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The map label for this gene is trpE

Identifier: 16273296

GI number: 16273296

Start: 1482528

End: 1484084

Strand: Direct

Name: trpE

Synonym: HI1387

Alternate gene names: 16273296

Gene position: 1482528-1484084 (Clockwise)

Preceding gene: 16273295

Following gene: 16273297

Centisome position: 81.01

GC content: 39.24

Gene sequence:

>1557_bases
ATGAACATTCAAACTCAAGCCTTTATTGCGGTGACAGCTCAACCCATTCCCTATTATGCTGACACCACCGCTATTTTTAA
TACACTTTGTCAATCCAATTCAAATTCTCTTCTACTCGACTCTGCCGAAATCGGTAGTAAAAACAGTTTACAAAGCCTTA
TCTTAGTCAATGCTGCGGTTAAAATAACGTGCTTAGGTCACAACGTCACATTTAAAGCTTTAAATAATAATGGAAAACAA
GTTTTAAAAGAAATTCATCCAAAATTAACCGCACTTGGAAAAGTAAGTGCGGTCAATTTTGAAGACGAATTTTCTGTACA
ATTTTTGCCACTTGATAATCAACTTGATGAAGATAGTAAATTACAGTCTGCCACTATTTTTGATGGGTTGCGTGTTATTT
CTAGTCTTTATCAACATAGCCAAACGCCTATTTTCTTAGGCGGATTATTTGCTTACGATCTCGTCGCAAATTTTATTCCA
ATGGATGGCATTACATTAAAAAATGATGGTATCAACTGCCCTGATTACAGTTTCTATCTTGCCGAACACCTCATTACTAT
CGACCATCAAAATCAACAAGCAACACTGAAAAGTTTCTGTTTTGCTCAAGAAGAACAAGTTAATATTGCGAAAACCTCCC
TTTCCATCGCACAAAAACTAAAAAATATTGATCACGTTCTTTCTATTAAAGCGGCAAGTGATGAAGTTAAAACAAACTTT
GACGATCCTGAATTTACTGGTATCGTAAAAGCCTTAAAACACCATATTAATATTGGAGATGTTTTCCAAATTGTGCCTTC
TCGCCGTTTCTCCCTTGCTTGCCCGAATACATTAGCCAGTTACGCTCAACTCAAACAAAATAATCCAAGCCCTTATATGT
TCTATATGAACGATGAAGACTTTATTTTGTTTGGTGCATCGCCAGAAAGTGCGCTGAAATACGCGCCAGAAAATCGTCAG
CTAGAAATTTACCCTATCGCAGGTTCACGCCCTCGTGGTTTTGATGCTCACGGCAACATTGATCCTGAATTGGATGCGCG
TTTAGAACTAGAACTTCGCCTTGATCATAAAGAACAAGCAGAACATTTAATGTTAGTTGATTTAGCGCGTAACGACATTG
CTCGAGTTTGCCAAAGTGGCACCCGTAAAGTGGCAGAATTAATGCAAGTGGATCGCTATTCTCACATCATGCATTTAGTG
TCTCGTGTGGTGGGTAAATTACGCCCTGAATTAGATGCACTTCACGCCTATCAAGCCTGTATGAATATGGGCACATTAAC
TGGTGCGCCTAAAATTAAAGCGATGCAGCTTATTTATCAATTTGAGCAGCAAAAACGCCATAGTTACGGTGGTGCAGTGG
GTTATCTCACGTCCGATGGACATTTTGATACCTGTATCGTGATCCGTTCTGCTTTTGTACAAAATGGAATGGCCCATGTA
CAAGCAGGTTGCGGCGAAGTGTTGGATTCTGATCCACAAATGGAAGCTGATGAAACACGCCACAAAGCCGCGGCTGTCTT
AAAAGCAATTCGTCAAGTTAATACACAAGCAAAATAA

Upstream 100 bases:

>100_bases
ACAATTTTTAATCAAAAAGTATAAAAAATAAAAATCAGGCTTGACTTATTCCTTTTGCACTAGTTTAATAGTACAAAATT
TTCCAAAAGGATAAATAAAT

Downstream 100 bases:

