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The map label for this gene is rrmJ [H]

Identifier: 16273244

GI number: 16273244

Start: 1410002

End: 1410631

Strand: Direct

Name: rrmJ [H]

Synonym: HI1334

Alternate gene names: 16273244

Gene position: 1410002-1410631 (Clockwise)

Preceding gene: 16273242

Following gene: 16273245

Centisome position: 77.04

GC content: 39.37

Gene sequence:

>630_bases
ATGGGAAAGAAAAAACGTTCAGCGAGTTCTTCTCGTTGGCTAAATGAACATTTTAGTGATCAATTTGTGCAAAAAGCACA
TAAGCAAAAGTTGCGATCACGCGCTTATTTTAAGATTGATGAAATTCAGCAAACGGATAAATTATTTAAACAGGGAATGA
CCGTGGTCGATCTCGGTGCCGCACCAGGCGGTTGGTCACAATACGTAGTAAGCCAAATTGGTGGCAAAGGTCGAGTGATT
GCTTGTGATATTTTAGAAATGGATCCCATTGTAGGCGTTGATTTTCTACAAGGTGATTTCCGTGATGAAAATGTTTTGAA
TGCTTTATTAGCACGCGTAGGCGAAGATAAAGTTGATGTTGTGATGTCTGATATGGCACCAAATTTTAGTGGAATGCCAT
CAGTGGATATTCCACGCGCAATGTATTTGGTCGAGCTTGCTTTAGATATGTGTAAACAAGTCTTGGCAAGCAAAGGAAGT
TTTGTTGTTAAAGTCTTTCAAGGGGAAGGCTTTGATGAATATTTAAGAGAAATTCGTTCTTTATTTGATGTGGTAAAAGT
GCGTAAGCCTGAAGCATCACGTGGACGATCTCGTGAAGTATATATTGTGGCAACAGGCTATAAAGGATAA

Upstream 100 bases:

>100_bases
TGATTTTCTCAATGTTTTTTACTGATTTCAATTAGCTTCGGCTTGAATTTGACAGATTGTACCCAAATATAGGCGAAACG
ACTACAAAAAATAGAAGATT

Downstream 100 bases:

>100_bases
TTGCTTTGATAGCTTTTCATTGCTAATGATGTGTAGTATCTTGTGACGAAATTTTTTAACTTAGGAAAGGTAGGTTAGAC
TTTGAACGATATGGTCAAAA

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MGKKKRSASSSRWLNEHFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGAAPGGWSQYVVSQIGGKGRVI
ACDILEMDPIVGVDFLQGDFRDENVLNALLARVGEDKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLASKGS
FVVKVFQGEGFDEYLREIRSLFDVVKVRKPEASRGRSREVYIVATGYKG

Sequences:

>Translated_209_residues
MGKKKRSASSSRWLNEHFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGAAPGGWSQYVVSQIGGKGRVI
ACDILEMDPIVGVDFLQGDFRDENVLNALLARVGEDKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLASKGS
FVVKVFQGEGFDEYLREIRSLFDVVKVRKPEASRGRSREVYIVATGYKG
>Mature_208_residues
GKKKRSASSSRWLNEHFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGAAPGGWSQYVVSQIGGKGRVIA
CDILEMDPIVGVDFLQGDFRDENVLNALLARVGEDKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLASKGSF
VVKVFQGEGFDEYLREIRSLFDVVKVRKPEASRGRSREVYIVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=215, Percent_Identity=33.4883720930233, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI194097365, Length=207, Percent_Identity=31.8840579710145, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=32.6315789473684, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=32.6315789473684, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=32.6315789473684, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=81.7307692307692, Blast_Score=350, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=32.2727272727273, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=29.8429319371728, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71987550, Length=208, Percent_Identity=30.2884615384615, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71987561, Length=208, Percent_Identity=30.2884615384615, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=30.6878306878307, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319535, Length=201, Percent_Identity=32.8358208955224, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=210, Percent_Identity=29.5238095238095, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321302, Length=96, Percent_Identity=37.5, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21356387, Length=215, Percent_Identity=32.5581395348837, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI18859957, Length=207, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=33.160621761658, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=27.7777777777778, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR004512
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 23408; Mature: 23277

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKKRSASSSRWLNEHFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECC
APGGWSQYVVSQIGGKGRVIACDILEMDPIVGVDFLQGDFRDENVLNALLARVGEDKVDV
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHHH
VMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLASKGSFVVKVFQGEGFDEYLREIRS
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
LFDVVKVRKPEASRGRSREVYIVATGYKG
HHHHHHHCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
GKKKRSASSSRWLNEHFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECC
APGGWSQYVVSQIGGKGRVIACDILEMDPIVGVDFLQGDFRDENVLNALLARVGEDKVDV
CCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHHH
VMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLASKGSFVVKVFQGEGFDEYLREIRS
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHH
LFDVVKVRKPEASRGRSREVYIVATGYKG
HHHHHHHCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA