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The map label for this gene is greA

Identifier: 16273242

GI number: 16273242

Start: 1409061

End: 1409537

Strand: Direct

Name: greA

Synonym: HI1331

Alternate gene names: 16273242

Gene position: 1409061-1409537 (Clockwise)

Preceding gene: 16273238

Following gene: 16273244

Centisome position: 76.99

GC content: 36.69

Gene sequence:

>477_bases
ATGCAACAAATTCCAATGACTGTGCGTGGCGCAGAACAATTACGAGAAGAATTGGATTTCTTAAAAAATGTACGTCGTCC
AGAAATTATTAAAGCCATAGCAGAAGCCCGCGAGCACGGAGATTTAAAAGAAAATGCAGAATATCACGCAGCGCGTGAGC
AACAAGGTTTTTGTGAAGGTCGTATTCAAGAGATTGAAGGTAAACTTGGTAATGCTCAAATTATTGATGTCTCAAAAATG
CCAAATAATGGTAAAGTGATTTTTGGTGCAACAGTTGTGCTTGTAAATACAAATACAGACGAAGAAGTCACTTATCGCAT
TGTAGGCGATGATGAGGCTGATATTAAATCTGGTTTAATTTCGGTTAATTCACCAATTGCGCGAGGCTTAATTGGTAAAG
AATTAGATGATACCGTTAATATTACAACGCCTGGTGGTGTCGTTGAATTTGATATTATTGAAGTGAATTATATTTAA

Upstream 100 bases:

>100_bases
AGTAAATTATTTTCTCATTTCAAGTTTATTTTTGATAATACTGCAGTAAGATTTTAGAATTTTACTGTGGTTTTTGTTTT
ATTTAGTAAAAGGAAAAACA

Downstream 100 bases:

>100_bases
ATAAAAAAGTGCGGTTAAAAATGACCGCACTTTTGTTGTTATTTGCGTGGAAGCTGAATTTTTGCTTCTTCGCTTGGGCG
ATAAAGTACCAAAATATGCC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MQQIPMTVRGAEQLREELDFLKNVRRPEIIKAIAEAREHGDLKENAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVSKM
PNNGKVIFGATVVLVNTNTDEEVTYRIVGDDEADIKSGLISVNSPIARGLIGKELDDTVNITTPGGVVEFDIIEVNYI

Sequences:

>Translated_158_residues
MQQIPMTVRGAEQLREELDFLKNVRRPEIIKAIAEAREHGDLKENAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVSKM
PNNGKVIFGATVVLVNTNTDEEVTYRIVGDDEADIKSGLISVNSPIARGLIGKELDDTVNITTPGGVVEFDIIEVNYI
>Mature_158_residues
MQQIPMTVRGAEQLREELDFLKNVRRPEIIKAIAEAREHGDLKENAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVSKM
PNNGKVIFGATVVLVNTNTDEEVTYRIVGDDEADIKSGLISVNSPIARGLIGKELDDTVNITTPGGVVEFDIIEVNYI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=79.746835443038, Blast_Score=263, Evalue=5e-72,
Organism=Escherichia coli, GI87082261, Length=159, Percent_Identity=35.2201257861635, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_HAEIN (P43881)

Other databases:

- EMBL:   L42023
- PIR:   B64117
- RefSeq:   NP_439483.1
- ProteinModelPortal:   P43881
- SMR:   P43881
- GeneID:   950254
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1331
- NMPDR:   fig|71421.1.peg.1268
- TIGR:   HI_1331
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   HINF71421:HI_1331-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17478; Mature: 17478

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQIPMTVRGAEQLREELDFLKNVRRPEIIKAIAEAREHGDLKENAEYHAAREQQGFCEG
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
RIQEIEGKLGNAQIIDVSKMPNNGKVIFGATVVLVNTNTDEEVTYRIVGDDEADIKSGLI
HHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCHHHHHHCEE
SVNSPIARGLIGKELDDTVNITTPGGVVEFDIIEVNYI
ECCCHHHHHHCCCCCCCEEEEECCCCEEEEEEEEEECC
>Mature Secondary Structure
MQQIPMTVRGAEQLREELDFLKNVRRPEIIKAIAEAREHGDLKENAEYHAAREQQGFCEG
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
RIQEIEGKLGNAQIIDVSKMPNNGKVIFGATVVLVNTNTDEEVTYRIVGDDEADIKSGLI
HHHHHCCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCHHHHHHCEE
SVNSPIARGLIGKELDDTVNITTPGGVVEFDIIEVNYI
ECCCHHHHHHCCCCCCCEEEEECCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800