>100_bases
GGAAGAATTATTATGGCTAATATTTTATTTTTAGATAACTTCGATTCCTTCACTTACAATCTTGTGGATCAATTTCGTGT
GTTGGGTCACAACGTCACAA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MNIQTQAFIAVTAQPIPYYADTTAIFNTLCQSNSNSLLLDSAEIGSKNSLQSLILVNAAVKITCLGHNVTFKALNNNGKQ
VLKEIHPKLTALGKVSAVNFEDEFSVQFLPLDNQLDEDSKLQSATIFDGLRVISSLYQHSQTPIFLGGLFAYDLVANFIP
MDGITLKNDGINCPDYSFYLAEHLITIDHQNQQATLKSFCFAQEEQVNIAKTSLSIAQKLKNIDHVLSIKAASDEVKTNF
DDPEFTGIVKALKHHINIGDVFQIVPSRRFSLACPNTLASYAQLKQNNPSPYMFYMNDEDFILFGASPESALKYAPENRQ
LEIYPIAGSRPRGFDAHGNIDPELDARLELELRLDHKEQAEHLMLVDLARNDIARVCQSGTRKVAELMQVDRYSHIMHLV
SRVVGKLRPELDALHAYQACMNMGTLTGAPKIKAMQLIYQFEQQKRHSYGGAVGYLTSDGHFDTCIVIRSAFVQNGMAHV
QAGCGEVLDSDPQMEADETRHKAAAVLKAIRQVNTQAK

Sequences:

>Translated_518_residues
MNIQTQAFIAVTAQPIPYYADTTAIFNTLCQSNSNSLLLDSAEIGSKNSLQSLILVNAAVKITCLGHNVTFKALNNNGKQ
VLKEIHPKLTALGKVSAVNFEDEFSVQFLPLDNQLDEDSKLQSATIFDGLRVISSLYQHSQTPIFLGGLFAYDLVANFIP
MDGITLKNDGINCPDYSFYLAEHLITIDHQNQQATLKSFCFAQEEQVNIAKTSLSIAQKLKNIDHVLSIKAASDEVKTNF
DDPEFTGIVKALKHHINIGDVFQIVPSRRFSLACPNTLASYAQLKQNNPSPYMFYMNDEDFILFGASPESALKYAPENRQ
LEIYPIAGSRPRGFDAHGNIDPELDARLELELRLDHKEQAEHLMLVDLARNDIARVCQSGTRKVAELMQVDRYSHIMHLV
SRVVGKLRPELDALHAYQACMNMGTLTGAPKIKAMQLIYQFEQQKRHSYGGAVGYLTSDGHFDTCIVIRSAFVQNGMAHV
QAGCGEVLDSDPQMEADETRHKAAAVLKAIRQVNTQAK
>Mature_518_residues
MNIQTQAFIAVTAQPIPYYADTTAIFNTLCQSNSNSLLLDSAEIGSKNSLQSLILVNAAVKITCLGHNVTFKALNNNGKQ
VLKEIHPKLTALGKVSAVNFEDEFSVQFLPLDNQLDEDSKLQSATIFDGLRVISSLYQHSQTPIFLGGLFAYDLVANFIP
MDGITLKNDGINCPDYSFYLAEHLITIDHQNQQATLKSFCFAQEEQVNIAKTSLSIAQKLKNIDHVLSIKAASDEVKTNF
DDPEFTGIVKALKHHINIGDVFQIVPSRRFSLACPNTLASYAQLKQNNPSPYMFYMNDEDFILFGASPESALKYAPENRQ
LEIYPIAGSRPRGFDAHGNIDPELDARLELELRLDHKEQAEHLMLVDLARNDIARVCQSGTRKVAELMQVDRYSHIMHLV
SRVVGKLRPELDALHAYQACMNMGTLTGAPKIKAMQLIYQFEQQKRHSYGGAVGYLTSDGHFDTCIVIRSAFVQNGMAHV
QAGCGEVLDSDPQMEADETRHKAAAVLKAIRQVNTQAK

