGAACACGTAATCGG
TCTGATTTTTTCTTTAAAACACAGTTTGGCAGGTTGGTTACGCGATCAAACGGAAGCAAAATGGGCGGAAAGTAAACAAT
TTTGTTATTTTGATTATCCAATTACAGATGTGCGCGGTTCAACGTTGGGCGTATTTGGTAAAGGTTGCCTAGGAACAGAA
GTGGGGCGTTTGGCTAATGCCGTGGGAATGAAAGTGCTTTATGCAGAACATAAAGATGCCACTGTTTGCCGTGAAGGTTA
TACGCCTTTTGATGAAGTATTAAAACAAGCGGATATTGTGACGTTGCATTGCCCATTAACGGAAACAACAAAGGATTTAA
TTAATGCAGAAACCTTATCCAAAATGAAAAAAGGTGCATTTTTGATTAATACAGGGCGTGGGCCTTTGATTGATGAACTT
GCCTTAGTTGATGCGTTAAAAACGGGGCATTTAGGCGGAGCGGCTTTGGATGTAATGGTAAAAGAGCCACCAGAAAAAGA
TAATCCGTTAATTTTAGCTGCAAAAACGATGCCTAATTTAATTATCACACCACATATTGCTTGGGCGAGTGATAGTGCAG
TGACAACATTAGTTGGCAAAGTAATGCAAAACATCGAAGAATTTGTACAACAATTACATCAAAAATAA

Upstream 100 bases:

>100_bases
ATTTAGTAAAATCTTTTCCAGAATTGGATACCTCAATTTAAATTTCATCAAAAGTGCGGTTAATTTTGACCGCACTTTGT
ATATAAAAAAGGAGCAATTA

Downstream 100 bases:

>100_bases
TGAATTTTCCTATTTCTCTTTATATTGCACTACGTTATTGGCGTGCGAAAAGTGCGGATCGTTTTGGGCGATTAGTGACT
AATCTTGCAAGCCTGGGGAT

Product: glycerate dehydrogenase

Products: 3-Phosphohydroxypyruvate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKIVFLDSTAIPKHISIPRPSFEHTWTEYEHTSAEQTIERVKDADIVITSKVIFDRETLQQLPKLKLIAITATGTNNVDL
VAAEEMGIAVRNVTGYSSTTVPEHVIGLIFSLKHSLAGWLRDQTEAKWAESKQFCYFDYPITDVRGSTLGVFGKGCLGTE
VGRLANAVGMKVLYAEHKDATVCREGYTPFDEVLKQADIVTLHCPLTETTKDLINAETLSKMKKGAFLINTGRGPLIDEL
ALVDALKTGHLGGAALDVMVKEPPEKDNPLILAAKTMPNLIITPHIAWASDSAVTTLVGKVMQNIEEFVQQLHQK

Sequences:

>Translated_315_residues
MKIVFLDSTAIPKHISIPRPSFEHTWTEYEHTSAEQTIERVKDADIVITSKVIFDRETLQQLPKLKLIAITATGTNNVDL
VAAEEMGIAVRNVTGYSSTTVPEHVIGLIFSLKHSLAGWLRDQTEAKWAESKQFCYFDYPITDVRGSTLGVFGKGCLGTE
VGRLANAVGMKVLYAEHKDATVCREGYTPFDEVLKQADIVTLHCPLTETTKDLINAETLSKMKKGAFLINTGRGPLIDEL
ALVDALKTGHLGGAALDVMVKEPPEKDNPLILAAKTMPNLIITPHIAWASDSAVTTLVGKVMQNIEEFVQQLHQK
>Mature_315_residues
MKIVFLDSTAIPKHISIPRPSFEHTWTEYEHTSAEQTIERVKDADIVITSKVIFDRETLQQLPKLKLIAITATGTNNVDL
VAAEEMGIAVRNVTGYSSTTVPEHVIGLIFSLKHSLAGWLRDQTEAKWAESKQFCYFDYPITDVRGSTLGVFGKGCLGTE
VGRLANAVGMKVLYAEHKDATVCREGYTPFDEVLKQADIVTLHCPLTETTKDLINAETLSKMKKGAFLINTGRGPLIDEL
ALVDALKTGHLGGAALDVMVKEPPEKDNPLILAAKTMPNLIITPHIAWASDSAVTTLVGKVMQNIEEFVQQLHQK

Specific function: Serine biosynthesis; first step. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Homologues:

