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The map label for this gene is trpG

Identifier: 16273297

GI number: 16273297

Start: 1484097

End: 1484678

Strand: Direct

Name: trpG

Synonym: HI1388

Alternate gene names: 16273297

Gene position: 1484097-1484678 (Clockwise)

Preceding gene: 16273296

Following gene: 16273298

Centisome position: 81.09

GC content: 38.66

Gene sequence:

>582_bases
ATGGCTAATATTTTATTTTTAGATAACTTCGATTCCTTCACTTACAATCTTGTGGATCAATTTCGTGTGTTGGGTCACAA
CGTCACAATTTACCGCAATGACTGCGACTTAGAAAAACTGGTAGAAACCGCACTCAATACACCTGATACCATTCTTGCAC
TTTCTCCAGGGCCAGGAACGCCATCAGAAGCTGGGATTTTGCTACCATTAATCGAACGCTTAAAAAATCAAGTGCCGATT
ATTGGAGTTTGTTTAGGTCATCAAGCGTTGATTCAAGCCTTTGGTGGTAAGGTTGTTCACGCTGGCGAAGTGTTGCATGG
TAAAGTTTCTCGTATATCCCATGATAACGAAGCTATGTTTAAAGATCTTGCAAATCCAATGCCTGTGGCGCGTTATCATT
CTTTAATGGGACAAGATTTACCAAAAGAATTTATCGTCAATGCGGAATATAATGGCATTATAATGGCAATTCGTCATCGC
GATTTACCCATTTGCGCATTCCAATTTCACCCTGAAAGTATTTTAACTGTGCAAGGTTCGCAATTATTACAACAATCAAT
TGAATGGTTGTTAAATAGATAA

Upstream 100 bases:

>100_bases
TGATCCACAAATGGAAGCTGATGAAACACGCCACAAAGCCGCGGCTGTCTTAAAAGCAATTCGTCAAGTTAATACACAAG
CAAAATAAGGAAGAATTATT

Downstream 100 bases:

>100_bases
GACATCAAAAAGTGCGGTAATTTTTAACCGCACTTTAAAGGAGAAAAAATGATCACTGTATTCGGACTTAAATCCAAACT
CGCACCACGTCGTGAAAAAC

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MANILFLDNFDSFTYNLVDQFRVLGHNVTIYRNDCDLEKLVETALNTPDTILALSPGPGTPSEAGILLPLIERLKNQVPI
IGVCLGHQALIQAFGGKVVHAGEVLHGKVSRISHDNEAMFKDLANPMPVARYHSLMGQDLPKEFIVNAEYNGIIMAIRHR
DLPICAFQFHPESILTVQGSQLLQQSIEWLLNR

Sequences:

>Translated_193_residues
MANILFLDNFDSFTYNLVDQFRVLGHNVTIYRNDCDLEKLVETALNTPDTILALSPGPGTPSEAGILLPLIERLKNQVPI
IGVCLGHQALIQAFGGKVVHAGEVLHGKVSRISHDNEAMFKDLANPMPVARYHSLMGQDLPKEFIVNAEYNGIIMAIRHR
DLPICAFQFHPESILTVQGSQLLQQSIEWLLNR
>Mature_192_residues
ANILFLDNFDSFTYNLVDQFRVLGHNVTIYRNDCDLEKLVETALNTPDTILALSPGPGTPSEAGILLPLIERLKNQVPII
GVCLGHQALIQAFGGKVVHAGEVLHGKVSRISHDNEAMFKDLANPMPVARYHSLMGQDLPKEFIVNAEYNGIIMAIRHRD
LPICAFQFHPESILTVQGSQLLQQSIEWLLNR

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1787517, Length=189, Percent_Identity=57.1428571428571, Blast_Score=244, Evalue=3e-66,
Organism=Escherichia coli, GI1789760, Length=183, Percent_Identity=51.3661202185792, Blast_Score=169, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322638, Length=186, Percent_Identity=42.4731182795699, Blast_Score=154, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6324361, Length=208, Percent_Identity=28.3653846153846, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPG_HAEIN (P71381)

Other databases:

- EMBL:   L42023
- PIR:   D64121
- RefSeq:   NP_439540.1
- ProteinModelPortal:   P71381
- SMR:   P71381
- GeneID:   950293
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1388
- NMPDR:   fig|71421.1.peg.1324
- TIGR:   HI_1388
- HOGENOM:   HBG292341
- OMA:   NIVMAIR
- ProtClustDB:   PRK05670
- BioCyc:   HINF71421:HI_1388-MONOMER
- BRENDA:   4.1.3.27
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00566

Pfam domain/function: PF00117 GATase

EC number: =4.1.3.27

Molecular weight: Translated: 21509; Mature: 21378

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 84-84 ACT_SITE 170-170 ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANILFLDNFDSFTYNLVDQFRVLGHNVTIYRNDCDLEKLVETALNTPDTILALSPGPGT
CCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCC
PSEAGILLPLIERLKNQVPIIGVCLGHQALIQAFGGKVVHAGEVLHGKVSRISHDNEAMF
CCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEECCHHHHHHHHHCCCCCHHHH
KDLANPMPVARYHSLMGQDLPKEFIVNAEYNGIIMAIRHRDLPICAFQFHPESILTVQGS
HHCCCCCCHHHHHHHHCCCCCHHHEECCCCCCEEEEEECCCCCEEEEEECCCEEEEECHH
QLLQQSIEWLLNR
HHHHHHHHHHHCC
>Mature Secondary Structure 
ANILFLDNFDSFTYNLVDQFRVLGHNVTIYRNDCDLEKLVETALNTPDTILALSPGPGT
CEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCC
PSEAGILLPLIERLKNQVPIIGVCLGHQALIQAFGGKVVHAGEVLHGKVSRISHDNEAMF
CCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEECCHHHHHHHHHCCCCCHHHH
KDLANPMPVARYHSLMGQDLPKEFIVNAEYNGIIMAIRHRDLPICAFQFHPESILTVQGS
HHCCCCCCHHHHHHHHCCCCCHHHEECCCCCCEEEEEECCCCCEEEEEECCCEEEEECHH
QLLQQSIEWLLNR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800