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The map label for this gene is truB

Identifier: 16273203

GI number: 16273203

Start: 1370058

End: 1370996

Strand: Direct

Name: truB

Synonym: HI1289

Alternate gene names: 16273203

Gene position: 1370058-1370996 (Clockwise)

Preceding gene: 16273202

Following gene: 16273204

Centisome position: 74.86

GC content: 42.64

Gene sequence:

>939_bases
ATGTCTAGACCTCGTAAACGCTGGCGTGATGTTGATGGTGTGTTTTTGTTGGATAAACCACAAGGCATGTCATCAAATGA
CATTATGCAAAAGGTAAAGCGTTTATTTCAAGCGAACAAAGCCGGGCATACTGGCGCGCTCGATCCGTTGGCTACGGGAA
TGTTGCCGATTTGCTTGGGGGAAGCAACTAAGTTTTCACAATTTTTGCTTGATGCCGATAAGCGTTATCTTGTTACGGCA
AAATTAGGCGAACGTACCGATACTTCCGATGCAGAAGGGCAAGTCGTGGAAACTCGTGAAGTTAATCTTGAAACTCAGCA
AATTTTGACCGCACTTGAACAATTTCGTGGCGATATTTTGCAAGTSCCGACAATGTTTTCCGCACTAAAACATAACGGCA
AACCGCTTTATGAATATGCGCGCCAAGGTATTACCGTTGAGCGTGAGGCACGTCCAATTACGATTTTTGAACTTAATTTT
ATTGAATATAATGCGCCTTTTTTAACCTTAGAAGTGCATTGTTCAAAAGGCACTTATATTCGCACCTTAGTAGATGATTT
AGGTGAAGTGCTAGGTTGTGGCGCGCATGTGACAATGTTACGCCGTACAGCCGTAGCGGATTACCCCGTAGCAGAAATGA
TGCCAATCAATGAGTTACAACTGCTCGCTGAAAGTTTTCCATTAAGTGAATTAGATCGTCTATTACTGCCAACCGATACC
GCAGTAAGTAAATTGCCCGCTTTGCATTTGGATGCAGAGCAGAGTAAAGCCATTGGTTTTGGTCAGCGAGTGAAATTTGC
TAACGAACAGCAATTAAGTGGTCAAGTGCGGTTATTTTCAGCGGAGAATTTATTCTTAGGCGTACTTAATCGACGGGAAT
ATTATTCGCCCACAACGATTAATTACACAATCCGCATAACTTCATTGCCTTTCCTGTAA

Upstream 100 bases:

>100_bases
TTATTTACGATCAATCCTTAGTGGAAGGGATGCGTATGTCAAATCTTGTGACAAATGTTGTGCGTGAAGATGAGAAAAAA
CACGTTGAGGAAAGCAACTA

Downstream 100 bases:

>100_bases
AATTCTAGTAATCTCCTTGTAATTAATTTTTTACAATATATGTAAGATAAAAATTACAGGTGAGTTTTATGGAAGCCCTT
AAAGATTTACGTTCTGAAAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYLVTA
KLGERTDTSDAEGQVVETREVNLETQQILTALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNF
IEYNAPFLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDT
AVSKLPALHLDAEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVLNRREYYSPTTINYTIRITSLPFL

Sequences:

>Translated_312_residues
MSRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYLVTA
KLGERTDTSDAEGQVVETREVNLETQQILTALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNF
IEYNAPFLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDT
AVSKLPALHLDAEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVLNRREYYSPTTINYTIRITSLPFL
>Mature_311_residues
SRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYLVTAK
LGERTDTSDAEGQVVETREVNLETQQILTALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNFI
EYNAPFLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDTA
VSKLPALHLDAEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVLNRREYYSPTTINYTIRITSLPFL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=35.9090909090909, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI4503337, Length=173, Percent_Identity=30.635838150289, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI215599015, Length=173, Percent_Identity=30.635838150289, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI2367200, Length=295, Percent_Identity=60, Blast_Score=352, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17553978, Length=193, Percent_Identity=30.5699481865285, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=193, Percent_Identity=31.0880829015544, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=189, Percent_Identity=31.7460317460317, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281364189, Length=192, Percent_Identity=29.6875, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281364187, Length=192, Percent_Identity=29.6875, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281364185, Length=192, Percent_Identity=29.6875, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281364183, Length=192, Percent_Identity=29.6875, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI62471759, Length=192, Percent_Identity=29.6875, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17975520, Length=192, Percent_Identity=29.6875, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_HAEIN (P45142)

Other databases:

- EMBL:   L42023
- PIR:   B64170
- RefSeq:   NP_439441.1
- ProteinModelPortal:   P45142
- SMR:   P45142
- GeneID:   950222
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1289
- NMPDR:   fig|71421.1.peg.1229
- TIGR:   HI_1289
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK05033
- BioCyc:   HINF71421:HI_1289-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 35086; Mature: 34955

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLG
CCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDADKRYLVTAKLGERTDTSDAEGQVVETREVNLETQQILTALEQFRGDIL
CHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHH
QVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNFIEYNAPFLTLEVHCSKGTYI
HHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCEEEEEEEECCCCCH
RTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDT
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCH
AVSKLPALHLDAEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVLNRREYYSPTTI
HHHHCCEEEECCCHHHHCCCCCCCCCCCCHHCCCEEEEEECCCEEEEECCCCCCCCCCEE
NYTIRITSLPFL
EEEEEEEECCCC
>Mature Secondary Structure 
SRPRKRWRDVDGVFLLDKPQGMSSNDIMQKVKRLFQANKAGHTGALDPLATGMLPICLG
CCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDADKRYLVTAKLGERTDTSDAEGQVVETREVNLETQQILTALEQFRGDIL
CHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHH
QVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELNFIEYNAPFLTLEVHCSKGTYI
HHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCEEEEEEEECCCCCH
RTLVDDLGEVLGCGAHVTMLRRTAVADYPVAEMMPINELQLLAESFPLSELDRLLLPTDT
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCH
AVSKLPALHLDAEQSKAIGFGQRVKFANEQQLSGQVRLFSAENLFLGVLNRREYYSPTTI
HHHHCCEEEECCCHHHHCCCCCCCCCCCCHHCCCEEEEEECCCEEEEECCCCCCCCCCEE
NYTIRITSLPFL
EEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800