Organism=Homo sapiens, GI61743967, Length=268, Percent_Identity=34.7014925373134, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI4557497, Length=268, Percent_Identity=34.7014925373134, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI145580578, Length=252, Percent_Identity=36.9047619047619, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI4557499, Length=252, Percent_Identity=36.9047619047619, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI145580575, Length=252, Percent_Identity=36.9047619047619, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI23308577, Length=271, Percent_Identity=30.6273062730627, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI6912396, Length=239, Percent_Identity=26.7782426778243, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1789279, Length=262, Percent_Identity=32.0610687022901, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787645, Length=264, Percent_Identity=30.3030303030303, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082289, Length=289, Percent_Identity=30.4498269896194, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788660, Length=231, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25147481, Length=261, Percent_Identity=33.7164750957854, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17532191, Length=258, Percent_Identity=33.7209302325581, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=290, Percent_Identity=32.7586206896552, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320925, Length=290, Percent_Identity=32.7586206896552, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324055, Length=256, Percent_Identity=33.203125, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=266, Percent_Identity=31.203007518797, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324980, Length=90, Percent_Identity=37.7777777777778, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646446, Length=253, Percent_Identity=36.7588932806324, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24646448, Length=253, Percent_Identity=36.7588932806324, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24646452, Length=253, Percent_Identity=36.7588932806324, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24646450, Length=253, Percent_Identity=36.7588932806324, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI62472511, Length=253, Percent_Identity=36.7588932806324, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI19921140, Length=268, Percent_Identity=32.089552238806, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28574286, Length=264, Percent_Identity=28.030303030303, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571528, Length=275, Percent_Identity=29.0909090909091, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI45552429, Length=235, Percent_Identity=26.8085106382979, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45551003, Length=235, Percent_Identity=26.8085106382979, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585516, Length=304, Percent_Identity=24.3421052631579, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585514, Length=235, Percent_Identity=26.8085106382979, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574282, Length=235, Percent_Identity=26.8085106382979, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574284, Length=235, Percent_Identity=26.8085106382979, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): Y1556_HAEIN (P45250)

Other databases:

- EMBL:   L42023
- PIR:   F64129
- RefSeq:   NP_439705.1
- ProteinModelPortal:   P45250
- SMR:   P45250
- GeneID:   950416
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1556
- NMPDR:   fig|71421.1.peg.1483
- TIGR:   HI_1556
- HOGENOM:   HBG731446
- OMA:   ANAVGMK
- ProtClustDB:   PRK06932
- BioCyc:   HINF71421:HI_1556-MONOMER
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: 1.1.1.95 [C]

Molecular weight: Translated: 34636; Mature: 34636

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 233-233 ACT_SITE 262-262 ACT_SITE 285-285 BINDING 73-73 BINDING 257-257

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVFLDSTAIPKHISIPRPSFEHTWTEYEHTSAEQTIERVKDADIVITSKVIFDRETLQ
CEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCEEEEEHHHHCHHHHH
QLPKLKLIAITATGTNNVDLVAAEEMGIAVRNVTGYSSTTVPEHVIGLIFSLKHSLAGWL
HCCCEEEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RDQTEAKWAESKQFCYFDYPITDVRGSTLGVFGKGCLGTEVGRLANAVGMKVLYAEHKDA
HHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCC
TVCREGYTPFDEVLKQADIVTLHCPLTETTKDLINAETLSKMKKGAFLINTGRGPLIDEL
HHHHCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHH
ALVDALKTGHLGGAALDVMVKEPPEKDNPLILAAKTMPNLIITPHIAWASDSAVTTLVGK
HHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCEEEECCEECCCCCHHHHHHHH
VMQNIEEFVQQLHQK
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVFLDSTAIPKHISIPRPSFEHTWTEYEHTSAEQTIERVKDADIVITSKVIFDRETLQ
CEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCEEEEEHHHHCHHHHH
QLPKLKLIAITATGTNNVDLVAAEEMGIAVRNVTGYSSTTVPEHVIGLIFSLKHSLAGWL
HCCCEEEEEEEECCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RDQTEAKWAESKQFCYFDYPITDVRGSTLGVFGKGCLGTEVGRLANAVGMKVLYAEHKDA
HHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCC
TVCREGYTPFDEVLKQADIVTLHCPLTETTKDLINAETLSKMKKGAFLINTGRGPLIDEL
HHHHCCCCCHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHH
ALVDALKTGHLGGAALDVMVKEPPEKDNPLILAAKTMPNLIITPHIAWASDSAVTTLVGK
HHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCEEEECCEECCCCCHHHHHHHH
VMQNIEEFVQQLHQK
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-Phospho-D-glycerate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 3-Phospho-D-glycerate + Nicotinamide adenine dinucleotide --> 3-Phosphohydroxypyruvate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023