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family

Homologues:

Organism=Escherichia coli, GI1787518, Length=499, Percent_Identity=52.3046092184369, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1788114, Length=406, Percent_Identity=29.3103448275862, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI87082077, Length=271, Percent_Identity=28.0442804428044, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786809, Length=228, Percent_Identity=30.2631578947368, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=379, Percent_Identity=36.6754617414248, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324361, Length=391, Percent_Identity=24.2966751918159, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPE_HAEIN (P43761)

Other databases:

- EMBL:   L42023
- PIR:   C64121
- RefSeq:   NP_439539.1
- ProteinModelPortal:   P43761
- SMR:   P43761
- GeneID:   950300
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1387
- NMPDR:   fig|71421.1.peg.1323
- TIGR:   HI_1387
- HOGENOM:   HBG507440
- OMA:   HKELAEH
- ProtClustDB:   PRK13564
- BioCyc:   HINF71421:HI_1387-MONOMER
- BRENDA:   4.1.3.27
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR00565

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =4.1.3.27

Molecular weight: Translated: 57522; Mature: 57522

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQTQAFIAVTAQPIPYYADTTAIFNTLCQSNSNSLLLDSAEIGSKNSLQSLILVNAAV
CCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHEEEEEE
KITCLGHNVTFKALNNNGKQVLKEIHPKLTALGKVSAVNFEDEFSVQFLPLDNQLDEDSK
EEEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCCCHH
LQSATIFDGLRVISSLYQHSQTPIFLGGLFAYDLVANFIPMDGITLKNDGINCPDYSFYL
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHH
AEHLITIDHQNQQATLKSFCFAQEEQVNIAKTSLSIAQKLKNIDHVLSIKAASDEVKTNF
HHHEEEEECCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCHHHHEEEECCCCHHHCCC
DDPEFTGIVKALKHHINIGDVFQIVPSRRFSLACPNTLASYAQLKQNNPSPYMFYMNDED
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEECCCC
FILFGASPESALKYAPENRQLEIYPIAGSRPRGFDAHGNIDPELDARLELELRLDHKEQA
EEEECCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHH
EHLMLVDLARNDIARVCQSGTRKVAELMQVDRYSHIMHLVSRVVGKLRPELDALHAYQAC
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MNMGTLTGAPKIKAMQLIYQFEQQKRHSYGGAVGYLTSDGHFDTCIVIRSAFVQNGMAHV
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHH
QAGCGEVLDSDPQMEADETRHKAAAVLKAIRQVNTQAK
HHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNIQTQAFIAVTAQPIPYYADTTAIFNTLCQSNSNSLLLDSAEIGSKNSLQSLILVNAAV
CCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHEEEEEE
KITCLGHNVTFKALNNNGKQVLKEIHPKLTALGKVSAVNFEDEFSVQFLPLDNQLDEDSK
EEEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCCCCHH
LQSATIFDGLRVISSLYQHSQTPIFLGGLFAYDLVANFIPMDGITLKNDGINCPDYSFYL
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHH
AEHLITIDHQNQQATLKSFCFAQEEQVNIAKTSLSIAQKLKNIDHVLSIKAASDEVKTNF
HHHEEEEECCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCHHHHEEEECCCCHHHCCC
DDPEFTGIVKALKHHINIGDVFQIVPSRRFSLACPNTLASYAQLKQNNPSPYMFYMNDED
CCHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEECCCC
FILFGASPESALKYAPENRQLEIYPIAGSRPRGFDAHGNIDPELDARLELELRLDHKEQA
EEEECCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHH
EHLMLVDLARNDIARVCQSGTRKVAELMQVDRYSHIMHLVSRVVGKLRPELDALHAYQAC
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MNMGTLTGAPKIKAMQLIYQFEQQKRHSYGGAVGYLTSDGHFDTCIVIRSAFVQNGMAHV
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHH
QAGCGEVLDSDPQMEADETRHKAAAVLKAIRQVNTQAK
HHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800